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- W2159484613 abstract "2-Aminopurine is a fluorescent probe widely used to study local dynamics as well as charge and energy transfer reactions in DNA/RNA. Despite its broad utilization, the nonradiative relaxation pathways responsible for the variation in its fluorescence quantum yield and fluorescence lifetime in different solvents are still under scrutiny. In this work we use steady-state absorption and emission spectroscopy and broad-band transient absorption covering the time scale from femtoseconds to microseconds to investigate the excited-state dynamics of 2-aminopurine 2’-deoxyriboside (2APdr) in acetonitrile, ethanol, and aqueous buffer solution at pH 7. It is shown that up to ≈40% of the initial excited-state population decays by intersystem crossing to the triplet state depending on the solvent used, thus competing effectively with fluorescence emission. Furthermore, the rate of formation and yield of the triplet state depend sensitively on the hydrogen-donor ability and polarity of the solvent." @default.
- W2159484613 created "2016-06-24" @default.
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- W2159484613 date "2013-08-01" @default.
- W2159484613 modified "2023-10-06" @default.
- W2159484613 title "Role of intersystem crossing in the fluorescence quenching of 2-aminopurine 2’-deoxyriboside in solution" @default.
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- W2159484613 doi "https://doi.org/10.1039/c3pp25437b" @default.
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