FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159494038> ?p ?o ?g. }

• W2159494038 endingPage "417" @default.
• W2159494038 startingPage "411" @default.
• W2159494038 abstract "Although tumor necrosis factor alpha (TNF-α) is known to play a critical role in intervertebral disc (IVD) degeneration, the effect of TNF-α on nucleus pulposus (NP) cells has not yet been elucidated. The aim of this study was to explore the effect of TNF-α on proliferation of human NP cells. NP cells were treated with different concentrations of TNF-α. Cell proliferation was determined by cell counting kit-8 (CCK-8) analysis and Ki67 immunofluorescence staining, and expression of cyclin B1 was studied by quantitative real-time RT-PCR. Cell cycle was measured by flow cytometry and cell apoptosis was analyzed using an Annexin V–fluorescein isothiocyanate (FITC) & propidium iodide (PI) apoptosis detection kit. To identify the mechanism by which TNF-α induced proliferation of NP cells, selective inhibitors of major signaling pathways were used and Western blotting was carried out. Treatment with TNF-α increased cell viability (as determined by CCK-8 analysis) and expression of cyclin B1 and the number of Ki67-positive and S-phase NP cells, indicating enhancement of proliferation. Consistent with this, NP cell apoptosis was suppressed by TNF-α treatment. Moreover, inhibition of NF-κB, c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK) blocked TNF-α-stimulated proliferation of NP cells. In conclusion, the current findings suggest that the effect of TNF-α on IVD degeneration involves promotion of the proliferation of human NP cells via the NF-κB, JNK, and p38 MAPK pathways." @default.
• W2159494038 created "2016-06-24" @default.
• W2159494038 creator A5003187898 @default.
• W2159494038 creator A5007661222 @default.
• W2159494038 creator A5020147975 @default.
• W2159494038 creator A5025565506 @default.
• W2159494038 creator A5051531257 @default.
• W2159494038 creator A5063998384 @default.
• W2159494038 creator A5065778850 @default.
• W2159494038 creator A5071229987 @default.
• W2159494038 date "2014-10-10" @default.
• W2159494038 modified "2023-09-26" @default.
• W2159494038 title "Tumor necrosis factor alpha promotes the proliferation of human nucleus pulposus cells via nuclear factor-κB, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase" @default.
• W2159494038 cites W1453797714 @default.
• W2159494038 cites W1492606093 @default.
• W2159494038 cites W1509596990 @default.
• W2159494038 cites W1578639156 @default.
• W2159494038 cites W1966782529 @default.
• W2159494038 cites W1967613174 @default.
• W2159494038 cites W1982072699 @default.
• W2159494038 cites W1983979356 @default.
• W2159494038 cites W1989731738 @default.
• W2159494038 cites W1994654873 @default.
• W2159494038 cites W1998968944 @default.
• W2159494038 cites W2019239719 @default.
• W2159494038 cites W2026908726 @default.
• W2159494038 cites W2028577534 @default.
• W2159494038 cites W2033036084 @default.
• W2159494038 cites W2038083756 @default.
• W2159494038 cites W2039176395 @default.
• W2159494038 cites W2048270768 @default.
• W2159494038 cites W2055395294 @default.
• W2159494038 cites W2057421194 @default.
• W2159494038 cites W2063582323 @default.
• W2159494038 cites W2066113694 @default.
• W2159494038 cites W2068547085 @default.
• W2159494038 cites W2068747348 @default.
• W2159494038 cites W2068890508 @default.
• W2159494038 cites W2069714642 @default.
• W2159494038 cites W2073864477 @default.
• W2159494038 cites W2081367726 @default.
• W2159494038 cites W2083704112 @default.
• W2159494038 cites W2086627361 @default.
• W2159494038 cites W2087770419 @default.
• W2159494038 cites W2089872423 @default.
• W2159494038 cites W2104082092 @default.
• W2159494038 cites W2104769989 @default.
• W2159494038 cites W2116437268 @default.
• W2159494038 cites W2142384246 @default.
• W2159494038 cites W2148308989 @default.
• W2159494038 cites W2158792989 @default.
• W2159494038 cites W2332298625 @default.
• W2159494038 cites W377849966 @default.
• W2159494038 cites W4230932862 @default.
• W2159494038 doi "https://doi.org/10.1177/1535370214554533" @default.
• W2159494038 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4935371" @default.
• W2159494038 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25304312" @default.
• W2159494038 hasPublicationYear "2014" @default.
• W2159494038 type Work @default.
• W2159494038 sameAs 2159494038 @default.
• W2159494038 citedByCount "36" @default.
• W2159494038 countsByYear W21594940382015 @default.
• W2159494038 countsByYear W21594940382016 @default.
• W2159494038 countsByYear W21594940382017 @default.
• W2159494038 countsByYear W21594940382018 @default.
• W2159494038 countsByYear W21594940382019 @default.
• W2159494038 countsByYear W21594940382020 @default.
• W2159494038 countsByYear W21594940382021 @default.
• W2159494038 countsByYear W21594940382022 @default.
• W2159494038 countsByYear W21594940382023 @default.
• W2159494038 crossrefType "journal-article" @default.
• W2159494038 hasAuthorship W2159494038A5003187898 @default.
• W2159494038 hasAuthorship W2159494038A5007661222 @default.
• W2159494038 hasAuthorship W2159494038A5020147975 @default.
• W2159494038 hasAuthorship W2159494038A5025565506 @default.
• W2159494038 hasAuthorship W2159494038A5051531257 @default.
• W2159494038 hasAuthorship W2159494038A5063998384 @default.
• W2159494038 hasAuthorship W2159494038A5065778850 @default.
• W2159494038 hasAuthorship W2159494038A5071229987 @default.
• W2159494038 hasBestOaLocation W21594940382 @default.
• W2159494038 hasConcept C153911025 @default.
• W2159494038 hasConcept C17991360 @default.
• W2159494038 hasConcept C184235292 @default.
• W2159494038 hasConcept C185592680 @default.
• W2159494038 hasConcept C190283241 @default.
• W2159494038 hasConcept C203014093 @default.
• W2159494038 hasConcept C2775934118 @default.
• W2159494038 hasConcept C29537977 @default.
• W2159494038 hasConcept C31573885 @default.
• W2159494038 hasConcept C51551487 @default.
• W2159494038 hasConcept C53227056 @default.
• W2159494038 hasConcept C55493867 @default.
• W2159494038 hasConcept C57074206 @default.
• W2159494038 hasConcept C62112901 @default.
• W2159494038 hasConcept C86803240 @default.
• W2159494038 hasConcept C95444343 @default.
• W2159494038 hasConceptScore W2159494038C153911025 @default.
• W2159494038 hasConceptScore W2159494038C17991360 @default.

• next