Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159096946> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2159096946 endingPage "345" @default.
- W2159096946 startingPage "341" @default.
- W2159096946 abstract "The aim of the present investigation was to study the effect of oocyte selection on the efficiency of bovine nuclear transfer in terms of increased blastocyst production. For this purpose, prior to in vitro maturation (IVM), oocytes were selected for their developmental competence on the basis of glucose-6-phosphate dehydrogenase (G6PDH) activity indicated by brilliant cresyl blue (BCB) staining. It has been hypothesized that growing oocytes have a higher level of active G6PDH in comparison to the mature oocytes. Compact cumulus oocyte complexes (COCs) were recovered from slaughterhouse-collected bovine ovaries and classified either as control group, which were placed immediately into culture without exposure to BCB stain, or treatment group, which were stained with BCB for 90min before culture. Treated oocytes were then divided into BCB- (colourless cytoplasm, increased G6PDH) and BCB+ (coloured cytoplasm, low G6PDH) based on their ability to metabolize the stain. After IVM, oocytes were subjected to nuclear transfer procedure for the production of cloned embryos which were then cultured for a period of 8 days to determine the blastocyst rate. The BCB+ oocytes yielded a significantly higher blastocyst rate (39%) than the control (21%) or BCB- oocytes (4%). These results show that the staining of bovine cumulus-oocyte complexes with BCB before in vitro maturation could be used to select developmentally competent oocytes for nuclear transfer. In addition, G6PDH activity could prove to be a useful marker for determining the oocyte quality in future." @default.
- W2159096946 created "2016-06-24" @default.
- W2159096946 creator A5005513798 @default.
- W2159096946 creator A5036093736 @default.
- W2159096946 creator A5041577999 @default.
- W2159096946 creator A5053699950 @default.
- W2159096946 creator A5071113774 @default.
- W2159096946 date "2007-01-01" @default.
- W2159096946 modified "2023-10-18" @default.
- W2159096946 title "Selection of developmentally competent oocytes through brilliant cresyl blue stain enhances blastocyst development rate after bovine nuclear transfer" @default.
- W2159096946 cites W1979022809 @default.
- W2159096946 cites W1993764803 @default.
- W2159096946 cites W1997290700 @default.
- W2159096946 cites W2003517084 @default.
- W2159096946 cites W2019754734 @default.
- W2159096946 cites W2028981366 @default.
- W2159096946 cites W2041019830 @default.
- W2159096946 cites W2050587143 @default.
- W2159096946 cites W2057661574 @default.
- W2159096946 cites W2064890605 @default.
- W2159096946 cites W2087019844 @default.
- W2159096946 cites W2089726981 @default.
- W2159096946 cites W2103214426 @default.
- W2159096946 cites W2121151812 @default.
- W2159096946 cites W2124078316 @default.
- W2159096946 doi "https://doi.org/10.1016/j.theriogenology.2006.08.006" @default.
- W2159096946 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16999988" @default.
- W2159096946 hasPublicationYear "2007" @default.
- W2159096946 type Work @default.
- W2159096946 sameAs 2159096946 @default.
- W2159096946 citedByCount "81" @default.
- W2159096946 countsByYear W21590969462012 @default.
- W2159096946 countsByYear W21590969462013 @default.
- W2159096946 countsByYear W21590969462014 @default.
- W2159096946 countsByYear W21590969462015 @default.
- W2159096946 countsByYear W21590969462016 @default.
- W2159096946 countsByYear W21590969462017 @default.
- W2159096946 countsByYear W21590969462018 @default.
- W2159096946 countsByYear W21590969462019 @default.
- W2159096946 countsByYear W21590969462020 @default.
- W2159096946 countsByYear W21590969462021 @default.
- W2159096946 countsByYear W21590969462022 @default.
- W2159096946 countsByYear W21590969462023 @default.
- W2159096946 crossrefType "journal-article" @default.
- W2159096946 hasAuthorship W2159096946A5005513798 @default.
- W2159096946 hasAuthorship W2159096946A5036093736 @default.
- W2159096946 hasAuthorship W2159096946A5041577999 @default.
- W2159096946 hasAuthorship W2159096946A5053699950 @default.
- W2159096946 hasAuthorship W2159096946A5071113774 @default.
- W2159096946 hasConcept C16685009 @default.
- W2159096946 hasConcept C173372395 @default.
- W2159096946 hasConcept C185592680 @default.
- W2159096946 hasConcept C196843134 @default.
- W2159096946 hasConcept C2775966511 @default.
- W2159096946 hasConcept C2776690073 @default.
- W2159096946 hasConcept C2778177303 @default.
- W2159096946 hasConcept C54355233 @default.
- W2159096946 hasConcept C71924100 @default.
- W2159096946 hasConcept C74864618 @default.
- W2159096946 hasConcept C86803240 @default.
- W2159096946 hasConcept C87073359 @default.
- W2159096946 hasConcept C95444343 @default.
- W2159096946 hasConceptScore W2159096946C16685009 @default.
- W2159096946 hasConceptScore W2159096946C173372395 @default.
- W2159096946 hasConceptScore W2159096946C185592680 @default.
- W2159096946 hasConceptScore W2159096946C196843134 @default.
- W2159096946 hasConceptScore W2159096946C2775966511 @default.
- W2159096946 hasConceptScore W2159096946C2776690073 @default.
- W2159096946 hasConceptScore W2159096946C2778177303 @default.
- W2159096946 hasConceptScore W2159096946C54355233 @default.
- W2159096946 hasConceptScore W2159096946C71924100 @default.
- W2159096946 hasConceptScore W2159096946C74864618 @default.
- W2159096946 hasConceptScore W2159096946C86803240 @default.
- W2159096946 hasConceptScore W2159096946C87073359 @default.
- W2159096946 hasConceptScore W2159096946C95444343 @default.
- W2159096946 hasIssue "2" @default.
- W2159096946 hasLocation W21590969461 @default.
- W2159096946 hasLocation W21590969462 @default.
- W2159096946 hasOpenAccess W2159096946 @default.
- W2159096946 hasPrimaryLocation W21590969461 @default.
- W2159096946 hasRelatedWork W2050587143 @default.
- W2159096946 hasRelatedWork W2053605246 @default.
- W2159096946 hasRelatedWork W2150301254 @default.
- W2159096946 hasRelatedWork W2159096946 @default.
- W2159096946 hasRelatedWork W2186830054 @default.
- W2159096946 hasRelatedWork W2195810901 @default.
- W2159096946 hasRelatedWork W2349296746 @default.
- W2159096946 hasRelatedWork W2364870984 @default.
- W2159096946 hasRelatedWork W2617150033 @default.
- W2159096946 hasRelatedWork W4379599493 @default.
- W2159096946 hasVolume "67" @default.
- W2159096946 isParatext "false" @default.
- W2159096946 isRetracted "false" @default.
- W2159096946 magId "2159096946" @default.
- W2159096946 workType "article" @default.