Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159564680> ?p ?o ?g. }
- W2159564680 endingPage "1147" @default.
- W2159564680 startingPage "1140" @default.
- W2159564680 abstract "We previously reported an epitope presenting vector, pCI, a derivative of a human invariant chain (Ii) expression vector, in which the class II associated invariant chain peptide (CLIP, Ii p89–101) could be substituted with antigenic peptides. In the current study, we used this vector to develop a new expression cloning system to identify CD4+ T cell epitopes. We inserted double-stranded oligo DNAs of randomized sequences into this vector and prepared an epitope-presenting library which loads randomized 13-mer peptides onto HLA class II molecules coexpressed in COS-7 cells. Utilizing this library, we isolated a cross-reactive epitope recognized by a glutamic acid decarboxylase (GAD) 65-autoreactive T cell clone established from a patient with insulin-dependent diabetes mellitus. Although the newly identified epitope (PVQLSNQWHVVGATF) was far different from the original epitope, GAD65 p116–128 (NILLQYVVKSFDR), it did have the capacity to stimulate the T cell clone comparable to that of the original GAD epitope. Our system may be applicable not only for identifying of cross-reactive epitopes for CD4+ T cells of known specificity, but also for detection of epitopes stimulatory for CD4+ T cells the epitopes of which are unknown." @default.
- W2159564680 created "2016-06-24" @default.
- W2159564680 creator A5001859263 @default.
- W2159564680 creator A5007957984 @default.
- W2159564680 creator A5027250563 @default.
- W2159564680 creator A5036243714 @default.
- W2159564680 creator A5062600228 @default.
- W2159564680 creator A5085127863 @default.
- W2159564680 date "2001-06-01" @default.
- W2159564680 modified "2023-10-10" @default.
- W2159564680 title "Establishment of an Expression Cloning System for CD4+ T Cell Epitopes" @default.
- W2159564680 cites W1509401982 @default.
- W2159564680 cites W1546143943 @default.
- W2159564680 cites W1554362052 @default.
- W2159564680 cites W1584478189 @default.
- W2159564680 cites W1845604847 @default.
- W2159564680 cites W1935822176 @default.
- W2159564680 cites W1955836577 @default.
- W2159564680 cites W1965546174 @default.
- W2159564680 cites W1966551297 @default.
- W2159564680 cites W1975663554 @default.
- W2159564680 cites W1986216579 @default.
- W2159564680 cites W1986730157 @default.
- W2159564680 cites W1990204841 @default.
- W2159564680 cites W1992196706 @default.
- W2159564680 cites W1994849653 @default.
- W2159564680 cites W1995201985 @default.
- W2159564680 cites W2009563773 @default.
- W2159564680 cites W2018027744 @default.
- W2159564680 cites W2037678783 @default.
- W2159564680 cites W2038108432 @default.
- W2159564680 cites W2045219246 @default.
- W2159564680 cites W2060264122 @default.
- W2159564680 cites W2062634915 @default.
- W2159564680 cites W2066844508 @default.
- W2159564680 cites W2081060219 @default.
- W2159564680 cites W2087106927 @default.
- W2159564680 cites W2095843724 @default.
- W2159564680 cites W2096290400 @default.
- W2159564680 cites W2104649518 @default.
- W2159564680 cites W2122268842 @default.
- W2159564680 cites W2126346113 @default.
- W2159564680 cites W2128184382 @default.
- W2159564680 cites W2129185631 @default.
- W2159564680 cites W2134364025 @default.
- W2159564680 cites W2139835112 @default.
- W2159564680 cites W2156448847 @default.
- W2159564680 cites W2161332782 @default.
- W2159564680 cites W2166827072 @default.
- W2159564680 cites W4313342418 @default.
- W2159564680 doi "https://doi.org/10.1006/bbrc.2001.5107" @default.
- W2159564680 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11414702" @default.
- W2159564680 hasPublicationYear "2001" @default.
- W2159564680 type Work @default.
- W2159564680 sameAs 2159564680 @default.
- W2159564680 citedByCount "8" @default.
- W2159564680 crossrefType "journal-article" @default.
- W2159564680 hasAuthorship W2159564680A5001859263 @default.
- W2159564680 hasAuthorship W2159564680A5007957984 @default.
- W2159564680 hasAuthorship W2159564680A5027250563 @default.
- W2159564680 hasAuthorship W2159564680A5036243714 @default.
- W2159564680 hasAuthorship W2159564680A5062600228 @default.
- W2159564680 hasAuthorship W2159564680A5085127863 @default.
- W2159564680 hasConcept C104317684 @default.
- W2159564680 hasConcept C147483822 @default.
- W2159564680 hasConcept C153911025 @default.
- W2159564680 hasConcept C185592680 @default.
- W2159564680 hasConcept C195616568 @default.
- W2159564680 hasConcept C203014093 @default.
- W2159564680 hasConcept C55493867 @default.
- W2159564680 hasConcept C78598654 @default.
- W2159564680 hasConcept C81089528 @default.
- W2159564680 hasConcept C86803240 @default.
- W2159564680 hasConceptScore W2159564680C104317684 @default.
- W2159564680 hasConceptScore W2159564680C147483822 @default.
- W2159564680 hasConceptScore W2159564680C153911025 @default.
- W2159564680 hasConceptScore W2159564680C185592680 @default.
- W2159564680 hasConceptScore W2159564680C195616568 @default.
- W2159564680 hasConceptScore W2159564680C203014093 @default.
- W2159564680 hasConceptScore W2159564680C55493867 @default.
- W2159564680 hasConceptScore W2159564680C78598654 @default.
- W2159564680 hasConceptScore W2159564680C81089528 @default.
- W2159564680 hasConceptScore W2159564680C86803240 @default.
- W2159564680 hasFunder F4320320912 @default.
- W2159564680 hasFunder F4320321945 @default.
- W2159564680 hasIssue "5" @default.
- W2159564680 hasLocation W21595646801 @default.
- W2159564680 hasLocation W21595646802 @default.
- W2159564680 hasOpenAccess W2159564680 @default.
- W2159564680 hasPrimaryLocation W21595646801 @default.
- W2159564680 hasRelatedWork W1991572753 @default.
- W2159564680 hasRelatedWork W1996102030 @default.
- W2159564680 hasRelatedWork W2003116223 @default.
- W2159564680 hasRelatedWork W2016943232 @default.
- W2159564680 hasRelatedWork W2029383042 @default.
- W2159564680 hasRelatedWork W2062700146 @default.
- W2159564680 hasRelatedWork W3096915742 @default.
- W2159564680 hasRelatedWork W3139969521 @default.