FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158541229> ?p ?o ?g. }

• W2158541229 abstract "Mucins are large and highly glycosylated proteins and major component of the mucus that coats the lining of epithelial organs. Mucins are characterized by the presence of extended regions rich in the amino acids Proline, Threonine and Serine (PTS domain), where the Serines and Threonines are O-glycosylated to form sugar-rich mucin domains. Mucins are classified into secreted gel-forming mucins and transmembrane mucins with possible signaling functions. The amino acid sequence of the PTS domains tends to be poorly conserved between species and different mucins. The goal of this thesis was to identify and study potential mucin-like proteins in Drosophila melanogaster. We devised a simple bioinformatic approach and developed a program that can identify PTS domains based on amino acid content. We thereby identified 36 mucins and mucin-related proteins. All proteins appear to be secreted, except for two that harbor a predicted transmembrane domain. Expression analysis at different stages of the Drosophila life cycle revealed that many mucins are expressed in the larval gut, consistent with a function in mucosal barrier formation. Interestingly, some of the mucins showed dynamic expression in different tubular organs during embryogenesis. Among these was Mur96B/Tenectin (Tnc) that was further studied to dissect its role in epithelial organ development. We found that Tnc is critical for diameter expansion of the developing hindgut. Tnc forms a transient matrix that fills the hindgut lumen and drives expansion in a dose-dependent manner, presumably by generating a luminal pressure. This study revealed a new mechanism in organ development, whereby the extent of lumen volume expansion can be regulated by the accumulation of single glycoprotein. In parallel to the bioinformatic approach, we identified a Drosophila protein that shares conserved domains with human SUSD2 and the non-mucin parts of human MUC4, called Mesh. We aimed to analyze Mesh function as a means to address the roles of these domains. Mesh was found to be is expressed in the digestive tract epithelium from mid-embryogenesis and throughout larval and adult life, localizing to the apical junction belt. Mesh is required for correct organization of the Scribble-complex, a main polarity complex conserved between fly and mammals, to prevent excess expansion of apical cell surface and for microvilli organization. The results demonstrate that mucin-like proteins, containing the PTS domains or other mucin-related domains, are essential for epithelial organ development in Drosophila." @default.
• W2158541229 created "2016-06-24" @default.
• W2158541229 creator A5058752490 @default.
• W2158541229 date "2014-01-17" @default.
• W2158541229 modified "2023-09-27" @default.
• W2158541229 title "Mucin-like proteins in Drosophila development" @default.
• W2158541229 cites W107411923 @default.
• W2158541229 cites W107544894 @default.
• W2158541229 cites W1225109155 @default.
• W2158541229 cites W1484551801 @default.
• W2158541229 cites W1521774859 @default.
• W2158541229 cites W1535427487 @default.
• W2158541229 cites W1548284679 @default.
• W2158541229 cites W1554042779 @default.
• W2158541229 cites W1560056935 @default.
• W2158541229 cites W1560175476 @default.
• W2158541229 cites W15851850 @default.
• W2158541229 cites W1589735909 @default.
• W2158541229 cites W1595817144 @default.
• W2158541229 cites W1647075334 @default.
• W2158541229 cites W1820870565 @default.
• W2158541229 cites W188704096 @default.
• W2158541229 cites W1931392437 @default.
• W2158541229 cites W1966032423 @default.
• W2158541229 cites W1966080460 @default.
• W2158541229 cites W1970391453 @default.
• W2158541229 cites W1970698118 @default.
• W2158541229 cites W1972117643 @default.
• W2158541229 cites W1972577951 @default.
• W2158541229 cites W1974405231 @default.
• W2158541229 cites W1975097039 @default.
• W2158541229 cites W1975338046 @default.
• W2158541229 cites W1976226144 @default.
• W2158541229 cites W1976609927 @default.
• W2158541229 cites W1978081193 @default.
• W2158541229 cites W1979312850 @default.
• W2158541229 cites W1980979311 @default.
• W2158541229 cites W1982645824 @default.
• W2158541229 cites W1985016907 @default.
• W2158541229 cites W1985296504 @default.
• W2158541229 cites W1986905652 @default.
• W2158541229 cites W1988518541 @default.
• W2158541229 cites W1990105468 @default.
• W2158541229 cites W1990188178 @default.
• W2158541229 cites W1992615556 @default.
• W2158541229 cites W1993991804 @default.
• W2158541229 cites W1995108114 @default.
• W2158541229 cites W1995420663 @default.
• W2158541229 cites W1996070154 @default.
• W2158541229 cites W2003885329 @default.
• W2158541229 cites W2004296273 @default.
• W2158541229 cites W2007191876 @default.
• W2158541229 cites W2008555242 @default.
• W2158541229 cites W2008588429 @default.
• W2158541229 cites W2010605796 @default.
• W2158541229 cites W2012569825 @default.
• W2158541229 cites W2015217831 @default.
• W2158541229 cites W2015549113 @default.
• W2158541229 cites W2015853073 @default.
• W2158541229 cites W2016270258 @default.
• W2158541229 cites W2018440302 @default.
• W2158541229 cites W2018574900 @default.
• W2158541229 cites W2019614403 @default.
• W2158541229 cites W2019708066 @default.
• W2158541229 cites W2021580403 @default.
• W2158541229 cites W2021649658 @default.
• W2158541229 cites W2024924891 @default.
• W2158541229 cites W2026842789 @default.
• W2158541229 cites W2027119249 @default.
• W2158541229 cites W2027125425 @default.
• W2158541229 cites W2027856108 @default.
• W2158541229 cites W2029942527 @default.
• W2158541229 cites W2030317873 @default.
• W2158541229 cites W2031223021 @default.
• W2158541229 cites W2031720224 @default.
• W2158541229 cites W2033121774 @default.
• W2158541229 cites W2033123952 @default.
• W2158541229 cites W2034108485 @default.
• W2158541229 cites W2034696312 @default.
• W2158541229 cites W2035818114 @default.
• W2158541229 cites W2036051953 @default.
• W2158541229 cites W2037128019 @default.
• W2158541229 cites W2039295111 @default.
• W2158541229 cites W2040988046 @default.
• W2158541229 cites W2041677910 @default.
• W2158541229 cites W2041711980 @default.
• W2158541229 cites W2041787605 @default.
• W2158541229 cites W2042488865 @default.
• W2158541229 cites W2045036647 @default.
• W2158541229 cites W2046756714 @default.
• W2158541229 cites W2046850350 @default.
• W2158541229 cites W2050557699 @default.
• W2158541229 cites W2051508448 @default.
• W2158541229 cites W2051604067 @default.
• W2158541229 cites W2052254968 @default.
• W2158541229 cites W2054268736 @default.
• W2158541229 cites W2055047934 @default.
• W2158541229 cites W2055083701 @default.
• W2158541229 cites W2056494900 @default.
• W2158541229 cites W2058815724 @default.

• next