FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2158976035> ?p ?o ?g. }

• W2158976035 endingPage "714" @default.
• W2158976035 startingPage "706" @default.
• W2158976035 abstract "With the aid of the recently developed Sensor reactor system, a series of three subsequent (13)C labeling experiments was performed mirroring the l-phenylalanine (l-Phe) production phase of a recombinant E. coli strain that was cultivated under industry-like conditions in a 300 L bioreactor. On the basis of the data from NMR labeling analysis, three subsequent flux patterns were successfully derived monitoring the l-Phe formation during an observation window from 14 to 23.3 h process time. Linear programming was performed to identify optimal flux patterns for l-Phe formation. Additionally, flux sensitivity analysis was used to identify the most promising metabolic engineering target. As a result, high rates of phosphoenolpyruvate (PEP) to pyruvate (PYR) conversion were identified as the most important reason for deterioration of the l-Phe/glucose yield from 20 to finally 11 mol %. Considering the characteristics of the enzyme kinetics involved, the working hypothesis was formulated that phosphoenolpyruvate synthase activity was increasingly hampered by rising oxaloacetate and 2-oxoglutarate concentrations, while at the same time pyruvate kinase activity arose due to activation by fructose 1,6-diphosphate. Hence, pps overexpression should be performed to optimize the existing production strain." @default.
• W2158976035 created "2016-06-24" @default.
• W2158976035 creator A5013662450 @default.
• W2158976035 creator A5018520418 @default.
• W2158976035 creator A5050132096 @default.
• W2158976035 creator A5079474407 @default.
• W2158976035 creator A5081992425 @default.
• W2158976035 date "2004-06-04" @default.
• W2158976035 modified "2023-10-10" @default.
• W2158976035 title "Serial 13C-Based Flux Analysis of an L-Phenylalanine-Producing E. coli Strain Using the Sensor Reactor" @default.
• W2158976035 cites W1504699676 @default.
• W2158976035 cites W1578261346 @default.
• W2158976035 cites W1585571745 @default.
• W2158976035 cites W1652052270 @default.
• W2158976035 cites W1973244479 @default.
• W2158976035 cites W1988431706 @default.
• W2158976035 cites W1990512751 @default.
• W2158976035 cites W2000487125 @default.
• W2158976035 cites W2004033922 @default.
• W2158976035 cites W2004446075 @default.
• W2158976035 cites W2004660097 @default.
• W2158976035 cites W2008818443 @default.
• W2158976035 cites W2011072729 @default.
• W2158976035 cites W2012493190 @default.
• W2158976035 cites W2015124166 @default.
• W2158976035 cites W2029491068 @default.
• W2158976035 cites W2038431430 @default.
• W2158976035 cites W2044318184 @default.
• W2158976035 cites W2048374178 @default.
• W2158976035 cites W2050916001 @default.
• W2158976035 cites W2051468192 @default.
• W2158976035 cites W2054744611 @default.
• W2158976035 cites W2059902696 @default.
• W2158976035 cites W2063490591 @default.
• W2158976035 cites W2084056034 @default.
• W2158976035 cites W2101752169 @default.
• W2158976035 cites W2140299962 @default.
• W2158976035 cites W2142570652 @default.
• W2158976035 cites W2148063387 @default.
• W2158976035 cites W2510052902 @default.
• W2158976035 cites W2952526948 @default.
• W2158976035 doi "https://doi.org/10.1021/bp0342755" @default.
• W2158976035 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15176872" @default.
• W2158976035 hasPublicationYear "2004" @default.
• W2158976035 type Work @default.
• W2158976035 sameAs 2158976035 @default.
• W2158976035 citedByCount "34" @default.
• W2158976035 countsByYear W21589760352013 @default.
• W2158976035 countsByYear W21589760352015 @default.
• W2158976035 countsByYear W21589760352016 @default.
• W2158976035 countsByYear W21589760352017 @default.
• W2158976035 crossrefType "journal-article" @default.
• W2158976035 hasAuthorship W2158976035A5013662450 @default.
• W2158976035 hasAuthorship W2158976035A5018520418 @default.
• W2158976035 hasAuthorship W2158976035A5050132096 @default.
• W2158976035 hasAuthorship W2158976035A5079474407 @default.
• W2158976035 hasAuthorship W2158976035A5081992425 @default.
• W2158976035 hasBestOaLocation W21589760352 @default.
• W2158976035 hasConcept C105702510 @default.
• W2158976035 hasConcept C111133373 @default.
• W2158976035 hasConcept C121332964 @default.
• W2158976035 hasConcept C143937172 @default.
• W2158976035 hasConcept C148898269 @default.
• W2158976035 hasConcept C160941953 @default.
• W2158976035 hasConcept C167091322 @default.
• W2158976035 hasConcept C168170006 @default.
• W2158976035 hasConcept C178790620 @default.
• W2158976035 hasConcept C181199279 @default.
• W2158976035 hasConcept C185592680 @default.
• W2158976035 hasConcept C20251656 @default.
• W2158976035 hasConcept C2777344606 @default.
• W2158976035 hasConcept C2778022156 @default.
• W2158976035 hasConcept C2780854706 @default.
• W2158976035 hasConcept C2781281423 @default.
• W2158976035 hasConcept C55493867 @default.
• W2158976035 hasConcept C62231903 @default.
• W2158976035 hasConcept C62520636 @default.
• W2158976035 hasConcept C6350086 @default.
• W2158976035 hasConcept C68709404 @default.
• W2158976035 hasConcept C86803240 @default.
• W2158976035 hasConceptScore W2158976035C105702510 @default.
• W2158976035 hasConceptScore W2158976035C111133373 @default.
• W2158976035 hasConceptScore W2158976035C121332964 @default.
• W2158976035 hasConceptScore W2158976035C143937172 @default.
• W2158976035 hasConceptScore W2158976035C148898269 @default.
• W2158976035 hasConceptScore W2158976035C160941953 @default.
• W2158976035 hasConceptScore W2158976035C167091322 @default.
• W2158976035 hasConceptScore W2158976035C168170006 @default.
• W2158976035 hasConceptScore W2158976035C178790620 @default.
• W2158976035 hasConceptScore W2158976035C181199279 @default.
• W2158976035 hasConceptScore W2158976035C185592680 @default.
• W2158976035 hasConceptScore W2158976035C20251656 @default.
• W2158976035 hasConceptScore W2158976035C2777344606 @default.
• W2158976035 hasConceptScore W2158976035C2778022156 @default.
• W2158976035 hasConceptScore W2158976035C2780854706 @default.
• W2158976035 hasConceptScore W2158976035C2781281423 @default.
• W2158976035 hasConceptScore W2158976035C55493867 @default.
• W2158976035 hasConceptScore W2158976035C62231903 @default.

• next