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- W2158608942 abstract "In vitro incubation of bovine Cu,Zn-SOD (Cu,Zn-superoxide dismutase) with glucose, ribose or fructose results in a remarkable inactivation of the enzyme. There was a progressive decrease in enzyme activity on incubation with glucose and, at the end of 7 days, only 26% of the initial activity remained. The inactivation was accompanied by a parallel decrease in the amount of protein detectable on gels after SDS/PAGE. Reaction of the sugars with SOD was ascertained using an immunoblot assay in which sugar-incubated SOD was derivatized with 2,4-dinitrophenylhydrazine and allowed to react with the dinitrophenol-specific antibody. Affinity purified antibodies from the sera of rabbits immunized with bovine SOD were highly effective in restricting the inactivation of the enzyme induced by glucose, ribose or fructose." @default.
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- W2158608942 date "2006-01-01" @default.
- W2158608942 modified "2023-09-23" @default.
- W2158608942 title "Polyclonal antibodies inhibit the glycation-induced inactivation of bovine Cu,Zn-superoxide dismutase" @default.
- W2158608942 doi "https://doi.org/10.1042/ba20040201" @default.
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