Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158959097> ?p ?o ?g. }
- W2158959097 endingPage "257" @default.
- W2158959097 startingPage "247" @default.
- W2158959097 abstract "BACE457 is a recently identified pancreatic isoform of human β-secretase. We report that this membrane glycoprotein and its soluble variant are characterized by inefficient folding in the ER, leading to proteasome-mediated ER-associated degradation (ERAD). Dissection of the degradation process revealed that upon release from calnexin, extensively oxidized BACE457 transiently entered in disulfide-bonded complexes associated with the lumenal chaperones BiP and protein disulfide isomerase (PDI) before unfolding and dislocation into the cytosol for degradation. BACE457 and its lumenal variant accumulated in disulfide-bonded complexes, in the ER lumen, also when protein degradation was inhibited. The complexes were disassembled and the misfolded polypeptides were cleared from the ER upon reactivation of the degradation machinery. Our data offer new insights into the mechanism of ERAD by showing a sequential involvement of the calnexin and BiP/PDI chaperone systems. We report the unexpected transient formation of covalent complexes in the ER lumen during the ERAD process, and we show that PDI participates as an oxidoreductase and a redox-driven chaperone in the preparation of proteins for degradation from the mammalian ER." @default.
- W2158959097 created "2016-06-24" @default.
- W2158959097 creator A5012945125 @default.
- W2158959097 creator A5066985286 @default.
- W2158959097 creator A5073899091 @default.
- W2158959097 creator A5082150426 @default.
- W2158959097 creator A5082876418 @default.
- W2158959097 date "2002-07-15" @default.
- W2158959097 modified "2023-09-28" @default.
- W2158959097 title "Sequential assistance of molecular chaperones and transient formation of covalent complexes during protein degradation from the ER" @default.
- W2158959097 cites W1485214242 @default.
- W2158959097 cites W1563724009 @default.
- W2158959097 cites W1578569374 @default.
- W2158959097 cites W1582229352 @default.
- W2158959097 cites W1592548488 @default.
- W2158959097 cites W1607176255 @default.
- W2158959097 cites W1644122431 @default.
- W2158959097 cites W1915442041 @default.
- W2158959097 cites W1930219409 @default.
- W2158959097 cites W1965901600 @default.
- W2158959097 cites W1966415110 @default.
- W2158959097 cites W1976601347 @default.
- W2158959097 cites W1980775568 @default.
- W2158959097 cites W1981284304 @default.
- W2158959097 cites W1986129246 @default.
- W2158959097 cites W1987482757 @default.
- W2158959097 cites W1991396089 @default.
- W2158959097 cites W1997369314 @default.
- W2158959097 cites W1997801394 @default.
- W2158959097 cites W2000319181 @default.
- W2158959097 cites W2000806214 @default.
- W2158959097 cites W2005145242 @default.
- W2158959097 cites W2008822238 @default.
- W2158959097 cites W2009664225 @default.
- W2158959097 cites W2028603831 @default.
- W2158959097 cites W2039863802 @default.
- W2158959097 cites W2040666005 @default.
- W2158959097 cites W2045893780 @default.
- W2158959097 cites W2048737737 @default.
- W2158959097 cites W2048984147 @default.
- W2158959097 cites W2053026504 @default.
- W2158959097 cites W2062482281 @default.
- W2158959097 cites W2086142693 @default.
- W2158959097 cites W2105393355 @default.
- W2158959097 cites W2112326894 @default.
- W2158959097 cites W2114953435 @default.
- W2158959097 cites W2117313136 @default.
- W2158959097 cites W2121217914 @default.
- W2158959097 cites W2133019124 @default.
- W2158959097 cites W2139786644 @default.
- W2158959097 cites W2162815565 @default.
- W2158959097 cites W2169152986 @default.
- W2158959097 cites W2170117828 @default.
- W2158959097 cites W4234635809 @default.
- W2158959097 doi "https://doi.org/10.1083/jcb.200204122" @default.
- W2158959097 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2173128" @default.
- W2158959097 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12119363" @default.
- W2158959097 hasPublicationYear "2002" @default.
- W2158959097 type Work @default.
- W2158959097 sameAs 2158959097 @default.
- W2158959097 citedByCount "211" @default.
- W2158959097 countsByYear W21589590972012 @default.
- W2158959097 countsByYear W21589590972013 @default.
- W2158959097 countsByYear W21589590972014 @default.
- W2158959097 countsByYear W21589590972015 @default.
- W2158959097 countsByYear W21589590972016 @default.
- W2158959097 countsByYear W21589590972017 @default.
- W2158959097 countsByYear W21589590972018 @default.
- W2158959097 countsByYear W21589590972019 @default.
- W2158959097 countsByYear W21589590972020 @default.
- W2158959097 countsByYear W21589590972021 @default.
- W2158959097 countsByYear W21589590972022 @default.
- W2158959097 countsByYear W21589590972023 @default.
- W2158959097 crossrefType "journal-article" @default.
- W2158959097 hasAuthorship W2158959097A5012945125 @default.
- W2158959097 hasAuthorship W2158959097A5066985286 @default.
- W2158959097 hasAuthorship W2158959097A5073899091 @default.
- W2158959097 hasAuthorship W2158959097A5082150426 @default.
- W2158959097 hasAuthorship W2158959097A5082876418 @default.
- W2158959097 hasBestOaLocation W21589590971 @default.
- W2158959097 hasConcept C12554922 @default.
- W2158959097 hasConcept C130361206 @default.
- W2158959097 hasConcept C139447449 @default.
- W2158959097 hasConcept C142724271 @default.
- W2158959097 hasConcept C158617107 @default.
- W2158959097 hasConcept C158619295 @default.
- W2158959097 hasConcept C181199279 @default.
- W2158959097 hasConcept C204328495 @default.
- W2158959097 hasConcept C27740335 @default.
- W2158959097 hasConcept C2775962898 @default.
- W2158959097 hasConcept C47450691 @default.
- W2158959097 hasConcept C5098756 @default.
- W2158959097 hasConcept C55493867 @default.
- W2158959097 hasConcept C71924100 @default.
- W2158959097 hasConcept C86803240 @default.
- W2158959097 hasConcept C95444343 @default.
- W2158959097 hasConcept C98539663 @default.
- W2158959097 hasConceptScore W2158959097C12554922 @default.