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• W2159161569 startingPage "1135" @default.
• W2159161569 abstract "Alternan, which consists of alternating alpha-(1-->3)/alpha-(1-->6)-linked glucosyl residues, was produced in potato tubers by expressing a mature alternansucrase (Asr) gene from Leuconostoc mesenteroides NRRL B-1355 in potato. Detection of alternan was performed by enzyme-linked immunosorbent assay in tuber juices, revealing a concentration between 0.3 and 1.2 mg g(-1) fresh wt. The Asr transcript levels correlated well with alternan accumulation in tuber juices. It appeared that the expression of sucrose-regulated starch-synthesizing genes (ADP-glucose pyrophosphorylase subunit S and granule-bound starch synthase I) was down-regulated. Despite this, the physico-chemical properties of the transgenic starches were unaltered. These results are compared to those obtained with other transgenic potato plants producing mutan [alpha-(1-->3)-linked glucosyl residues] and dextran [alpha-(1-->6)-linked glucosyl residues]." @default.
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• W2159161569 date "2007-03-23" @default.
• W2159161569 modified "2023-09-27" @default.
• W2159161569 title "Expression of alternansucrase in potato plants" @default.
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• W2159161569 doi "https://doi.org/10.1007/s10529-007-9348-z" @default.
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