FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158444308> ?p ?o ?g. }

• W2158444308 endingPage "129" @default.
• W2158444308 startingPage "118" @default.
• W2158444308 abstract "12-lipoxygenase-catalyzed arachidonic acid metabolism in normal and neoplastic mouse epidermis was assessed by cDNA cloning of the epidermal 12-lipoxygenases and by studying their expression patterns, enzyme activities, and product levels. Papillomas and squamous cell carcinomas induced by the initiation/promotion protocol contained 50-to 60-fold more 12-hydroxy-5,8,10,14-eicosatetraenoic acid (a) than normal epidermis. The ratio of S to R enantiomers was 9:1. This indicates that most of this eicosanoid was of enzymatic origin. Accordingly, cell-free preparations of the tumors exhibited about fivefold elevated 12-lipoxygenase activities. A papilloma-derived cDNA library was screened with human platelet-type 12-lipoxygenase cDNA probes. Two cDNA clones encoding the platelet-type and the leukocyte-type isoforms of murine 12-lipoxygenase were isolated, demonstrating the coexpression of the isoenzymes in the same tissue and species. When expressed in COS-7 cells, the recombinant enzymes showed the characteristic substrate selectivity and product profile, with the leukocyte-type enzyme metabolizing linoleic and arachidonic acid to 13-hydroxy-9,11-octadecadienoic acid and to 12- and 15-HETE, respectively, and the platelet-type enzyme oxygenating exclusively arachidonic acid to 12-HETE. In epidermis in vivo and in keratinocytes in culture, only the platelet-type 12-lipoxygenase (mRNA and protein) was detectable. In mouse epidermis both isoenzymes were induced transiently by phorbol esters. Most tumors showed constitutive overexpression of platelet-type mRNA, whereas leukocyte-type specific transcripts were detectable only in a few tumors. These data suggest that the platelet-type enzyme is the 12-lipoxygenase isoform of keratinocytes that is responsible for the generation of most of the 12-HETE found in neoplastic epidermis. ©1995 Wiley-Liss, Inc." @default.
• W2158444308 created "2016-06-24" @default.
• W2158444308 creator A5004518388 @default.
• W2158444308 creator A5007487924 @default.
• W2158444308 creator A5010225884 @default.
• W2158444308 creator A5032579502 @default.
• W2158444308 creator A5037161101 @default.
• W2158444308 creator A5043694861 @default.
• W2158444308 creator A5051433372 @default.
• W2158444308 creator A5052837527 @default.
• W2158444308 creator A5080601032 @default.
• W2158444308 date "1995-10-01" @default.
• W2158444308 modified "2023-09-26" @default.
• W2158444308 title "12-lipoxygenase isoenzymes in mouse skin tumor development" @default.
• W2158444308 cites W107907750 @default.
• W2158444308 cites W1504633391 @default.
• W2158444308 cites W1510480381 @default.
• W2158444308 cites W1540646729 @default.
• W2158444308 cites W1560539518 @default.
• W2158444308 cites W1567180128 @default.
• W2158444308 cites W1968792529 @default.
• W2158444308 cites W1969104374 @default.
• W2158444308 cites W1969153299 @default.
• W2158444308 cites W1984373476 @default.
• W2158444308 cites W1987440586 @default.
• W2158444308 cites W1990735928 @default.
• W2158444308 cites W1990807364 @default.
• W2158444308 cites W1998724709 @default.
• W2158444308 cites W2011374830 @default.
• W2158444308 cites W2012254133 @default.
• W2158444308 cites W2030202421 @default.
• W2158444308 cites W2031313177 @default.
• W2158444308 cites W2033052130 @default.
• W2158444308 cites W2037415808 @default.
• W2158444308 cites W2040276985 @default.
• W2158444308 cites W2041592072 @default.
• W2158444308 cites W2041768024 @default.
• W2158444308 cites W2056501353 @default.
• W2158444308 cites W2066873884 @default.
• W2158444308 cites W2073088363 @default.
• W2158444308 cites W2074231493 @default.
• W2158444308 cites W2077134680 @default.
• W2158444308 cites W2080216367 @default.
• W2158444308 cites W2083517465 @default.
• W2158444308 cites W2084997952 @default.
• W2158444308 cites W2094031081 @default.
• W2158444308 cites W2096952719 @default.
• W2158444308 cites W2100837269 @default.
• W2158444308 cites W2101108802 @default.
• W2158444308 cites W2155736383 @default.
• W2158444308 cites W2412341010 @default.
• W2158444308 cites W3146714045 @default.
• W2158444308 cites W327331692 @default.
• W2158444308 doi "https://doi.org/10.1002/mc.2940140208" @default.
• W2158444308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7576099" @default.
• W2158444308 hasPublicationYear "1995" @default.
• W2158444308 type Work @default.
• W2158444308 sameAs 2158444308 @default.
• W2158444308 citedByCount "80" @default.
• W2158444308 countsByYear W21584443082012 @default.
• W2158444308 countsByYear W21584443082013 @default.
• W2158444308 countsByYear W21584443082014 @default.
• W2158444308 countsByYear W21584443082015 @default.
• W2158444308 countsByYear W21584443082016 @default.
• W2158444308 countsByYear W21584443082018 @default.
• W2158444308 countsByYear W21584443082021 @default.
• W2158444308 countsByYear W21584443082022 @default.
• W2158444308 crossrefType "journal-article" @default.
• W2158444308 hasAuthorship W2158444308A5004518388 @default.
• W2158444308 hasAuthorship W2158444308A5007487924 @default.
• W2158444308 hasAuthorship W2158444308A5010225884 @default.
• W2158444308 hasAuthorship W2158444308A5032579502 @default.
• W2158444308 hasAuthorship W2158444308A5037161101 @default.
• W2158444308 hasAuthorship W2158444308A5043694861 @default.
• W2158444308 hasAuthorship W2158444308A5051433372 @default.
• W2158444308 hasAuthorship W2158444308A5052837527 @default.
• W2158444308 hasAuthorship W2158444308A5080601032 @default.
• W2158444308 hasConcept C104317684 @default.
• W2158444308 hasConcept C105702510 @default.
• W2158444308 hasConcept C119795356 @default.
• W2158444308 hasConcept C153911025 @default.
• W2158444308 hasConcept C181199279 @default.
• W2158444308 hasConcept C187882448 @default.
• W2158444308 hasConcept C2776458125 @default.
• W2158444308 hasConcept C2778078955 @default.
• W2158444308 hasConcept C2780857362 @default.
• W2158444308 hasConcept C55493867 @default.
• W2158444308 hasConcept C86803240 @default.
• W2158444308 hasConceptScore W2158444308C104317684 @default.
• W2158444308 hasConceptScore W2158444308C105702510 @default.
• W2158444308 hasConceptScore W2158444308C119795356 @default.
• W2158444308 hasConceptScore W2158444308C153911025 @default.
• W2158444308 hasConceptScore W2158444308C181199279 @default.
• W2158444308 hasConceptScore W2158444308C187882448 @default.
• W2158444308 hasConceptScore W2158444308C2776458125 @default.
• W2158444308 hasConceptScore W2158444308C2778078955 @default.
• W2158444308 hasConceptScore W2158444308C2780857362 @default.
• W2158444308 hasConceptScore W2158444308C55493867 @default.

• next