FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159133774> ?p ?o ?g. }

• W2159133774 abstract "Abstract Background Cigarette smoke-induced cellular and molecular mechanisms of lung injury are not clear. Cigarette smoke is a complex mixture containing long-lived radicals, including p-benzosemiquinone that causes oxidative damage. Earlier we had reported that oxidative protein damage is an initial event in smoke-induced lung injury. Considering that p-benzosemiquinone may be a causative factor of lung injury, we have isolated p-benzosemiquinone and compared its pathophysiological effects with cigarette smoke. Since vitamin C is a strong antioxidant, we have also determined the modulatory effect of vitamin C for preventing the pathophysiological events. Methods Vitamin C-restricted guinea pigs were exposed to cigarette smoke (5 cigarettes/day; 2 puffs/cigarette) for 21 days with and without supplementation of 15 mg vitamin C/guinea pig/day. Oxidative damage, apoptosis and lung injury were assessed in vitro , ex vivo in A549 cells as well as in vivo in guinea pigs. Inflammation was measured by neutrophilia in BALF. p-Benzosemiquinone was isolated from freshly prepared aqueous extract of cigarette smoke and characterized by various physico-chemical methods, including mass, NMR and ESR spectroscopy. p-Benzosemiquinone-induced lung damage was examined by intratracheal instillation in guinea pigs. Lung damage was measured by increased air spaces, as evidenced by histology and morphometric analysis. Oxidative protein damage, MMPs, VEGF and VEGFR2 were measured by western blot analysis, and formation of Michael adducts using MALDI-TOF-MS. Apoptosis was evidenced by TUNEL assay, activation of caspase 3, degradation of PARP and increased Bax/Bcl-2 ratio using immunoblot analysis and confocal microscopy. Results Exposure of guinea pigs to cigarette smoke resulted in progressive protein damage, inflammation, apoptosis and lung injury up to 21 days of the experimental period. Administration of 15 mg of vitamin C/guinea pig/day prevented all these pathophysiological effects. p-Benzosemiquinone mimicked cigarette smoke in causing protein modification and apoptosis in vitro and in A549 cells ex vivo as well as apoptosis and lung damage in vivo . All these pathophysiological events were also prevented by vitamin C. Conclusion p-Benzosemiquinone appears to be a major causative factor of cigarette smoke-induced oxidative protein damage that leads to apoptosis and lung injury. The pathophysiological events are prevented by a moderately large dose of vitamin C." @default.
• W2159133774 created "2016-06-24" @default.
• W2159133774 creator A5006907567 @default.
• W2159133774 creator A5010064127 @default.
• W2159133774 creator A5025355304 @default.
• W2159133774 creator A5046416885 @default.
• W2159133774 creator A5050114481 @default.
• W2159133774 creator A5070409772 @default.
• W2159133774 creator A5070616314 @default.
• W2159133774 creator A5079665223 @default.
• W2159133774 date "2008-11-11" @default.
• W2159133774 modified "2023-10-15" @default.
• W2159133774 title "Cellular and molecular mechanisms of cigarette smoke-induced lung damage and prevention by vitamin C" @default.
• W2159133774 cites W119247573 @default.
• W2159133774 cites W1481859197 @default.
• W2159133774 cites W1508162082 @default.
• W2159133774 cites W1566407724 @default.
• W2159133774 cites W1581077481 @default.
• W2159133774 cites W1745634660 @default.
• W2159133774 cites W1747867097 @default.
• W2159133774 cites W1870141733 @default.
• W2159133774 cites W189427638 @default.
• W2159133774 cites W1908409619 @default.
• W2159133774 cites W1960975235 @default.
• W2159133774 cites W1971592740 @default.
• W2159133774 cites W1971909077 @default.
• W2159133774 cites W1975929558 @default.
• W2159133774 cites W1980761600 @default.
• W2159133774 cites W1995074959 @default.
• W2159133774 cites W1995980300 @default.
• W2159133774 cites W1996460415 @default.
• W2159133774 cites W2005589576 @default.
• W2159133774 cites W2008646918 @default.
• W2159133774 cites W2013319326 @default.
• W2159133774 cites W2019065245 @default.
• W2159133774 cites W2021302617 @default.
• W2159133774 cites W2027660719 @default.
• W2159133774 cites W2037393746 @default.
• W2159133774 cites W2042276081 @default.
• W2159133774 cites W2050477129 @default.
• W2159133774 cites W2051258629 @default.
• W2159133774 cites W2052793452 @default.
• W2159133774 cites W2054909375 @default.
• W2159133774 cites W2055446812 @default.
• W2159133774 cites W2061497413 @default.
• W2159133774 cites W2067675711 @default.
• W2159133774 cites W2071392303 @default.
• W2159133774 cites W2079293612 @default.
• W2159133774 cites W2090134402 @default.
• W2159133774 cites W2091864583 @default.
• W2159133774 cites W2101182707 @default.
• W2159133774 cites W2105064034 @default.
• W2159133774 cites W2113558571 @default.
• W2159133774 cites W2118197762 @default.
• W2159133774 cites W2118749829 @default.
• W2159133774 cites W2119630077 @default.
• W2159133774 cites W2119845801 @default.
• W2159133774 cites W2124631075 @default.
• W2159133774 cites W2128571455 @default.
• W2159133774 cites W2140521378 @default.
• W2159133774 cites W2144897212 @default.
• W2159133774 cites W2154239113 @default.
• W2159133774 cites W2154431017 @default.
• W2159133774 cites W2155134674 @default.
• W2159133774 cites W2159536730 @default.
• W2159133774 cites W2164754954 @default.
• W2159133774 cites W4214481755 @default.
• W2159133774 cites W89852714 @default.
• W2159133774 doi "https://doi.org/10.1186/1476-9255-5-21" @default.
• W2159133774 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2615750" @default.
• W2159133774 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19014449" @default.
• W2159133774 hasPublicationYear "2008" @default.
• W2159133774 type Work @default.
• W2159133774 sameAs 2159133774 @default.
• W2159133774 citedByCount "46" @default.
• W2159133774 countsByYear W21591337742012 @default.
• W2159133774 countsByYear W21591337742013 @default.
• W2159133774 countsByYear W21591337742014 @default.
• W2159133774 countsByYear W21591337742015 @default.
• W2159133774 countsByYear W21591337742016 @default.
• W2159133774 countsByYear W21591337742017 @default.
• W2159133774 countsByYear W21591337742018 @default.
• W2159133774 countsByYear W21591337742019 @default.
• W2159133774 countsByYear W21591337742021 @default.
• W2159133774 countsByYear W21591337742023 @default.
• W2159133774 crossrefType "journal-article" @default.
• W2159133774 hasAuthorship W2159133774A5006907567 @default.
• W2159133774 hasAuthorship W2159133774A5010064127 @default.
• W2159133774 hasAuthorship W2159133774A5025355304 @default.
• W2159133774 hasAuthorship W2159133774A5046416885 @default.
• W2159133774 hasAuthorship W2159133774A5050114481 @default.
• W2159133774 hasAuthorship W2159133774A5070409772 @default.
• W2159133774 hasAuthorship W2159133774A5070616314 @default.
• W2159133774 hasAuthorship W2159133774A5079665223 @default.
• W2159133774 hasBestOaLocation W21591337741 @default.
• W2159133774 hasConcept C126322002 @default.
• W2159133774 hasConcept C150903083 @default.
• W2159133774 hasConcept C16685009 @default.
• W2159133774 hasConcept C185592680 @default.
• W2159133774 hasConcept C190283241 @default.

• next