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• W2158830887 abstract "ABSTRACT In this study we describe a multiplex PCR assay for the detection of nine clinically relevant antibiotic resistance genes of Staphylococcus aureus. Conditions were optimized to amplify fragments of mecA (encoding methicillin resistance), aacA-aphD (aminoglycoside resistance), tetK , tetM (tetracycline resistance), erm (A), erm (C) (macrolide-lincosamide-streptogramin B resistance), vat (A), vat (B), and vat (C) (streptogramin A resistance) simultaneously in one PCR amplification. An additional primer pair for the amplification of a fragment of the staphylococcal 16S rDNA was included as a positive control. The multiplex PCR assay was evaluated on 30 different S. aureus isolates, and the PCR results correlated with the phenotypic antibiotic resistance data obtained by the broth microdilution assay. The multiplex PCR assay offers a rapid, simple, and accurate identification of antibiotic resistance profiles and could be used in clinical diagnosis as well as for the surveillance of the spread of antibiotic resistance determinants in epidemiological studies." @default.
• W2158830887 created "2016-06-24" @default.
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• W2158830887 date "2003-09-01" @default.
• W2158830887 modified "2023-10-01" @default.
• W2158830887 title "Multiplex PCR Assay for Simultaneous Detection of Nine Clinically Relevant Antibiotic Resistance Genes in <i>Staphylococcus aureus</i>" @default.
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• W2158830887 doi "https://doi.org/10.1128/jcm.41.9.4089-4094.2003" @default.
• W2158830887 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/193808" @default.
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