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• W2158805406 abstract "The effectiveness of two new supercharging reagents for producing highly charged ions by electrospray ionization (ESI) from aqueous solutions in which proteins have native structures and reactivities were investigated. In aqueous solution, 2-thiophenone and 4-hydroxymethyl-1,3-dioxolan-2-one (HD) at a concentration of 2% by volume can increase the average charge of cytochrome c and myoglobin by up to 163%, resulting in even higher charge states than those that are produced from water/methanol/acid solutions in which these proteins are denatured. The greatest extent of supercharging occurs in pure water, but these supercharging reagents are also highly effective in aqueous solutions containing 200 mM ammonium acetate buffer commonly used in native mass spectrometry (MS). These reagents are less effective supercharging reagents than m-nitrobenzyl alcohol (m-NBA) and propylene carbonate (PC) when ions are formed from water/methanol/acid. The extent to which loss of the heme group from myoglobin occurs is related to the extent of supercharging. Results from guanidine melts of cytochrome c monitored with tryptophan fluorescence show that the supercharging reagents PC, sulfolane and HD are effective chemical denaturants in solution. These results provide additional evidence for the role of protein structural changes in the electrospray droplet as the primary mechanism for supercharging with these reagents in native MS. These results also demonstrate that for at least some proteins, the formation of highly charged ions from native MS is no longer a significant barrier for obtaining structural information using conventional tandem MS methods." @default.
• W2158805406 created "2016-06-24" @default.
• W2158805406 creator A5014296444 @default.
• W2158805406 creator A5059657134 @default.
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• W2158805406 date "2015-01-01" @default.
• W2158805406 modified "2023-10-01" @default.
• W2158805406 title "New supercharging reagents produce highly charged protein ions in native mass spectrometry" @default.
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• W2158805406 cites W1553635068 @default.
• W2158805406 cites W1903368602 @default.
• W2158805406 cites W1965559022 @default.
• W2158805406 cites W1973163422 @default.
• W2158805406 cites W1973333093 @default.
• W2158805406 cites W1975599463 @default.
• W2158805406 cites W1979627609 @default.
• W2158805406 cites W1980310871 @default.
• W2158805406 cites W1984457845 @default.
• W2158805406 cites W1986709132 @default.
• W2158805406 cites W1991404185 @default.
• W2158805406 cites W1991998914 @default.
• W2158805406 cites W1992276902 @default.
• W2158805406 cites W1995113829 @default.
• W2158805406 cites W1997645853 @default.
• W2158805406 cites W1997980075 @default.
• W2158805406 cites W1998225250 @default.
• W2158805406 cites W2000640600 @default.
• W2158805406 cites W2003436145 @default.
• W2158805406 cites W2005046688 @default.
• W2158805406 cites W2006244553 @default.
• W2158805406 cites W2008876483 @default.
• W2158805406 cites W2010118308 @default.
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• W2158805406 cites W2024849484 @default.
• W2158805406 cites W2027440965 @default.
• W2158805406 cites W2029794817 @default.
• W2158805406 cites W2032579944 @default.
• W2158805406 cites W2034101306 @default.
• W2158805406 cites W2035192734 @default.
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• W2158805406 cites W2044702083 @default.
• W2158805406 cites W2045268196 @default.
• W2158805406 cites W2053407332 @default.
• W2158805406 cites W2058794436 @default.
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• W2158805406 cites W2063896210 @default.
• W2158805406 cites W2065843920 @default.
• W2158805406 cites W2068248648 @default.
• W2158805406 cites W2073389004 @default.
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• W2158805406 doi "https://doi.org/10.1039/c5an01710f" @default.
• W2158805406 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4617834" @default.
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