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- W2158693995 abstract "(i) To determine the presence of low and high molecular weight cell-free DNA in seminal plasma and: (ii) To correlate the amounts of cell-free DNA with sperm parameters. The capillary electrophoresis (CE) method was modified to provide a rapid assay for cell-free DNA in semen obtained from 25 male patients attending the fertility clinic. The results were correlated to important sperm function parameters. Advanced semen analyses were performed on 25 specimens. Fresh and frozen-thawed seminal plasma were each separately pipetted inside glass micro- hematocrit capillary tubes (internal diameter 1.2 mm, wall 0.2mm) half-filled with a 2.4 % agarose gel and sealed with Liquasonic conducting gel. Control capillary tubes with either buffer, female sera or HaeIII-phiX DNA markers were also included. Horizontal electrophoresis (100 volts, 24 mins, TAE buffer)was carried out and the agarose column from each capillary tube was stained in Sybr-Gold (5 mins), rinsed, and analyzed under UV-epifluorescence. Digital images from gel sections corresponding to high (12 Kb) and low (1 Kb) molecular weight DNA fragments were analyzed. Results were expressed as a ratio of the DNA intensity to the control and significance tested using linear regression and Student's t-test. The amount of low molecular weight cell-free DNA was directly correlated to rapid progression, curvilinear velocity (> 40 μ/sec), normal strict morphology and the sperm penetration assay capacitation index. In contrast, cell-free DNA quantity was negatively correlated to post-wash hyperactivation. Sperm concentration was not related to cell-free DNA quantity. Freezing semen did not increase cell-free DNA but decreased the low molecular weight cell-free DNA. Cell-free DNA present in semen was related to important sperm parameters linked to normal sperm function. The data suggested the possible use of low molecular weight cell-free DNA as a marker of semen quality. This study reports on the novel finding of cell-free DNA released along with sperm during each ejaculation.View Large Image Figure ViewerDownload Hi-res image Download (PPT)" @default.
- W2158693995 created "2016-06-24" @default.
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- W2158693995 date "2004-09-01" @default.
- W2158693995 modified "2023-09-26" @default.
- W2158693995 title "Capillary electrophoresis of seminal plasma links cell-free DNA in semen to sperm quality" @default.
- W2158693995 doi "https://doi.org/10.1016/j.fertnstert.2004.07.477" @default.
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