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• W2158519702 abstract "Abstract Background It was recently established that changes in methylation during development are dynamic and involve both methylation and demethylation processes. Yet, which genomic sites are changing and what are the contributions of methylation (5mC) and hydroxymethylation (5hmC) to this epigenetic remodeling is still unknown. When studying early development, options for methylation profiling are limited by the unavailability of sufficient DNA material from these scarce samples and limitations are aggravated in non-model species due to the lack of technological platforms. We therefore sought to obtain a representation of differentially 5mC or 5hmC loci during bovine early embryo stages through the use of three complementary methods, based on selective methyl-sensitive restriction and enrichment by ligation-mediated PCR or on subtractive hybridization. Using these strategies, libraries of putative methylation and hydroxymethylated sites were generated from Day-7 and Day-12 bovine embryos. Results Over 1.2 million sequencing reads were analyzed, resulting in 151,501 contigs, of which 69,136 were uniquely positioned on the genome. A total of 101,461 putative methylated sites were identified. The output of the three methods differed in genomic coverage as well as in the nature of the identified sites. The classical MspI/HpaII combination of restriction enzymes targeted CpG islands whereas the other methods covered 5mC and 5hmC sites outside of these regions. Data analysis suggests a transition of these methylation marks between Day-7 and Day-12 embryos in specific classes of repeat-containing elements. Conclusions Our combined strategy offers a genomic map of the distribution of cytosine methylation/hydroxymethylation during early bovine embryo development. These results support the hypothesis of a regulatory phase of hypomethylation in repeat sequences during early embryogenesis." @default.
• W2158519702 created "2016-06-24" @default.
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• W2158519702 date "2013-06-18" @default.
• W2158519702 modified "2023-10-06" @default.
• W2158519702 title "Combined methylation mapping of 5mC and 5hmC during early embryonic stages in bovine" @default.
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• W2158519702 cites W1502815369 @default.
• W2158519702 cites W1521888731 @default.
• W2158519702 cites W1575368626 @default.
• W2158519702 cites W1968271489 @default.
• W2158519702 cites W1969634007 @default.
• W2158519702 cites W1970471513 @default.
• W2158519702 cites W1971157495 @default.
• W2158519702 cites W1973727308 @default.
• W2158519702 cites W1979606094 @default.
• W2158519702 cites W1981463058 @default.
• W2158519702 cites W1981543052 @default.
• W2158519702 cites W1989457196 @default.
• W2158519702 cites W1990543456 @default.
• W2158519702 cites W1992834799 @default.
• W2158519702 cites W1993981510 @default.
• W2158519702 cites W1997796525 @default.
• W2158519702 cites W1997849784 @default.
• W2158519702 cites W2005453259 @default.
• W2158519702 cites W2005458309 @default.
• W2158519702 cites W2010468147 @default.
• W2158519702 cites W2011244430 @default.
• W2158519702 cites W2011556073 @default.
• W2158519702 cites W2014039097 @default.
• W2158519702 cites W2020016210 @default.
• W2158519702 cites W2020887634 @default.
• W2158519702 cites W2024312710 @default.
• W2158519702 cites W2029654103 @default.
• W2158519702 cites W2030687162 @default.
• W2158519702 cites W2037920286 @default.
• W2158519702 cites W2038449573 @default.
• W2158519702 cites W2038722690 @default.
• W2158519702 cites W2038756976 @default.
• W2158519702 cites W2044282102 @default.
• W2158519702 cites W2047927260 @default.
• W2158519702 cites W2050345155 @default.
• W2158519702 cites W2051758546 @default.
• W2158519702 cites W2053856666 @default.
• W2158519702 cites W2056738426 @default.
• W2158519702 cites W2059692367 @default.
• W2158519702 cites W2060822442 @default.
• W2158519702 cites W2061086857 @default.
• W2158519702 cites W2066523495 @default.
• W2158519702 cites W2069717852 @default.
• W2158519702 cites W2071501400 @default.
• W2158519702 cites W2078057013 @default.
• W2158519702 cites W2081870901 @default.
• W2158519702 cites W2089253191 @default.
• W2158519702 cites W2096391240 @default.
• W2158519702 cites W2101780023 @default.
• W2158519702 cites W2102619694 @default.
• W2158519702 cites W2103486606 @default.
• W2158519702 cites W2104146295 @default.
• W2158519702 cites W2104429441 @default.
• W2158519702 cites W2108664872 @default.
• W2158519702 cites W2109715726 @default.
• W2158519702 cites W2112448007 @default.
• W2158519702 cites W2115936826 @default.
• W2158519702 cites W2117464640 @default.
• W2158519702 cites W2124351063 @default.
• W2158519702 cites W2125916700 @default.
• W2158519702 cites W2133620956 @default.
• W2158519702 cites W2135556539 @default.
• W2158519702 cites W2135814599 @default.
• W2158519702 cites W2136145671 @default.
• W2158519702 cites W2137983089 @default.
• W2158519702 cites W2142904226 @default.
• W2158519702 cites W2143798330 @default.
• W2158519702 cites W2144419479 @default.
• W2158519702 cites W2150393318 @default.
• W2158519702 cites W2160704282 @default.
• W2158519702 cites W2165178639 @default.
• W2158519702 cites W2167827709 @default.
• W2158519702 cites W2170147098 @default.
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• W2158519702 doi "https://doi.org/10.1186/1471-2164-14-406" @default.
• W2158519702 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3689598" @default.
• W2158519702 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23773395" @default.
• W2158519702 hasPublicationYear "2013" @default.
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