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- W2159434057 abstract "Recent advances in site-specific gene editing technologies, split genetic systems, recombinases and transposon remobilization have been applied to disease vector mosquitoes with great success. These in turn now enable a suite of hypothesis-driven questions concerning the physiology and behaviour of important mosquito species, guided by previous advances in genome sequencing and transcriptomics. In this chapter, we will review progress in CRISPR/Cas9-based gene disruption, including target selection, mutation identification, tracking homology-dependent gene insertion, CRISPR-mediated gene drive mechanisms and CRISPR/Cas9 variants. We will also review progress in using split genetic systems such as Gal4/UAS, recombinase-mediated cassette exchange using ΦC31, as well as piggyBac-mediated forward genetic screens using transgene remobilization." @default.
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- W2159434057 date "2009-01-01" @default.
- W2159434057 modified "2023-09-27" @default.
- W2159434057 title "Visualization of functional neural circuits using the GAL4-UAS system in zebrafish" @default.
- W2159434057 doi "https://doi.org/10.1016/j.neures.2009.09.227" @default.
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