FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159098596> ?p ?o ?g. }

• W2159098596 endingPage "1596" @default.
• W2159098596 startingPage "1591" @default.
• W2159098596 abstract "The glomerular epithelial polyanion is a specialized cell surface component found on renal glomerular epithelial cells (podocytes) that is rich in sialoprotein(s), as detected by staining with cationic dyes (colloidal iron, alcian blue) and wheat germ agglutinin (WGA). We have isolated rat glomeruli and analyzed their protein composition by SDS PAGE in 5-10% gradient gels. When the gels were stained with alcian blue or Stains All, a single band with an apparent Mr of 140,000 was detected that also stained very prominently with silver, but not with Coomassie Blue. This band predominated in fluorograms of gels of isolated glomeruli that had been labeled in their sialic acid residues by periodate-[3H]borohydride. In lectin overlays, the 140-kilodalton (kd) band was virtually the only one that bound [125I]wheat germ agglutinin, and this binding could be prevented by predigestion with neuraminidase. [125I]Peanut lectin bound exclusively to the 140-kd band after neuraminidase treatment. An antibody was prepared that specifically recognizes only the 140-kd band by immunoprecipitation and immuneoverlay. By immunoperoxidase and immunogold techniques, it was localized to the surface coat of the glomerular epithelium and, less extensively, to that of endothelial cells. When analyzed (after electroelution from preparative SDS gels), the 140-kd band was found to contain approximately 20% hexose and approximately 4.5% sialic acid. These findings indicate that the 140-kd protein is the major sialoprotein of the glomerulus, and it is the only component of glomerular lysates with an affinity for cationic dyes and lectins identical to that defined histochemically for the epithelial polyanion in situ. Since this molecule is a major component of the cell coat or glycocalyx of the podocytes, we have called it podocalyxin." @default.
• W2159098596 created "2016-06-24" @default.
• W2159098596 creator A5008619711 @default.
• W2159098596 creator A5077039235 @default.
• W2159098596 creator A5081438709 @default.
• W2159098596 date "1984-04-01" @default.
• W2159098596 modified "2023-10-16" @default.
• W2159098596 title "Identification and characterization of podocalyxin--the major sialoprotein of the renal glomerular epithelial cell." @default.
• W2159098596 cites W1520029826 @default.
• W2159098596 cites W1580023435 @default.
• W2159098596 cites W1619880965 @default.
• W2159098596 cites W168223360 @default.
• W2159098596 cites W170348712 @default.
• W2159098596 cites W1775749144 @default.
• W2159098596 cites W1893651774 @default.
• W2159098596 cites W1967769860 @default.
• W2159098596 cites W1976941367 @default.
• W2159098596 cites W1996071792 @default.
• W2159098596 cites W2001297687 @default.
• W2159098596 cites W2007522859 @default.
• W2159098596 cites W2007634300 @default.
• W2159098596 cites W2011403137 @default.
• W2159098596 cites W2018289835 @default.
• W2159098596 cites W2023271648 @default.
• W2159098596 cites W2030812496 @default.
• W2159098596 cites W2031878150 @default.
• W2159098596 cites W2037059785 @default.
• W2159098596 cites W2037815757 @default.
• W2159098596 cites W2049631138 @default.
• W2159098596 cites W2057955942 @default.
• W2159098596 cites W2060954256 @default.
• W2159098596 cites W2061677382 @default.
• W2159098596 cites W2064386269 @default.
• W2159098596 cites W2072838150 @default.
• W2159098596 cites W2075139124 @default.
• W2159098596 cites W2080676657 @default.
• W2159098596 cites W2087298783 @default.
• W2159098596 cites W2087949876 @default.
• W2159098596 cites W2095521299 @default.
• W2159098596 cites W2101108802 @default.
• W2159098596 cites W2113357723 @default.
• W2159098596 cites W2122148108 @default.
• W2159098596 cites W2131904026 @default.
• W2159098596 cites W2138874814 @default.
• W2159098596 cites W2397267740 @default.
• W2159098596 cites W2414342964 @default.
• W2159098596 cites W2415788628 @default.
• W2159098596 cites W2417396359 @default.
• W2159098596 cites W2418630866 @default.
• W2159098596 cites W2418981882 @default.
• W2159098596 cites W4235671636 @default.
• W2159098596 doi "https://doi.org/10.1083/jcb.98.4.1591" @default.
• W2159098596 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2113206" @default.
• W2159098596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6371025" @default.
• W2159098596 hasPublicationYear "1984" @default.
• W2159098596 type Work @default.
• W2159098596 sameAs 2159098596 @default.
• W2159098596 citedByCount "476" @default.
• W2159098596 countsByYear W21590985962012 @default.
• W2159098596 countsByYear W21590985962013 @default.
• W2159098596 countsByYear W21590985962014 @default.
• W2159098596 countsByYear W21590985962015 @default.
• W2159098596 countsByYear W21590985962016 @default.
• W2159098596 countsByYear W21590985962017 @default.
• W2159098596 countsByYear W21590985962018 @default.
• W2159098596 countsByYear W21590985962019 @default.
• W2159098596 countsByYear W21590985962020 @default.
• W2159098596 countsByYear W21590985962021 @default.
• W2159098596 countsByYear W21590985962022 @default.
• W2159098596 countsByYear W21590985962023 @default.
• W2159098596 crossrefType "journal-article" @default.
• W2159098596 hasAuthorship W2159098596A5008619711 @default.
• W2159098596 hasAuthorship W2159098596A5077039235 @default.
• W2159098596 hasAuthorship W2159098596A5081438709 @default.
• W2159098596 hasBestOaLocation W21590985961 @default.
• W2159098596 hasConcept C131075544 @default.
• W2159098596 hasConcept C134018914 @default.
• W2159098596 hasConcept C134164806 @default.
• W2159098596 hasConcept C153911025 @default.
• W2159098596 hasConcept C159654299 @default.
• W2159098596 hasConcept C160160445 @default.
• W2159098596 hasConcept C181199279 @default.
• W2159098596 hasConcept C203014093 @default.
• W2159098596 hasConcept C2776178622 @default.
• W2159098596 hasConcept C2776432469 @default.
• W2159098596 hasConcept C2777146197 @default.
• W2159098596 hasConcept C2777626840 @default.
• W2159098596 hasConcept C2779561371 @default.
• W2159098596 hasConcept C2780091579 @default.
• W2159098596 hasConcept C2780115458 @default.
• W2159098596 hasConcept C2780175098 @default.
• W2159098596 hasConcept C2780758401 @default.
• W2159098596 hasConcept C2781156571 @default.
• W2159098596 hasConcept C30030713 @default.
• W2159098596 hasConcept C55493867 @default.
• W2159098596 hasConcept C86803240 @default.
• W2159098596 hasConceptScore W2159098596C131075544 @default.
• W2159098596 hasConceptScore W2159098596C134018914 @default.

• next