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- W2158530838 abstract "A 19-kDa protein, Pin m III, was recently shown to be associated with overwintering and frost hardiness of western white pine (Pinus monticola Dougl. ex D. Don) seedlings. Here, we report that this protein is up-regulated by the fungus Cronartium ribicola Fisch, the causal agent of white pine blister rust in western white pine trees. Between 1991 and 1994, bark samples of mature western white pine trees (resistant with no stem cankers and susceptible with stem cankers) were collected in winter, spring, and fall. Proteins were extracted and analyzed by Western immunoblot utilizing specific rabbit polyclonal anti-Pin l I (a homologue of Pin m III) antibodies. During all collection dates, but particularly in the spring, susceptible trees had more Pin m III than resistant trees. In July 1995, 43 previously inoculated 7-year-old white pine seedlings were also analyzed. In all susceptible seedlings (cankered) tested, cankered tissue had high levels of Pin m III, and samples collected from the outside edge of th..." @default.
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- W2158530838 date "1998-01-01" @default.
- W2158530838 modified "2023-09-27" @default.
- W2158530838 title "A protein associated with frost hardiness of western white pine is up-regulated by infection in the white pine blister rust pathosystem" @default.
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- W2158530838 doi "https://doi.org/10.1139/cjfr-28-3-412" @default.
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