FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159310265> ?p ?o ?g. }

• W2159310265 endingPage "125" @default.
• W2159310265 startingPage "119" @default.
• W2159310265 abstract "ABSTRACT To explore the structural basis for the essential role of calmodulin (CaM) in Aspergillus nidulans, we have compared the biochemical and in vivo properties of A. nidulans CaM (AnCaM) with those of heterologous CaMs. Neither Saccharomyces cerevisiae CaM (ScCaM) nor a Ca 2+ binding mutant of A. nidulans CaM (1234) interacts appreciably with A. nidulans CaM binding proteins by an overlay assay or activates two essential CaMKs, CMKA and CMKB. In contrast, although vertebrate CaM (VCaM) binds a spectrum of proteins similar to that for AnCaM, it is unable to fully activate CMKA and CMKB, displaying a higher K CaM and reduced V max for both enzymes. In correlation with the biochemical analysis, neither ScCaM nor 1234 can support A. nidulans growth in the absence of the endogenous protein, whereas VCaM only partially complements the absence of wild-type CaM. Analysis of VCaM and AnCaM chimeras demonstrates that amino acid variations in both N- and C-terminal domains contribute to the inability of VCaM to activate CMKB, but differences in the N terminus are largely responsible for the reduced activity towards CMKA. In vivo, the chimeric molecules support growth equivalently, but only to levels intermediate between those of VCaM and AnCaM, suggesting that the reduced ability to activate the CaMKs is not solely responsible for the inability of VCaM to complement the absence of the wild-type protein. Thus, not only is Ca 2+ binding required for CaM function in A. nidulans, but the essential in vivo functions of A. nidulans CaM are uniquely sensitive to the subtle amino acid variations present in vertebrate CaM." @default.
• W2159310265 created "2016-06-24" @default.
• W2159310265 creator A5037379407 @default.
• W2159310265 creator A5085156788 @default.
• W2159310265 date "2002-02-01" @default.
• W2159310265 modified "2023-09-29" @default.
• W2159310265 title "Calcium Binding Is Required for Calmodulin Function in <i>Aspergillus nidulans</i>" @default.
• W2159310265 cites W11988849 @default.
• W2159310265 cites W1526906553 @default.
• W2159310265 cites W1548143133 @default.
• W2159310265 cites W1554025750 @default.
• W2159310265 cites W1555670473 @default.
• W2159310265 cites W1576403707 @default.
• W2159310265 cites W1587918801 @default.
• W2159310265 cites W1590715819 @default.
• W2159310265 cites W1810166674 @default.
• W2159310265 cites W1967407965 @default.
• W2159310265 cites W1967897161 @default.
• W2159310265 cites W1985339191 @default.
• W2159310265 cites W1987159249 @default.
• W2159310265 cites W1987312402 @default.
• W2159310265 cites W1994323526 @default.
• W2159310265 cites W2001535102 @default.
• W2159310265 cites W2012538055 @default.
• W2159310265 cites W2016968116 @default.
• W2159310265 cites W2024142216 @default.
• W2159310265 cites W2028724687 @default.
• W2159310265 cites W2031811477 @default.
• W2159310265 cites W2038156324 @default.
• W2159310265 cites W2046522192 @default.
• W2159310265 cites W2052366943 @default.
• W2159310265 cites W2053578572 @default.
• W2159310265 cites W2068651542 @default.
• W2159310265 cites W2075659345 @default.
• W2159310265 cites W2093695208 @default.
• W2159310265 cites W2093714495 @default.
• W2159310265 cites W2097283858 @default.
• W2159310265 cites W2137130856 @default.
• W2159310265 cites W2145954184 @default.
• W2159310265 cites W2147728137 @default.
• W2159310265 cites W2162294247 @default.
• W2159310265 cites W2165104370 @default.
• W2159310265 cites W2166626043 @default.
• W2159310265 cites W4237058325 @default.
• W2159310265 cites W4237247150 @default.
• W2159310265 cites W4256601442 @default.
• W2159310265 doi "https://doi.org/10.1128/ec.01.1.119-125.2002" @default.
• W2159310265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/118048" @default.
• W2159310265 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12455978" @default.
• W2159310265 hasPublicationYear "2002" @default.
• W2159310265 type Work @default.
• W2159310265 sameAs 2159310265 @default.
• W2159310265 citedByCount "24" @default.
• W2159310265 countsByYear W21593102652012 @default.
• W2159310265 countsByYear W21593102652014 @default.
• W2159310265 countsByYear W21593102652015 @default.
• W2159310265 countsByYear W21593102652016 @default.
• W2159310265 countsByYear W21593102652017 @default.
• W2159310265 countsByYear W21593102652020 @default.
• W2159310265 countsByYear W21593102652021 @default.
• W2159310265 countsByYear W21593102652022 @default.
• W2159310265 crossrefType "journal-article" @default.
• W2159310265 hasAuthorship W2159310265A5037379407 @default.
• W2159310265 hasAuthorship W2159310265A5085156788 @default.
• W2159310265 hasBestOaLocation W21593102652 @default.
• W2159310265 hasConcept C104317684 @default.
• W2159310265 hasConcept C143065580 @default.
• W2159310265 hasConcept C181199279 @default.
• W2159310265 hasConcept C2776923806 @default.
• W2159310265 hasConcept C2777576037 @default.
• W2159310265 hasConcept C29688787 @default.
• W2159310265 hasConcept C55493867 @default.
• W2159310265 hasConcept C86803240 @default.
• W2159310265 hasConcept C95444343 @default.
• W2159310265 hasConceptScore W2159310265C104317684 @default.
• W2159310265 hasConceptScore W2159310265C143065580 @default.
• W2159310265 hasConceptScore W2159310265C181199279 @default.
• W2159310265 hasConceptScore W2159310265C2776923806 @default.
• W2159310265 hasConceptScore W2159310265C2777576037 @default.
• W2159310265 hasConceptScore W2159310265C29688787 @default.
• W2159310265 hasConceptScore W2159310265C55493867 @default.
• W2159310265 hasConceptScore W2159310265C86803240 @default.
• W2159310265 hasConceptScore W2159310265C95444343 @default.
• W2159310265 hasIssue "1" @default.
• W2159310265 hasLocation W21593102651 @default.
• W2159310265 hasLocation W21593102652 @default.
• W2159310265 hasLocation W21593102653 @default.
• W2159310265 hasOpenAccess W2159310265 @default.
• W2159310265 hasPrimaryLocation W21593102651 @default.
• W2159310265 hasRelatedWork W1585843265 @default.
• W2159310265 hasRelatedWork W1968610335 @default.
• W2159310265 hasRelatedWork W2020817525 @default.
• W2159310265 hasRelatedWork W2041752304 @default.
• W2159310265 hasRelatedWork W2062922630 @default.
• W2159310265 hasRelatedWork W2066213978 @default.
• W2159310265 hasRelatedWork W2090416769 @default.
• W2159310265 hasRelatedWork W2143105995 @default.
• W2159310265 hasRelatedWork W2156674132 @default.

• next