FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159048245> ?p ?o ?g. }

• W2159048245 endingPage "3574" @default.
• W2159048245 startingPage "3555" @default.
• W2159048245 abstract "The Kv2.1 voltage-gated K+ channel is widely expressed throughout mammalian brain, where it contributes to dynamic activity-dependent regulation of intrinsic neuronal excitability. Here we show that somatic plasma membrane Kv2.1 clusters are juxtaposed to clusters of intracellular ryanodine receptor (RyR) Ca2+ -release channels in mouse brain neurons, most prominently in medium spiny neurons (MSNs) of the striatum. Electron microscopy-immunogold labeling shows that in MSNs, plasma membrane Kv2.1 clusters are adjacent to subsurface cisternae, placing Kv2.1 in close proximity to sites of RyR-mediated Ca2+ release. Immunofluorescence labeling in transgenic mice expressing green fluorescent protein in specific MSN populations reveals the most prominent juxtaposed Kv2.1:RyR clusters in indirect pathway MSNs. Kv2.1 in both direct and indirect pathway MSNs exhibits markedly lower levels of labeling with phosphospecific antibodies directed against the S453, S563, and S603 phosphorylation site compared with levels observed in neocortical neurons, although labeling for Kv2.1 phosphorylation at S563 was significantly lower in indirect pathway MSNs compared with those in the direct pathway. Finally, acute stimulation of RyRs in heterologous cells causes a rapid hyperpolarizing shift in the voltage dependence of activation of Kv2.1, typical of Ca2+ /calcineurin-dependent Kv2.1 dephosphorylation. Together, these studies reveal that striatal MSNs are distinct in their expression of clustered Kv2.1 at plasma membrane sites juxtaposed to intracellular RyRs, as well as in Kv2.1 phosphorylation state. Differences in Kv2.1 expression and phosphorylation between MSNs in direct and indirect pathways provide a cell- and circuit-specific mechanism for coupling intracellular Ca2+ release to phosphorylation-dependent regulation of Kv2.1 to dynamically impact intrinsic excitability." @default.
• W2159048245 created "2016-06-24" @default.
• W2159048245 creator A5003417680 @default.
• W2159048245 creator A5047555131 @default.
• W2159048245 creator A5048615240 @default.
• W2159048245 creator A5051673875 @default.
• W2159048245 creator A5066448768 @default.
• W2159048245 creator A5090010687 @default.
• W2159048245 creator A5090270535 @default.
• W2159048245 date "2014-07-14" @default.
• W2159048245 modified "2023-10-17" @default.
• W2159048245 title "Cell type-specific spatial and functional coupling between mammalian brain Kv2.1 K⁺channels and ryanodine receptors" @default.
• W2159048245 cites W1515518093 @default.
• W2159048245 cites W1646051950 @default.
• W2159048245 cites W1900500057 @default.
• W2159048245 cites W1929540261 @default.
• W2159048245 cites W1970578796 @default.
• W2159048245 cites W1979106875 @default.
• W2159048245 cites W1981504301 @default.
• W2159048245 cites W1992969978 @default.
• W2159048245 cites W1994471332 @default.
• W2159048245 cites W1996589818 @default.
• W2159048245 cites W2003179114 @default.
• W2159048245 cites W2009697737 @default.
• W2159048245 cites W2012247789 @default.
• W2159048245 cites W2012458221 @default.
• W2159048245 cites W2017400430 @default.
• W2159048245 cites W2020388661 @default.
• W2159048245 cites W2020662075 @default.
• W2159048245 cites W2020887697 @default.
• W2159048245 cites W2023195612 @default.
• W2159048245 cites W2027047440 @default.
• W2159048245 cites W2031426087 @default.
• W2159048245 cites W2035721067 @default.
• W2159048245 cites W2036653918 @default.
• W2159048245 cites W2040682085 @default.
• W2159048245 cites W2041119907 @default.
• W2159048245 cites W2044606207 @default.
• W2159048245 cites W2046656676 @default.
• W2159048245 cites W2046902346 @default.
• W2159048245 cites W2055121607 @default.
• W2159048245 cites W2063256813 @default.
• W2159048245 cites W2065693120 @default.
• W2159048245 cites W2068401524 @default.
• W2159048245 cites W2068505134 @default.
• W2159048245 cites W2071651200 @default.
• W2159048245 cites W2072081603 @default.
• W2159048245 cites W2072471403 @default.
• W2159048245 cites W2073702803 @default.
• W2159048245 cites W2077081042 @default.
• W2159048245 cites W2079762354 @default.
• W2159048245 cites W2080633827 @default.
• W2159048245 cites W2086684841 @default.
• W2159048245 cites W2086998659 @default.
• W2159048245 cites W2088093088 @default.
• W2159048245 cites W2088347460 @default.
• W2159048245 cites W2099949778 @default.
• W2159048245 cites W2105828748 @default.
• W2159048245 cites W2110925924 @default.
• W2159048245 cites W2118830830 @default.
• W2159048245 cites W2120282263 @default.
• W2159048245 cites W2122916910 @default.
• W2159048245 cites W2132703716 @default.
• W2159048245 cites W2140875370 @default.
• W2159048245 cites W2144816990 @default.
• W2159048245 cites W2148694929 @default.
• W2159048245 cites W2151942667 @default.
• W2159048245 cites W2153863849 @default.
• W2159048245 cites W2162478231 @default.
• W2159048245 cites W2165647350 @default.
• W2159048245 cites W2170823110 @default.
• W2159048245 cites W4295332810 @default.
• W2159048245 doi "https://doi.org/10.1002/cne.23641" @default.
• W2159048245 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4139460" @default.
• W2159048245 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24962901" @default.
• W2159048245 hasPublicationYear "2014" @default.
• W2159048245 type Work @default.
• W2159048245 sameAs 2159048245 @default.
• W2159048245 citedByCount "54" @default.
• W2159048245 countsByYear W21590482452015 @default.
• W2159048245 countsByYear W21590482452016 @default.
• W2159048245 countsByYear W21590482452017 @default.
• W2159048245 countsByYear W21590482452018 @default.
• W2159048245 countsByYear W21590482452019 @default.
• W2159048245 countsByYear W21590482452020 @default.
• W2159048245 countsByYear W21590482452021 @default.
• W2159048245 countsByYear W21590482452022 @default.
• W2159048245 countsByYear W21590482452023 @default.
• W2159048245 crossrefType "journal-article" @default.
• W2159048245 hasAuthorship W2159048245A5003417680 @default.
• W2159048245 hasAuthorship W2159048245A5047555131 @default.
• W2159048245 hasAuthorship W2159048245A5048615240 @default.
• W2159048245 hasAuthorship W2159048245A5051673875 @default.
• W2159048245 hasAuthorship W2159048245A5066448768 @default.
• W2159048245 hasAuthorship W2159048245A5090010687 @default.
• W2159048245 hasAuthorship W2159048245A5090270535 @default.
• W2159048245 hasBestOaLocation W21590482452 @default.
• W2159048245 hasConcept C113217602 @default.

• next