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• W2159228510 endingPage "e93891" @default.
• W2159228510 startingPage "e93891" @default.
• W2159228510 abstract "In polyglutamine (polyQ) diseases including Huntington's disease (HD), mutant proteins containing expanded polyQ stretch form aggregates in neurons. Genetic or RNAi screenings in yeast, C. elegans or Drosophila have identified multiple genes modifying polyQ aggregation, a few of which are confirmed effective in mammals. However, the overall molecular mechanism underlying polyQ protein aggregation in mammalian cells still remains obscure. We here perform RNAi screening in mouse neuro2a cells to identify mammalian modifiers for aggregation of mutant huntingtin, a causative protein of HD. By systematic cell transfection and automated cell image analysis, we screen ∼12000 shRNA clones and identify 111 shRNAs that either suppress or enhance mutant huntingtin aggregation, without altering its gene expression. Classification of the shRNA-targets suggests that genes with various cellular functions such as gene transcription and protein phosphorylation are involved in modifying the aggregation. Subsequent analysis suggests that, in addition to the aggregation-modifiers sensitive to proteasome inhibition, some of them, such as a transcription factor Tcf20, and kinases Csnk1d and Pik3c2a, are insensitive to it. As for Tcf20, which contains polyQ stretches at N-terminus, its binding to mutant huntingtin aggregates is observed in neuro2a cells and in HD model mouse neurons. Notably, except Pik3c2a, the rest of the modifiers identified here are novel. Thus, our first large-scale RNAi screening in mammalian system identifies previously undescribed genetic players that regulate mutant huntingtin aggregation by several, possibly mammalian-specific mechanisms." @default.
• W2159228510 created "2016-06-24" @default.
• W2159228510 creator A5009883805 @default.
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• W2159228510 creator A5079026548 @default.
• W2159228510 creator A5081269409 @default.
• W2159228510 date "2014-04-04" @default.
• W2159228510 modified "2023-10-16" @default.
• W2159228510 title "Large-Scale RNA Interference Screening in Mammalian Cells Identifies Novel Regulators of Mutant Huntingtin Aggregation" @default.
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• W2159228510 doi "https://doi.org/10.1371/journal.pone.0093891" @default.
• W2159228510 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3976342" @default.
• W2159228510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24705917" @default.
• W2159228510 hasPublicationYear "2014" @default.
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