FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159569892> ?p ?o ?g. }

• W2159569892 endingPage "651" @default.
• W2159569892 startingPage "641" @default.
• W2159569892 abstract "Abstract Using zinc-finger nucleases (ZFNs) to cleave the chromosomal target, we have achieved high frequencies of gene targeting in the Drosophila germline. Both local mutagenesis through nonhomologous end joining (NHEJ) and gene replacement via homologous recombination (HR) are stimulated by target cleavage. In this study we investigated the mechanisms that underlie these processes, using materials for the rosy (ry) locus. The frequency of HR dropped significantly in flies homozygous for mutations in spnA (Rad51) or okr (Rad54), two components of the invasion-mediated synthesis-dependent strand annealing (SDSA) pathway. When single-strand annealing (SSA) was also blocked by the use of a circular donor DNA, HR was completely abolished. This indicates that the majority of HR proceeds via SDSA, with a minority mediated by SSA. In flies deficient in lig4 (DNA ligase IV), a component of the major NHEJ pathway, the proportion of HR products rose significantly. This indicates that most NHEJ products are produced in a lig4-dependent process. When both spnA and lig4 were mutated and a circular donor was provided, the frequency of ry mutations was still high and no HR products were recovered. The local mutations produced in these circumstances must have arisen through an alternative, lig4-independent end-joining mechanism. These results show what repair pathways operate on double-strand breaks in this gene targeting system. They also demonstrate that the outcome can be biased toward gene replacement by disabling the major NHEJ pathway and toward simple mutagenesis by interfering with the major HR process." @default.
• W2159569892 created "2016-06-24" @default.
• W2159569892 creator A5036390483 @default.
• W2159569892 creator A5045017594 @default.
• W2159569892 creator A5074041061 @default.
• W2159569892 creator A5087746555 @default.
• W2159569892 date "2009-07-01" @default.
• W2159569892 modified "2023-10-17" @default.
• W2159569892 title "Genetic Analysis of Zinc-Finger Nuclease-Induced Gene Targeting in Drosophila" @default.
• W2159569892 cites W1627029737 @default.
• W2159569892 cites W1983321902 @default.
• W2159569892 cites W2133432686 @default.
• W2159569892 cites W2140886363 @default.
• W2159569892 doi "https://doi.org/10.1534/genetics.109.101329" @default.
• W2159569892 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2710147" @default.
• W2159569892 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19380480" @default.
• W2159569892 hasPublicationYear "2009" @default.
• W2159569892 type Work @default.
• W2159569892 sameAs 2159569892 @default.
• W2159569892 citedByCount "105" @default.
• W2159569892 countsByYear W21595698922012 @default.
• W2159569892 countsByYear W21595698922013 @default.
• W2159569892 countsByYear W21595698922014 @default.
• W2159569892 countsByYear W21595698922015 @default.
• W2159569892 countsByYear W21595698922016 @default.
• W2159569892 countsByYear W21595698922017 @default.
• W2159569892 countsByYear W21595698922018 @default.
• W2159569892 countsByYear W21595698922019 @default.
• W2159569892 countsByYear W21595698922020 @default.
• W2159569892 countsByYear W21595698922021 @default.
• W2159569892 countsByYear W21595698922022 @default.
• W2159569892 countsByYear W21595698922023 @default.
• W2159569892 crossrefType "journal-article" @default.
• W2159569892 hasAuthorship W2159569892A5036390483 @default.
• W2159569892 hasAuthorship W2159569892A5045017594 @default.
• W2159569892 hasAuthorship W2159569892A5074041061 @default.
• W2159569892 hasAuthorship W2159569892A5087746555 @default.
• W2159569892 hasBestOaLocation W21595698921 @default.
• W2159569892 hasConcept C102744134 @default.
• W2159569892 hasConcept C104317684 @default.
• W2159569892 hasConcept C111829913 @default.
• W2159569892 hasConcept C122917832 @default.
• W2159569892 hasConcept C134935766 @default.
• W2159569892 hasConcept C144501496 @default.
• W2159569892 hasConcept C145803527 @default.
• W2159569892 hasConcept C153911025 @default.
• W2159569892 hasConcept C16318435 @default.
• W2159569892 hasConcept C175114707 @default.
• W2159569892 hasConcept C193104389 @default.
• W2159569892 hasConcept C2779012473 @default.
• W2159569892 hasConcept C35802184 @default.
• W2159569892 hasConcept C501734568 @default.
• W2159569892 hasConcept C54355233 @default.
• W2159569892 hasConcept C552990157 @default.
• W2159569892 hasConcept C60748783 @default.
• W2159569892 hasConcept C86339819 @default.
• W2159569892 hasConcept C86803240 @default.
• W2159569892 hasConcept C94966510 @default.
• W2159569892 hasConcept C98108389 @default.
• W2159569892 hasConceptScore W2159569892C102744134 @default.
• W2159569892 hasConceptScore W2159569892C104317684 @default.
• W2159569892 hasConceptScore W2159569892C111829913 @default.
• W2159569892 hasConceptScore W2159569892C122917832 @default.
• W2159569892 hasConceptScore W2159569892C134935766 @default.
• W2159569892 hasConceptScore W2159569892C144501496 @default.
• W2159569892 hasConceptScore W2159569892C145803527 @default.
• W2159569892 hasConceptScore W2159569892C153911025 @default.
• W2159569892 hasConceptScore W2159569892C16318435 @default.
• W2159569892 hasConceptScore W2159569892C175114707 @default.
• W2159569892 hasConceptScore W2159569892C193104389 @default.
• W2159569892 hasConceptScore W2159569892C2779012473 @default.
• W2159569892 hasConceptScore W2159569892C35802184 @default.
• W2159569892 hasConceptScore W2159569892C501734568 @default.
• W2159569892 hasConceptScore W2159569892C54355233 @default.
• W2159569892 hasConceptScore W2159569892C552990157 @default.
• W2159569892 hasConceptScore W2159569892C60748783 @default.
• W2159569892 hasConceptScore W2159569892C86339819 @default.
• W2159569892 hasConceptScore W2159569892C86803240 @default.
• W2159569892 hasConceptScore W2159569892C94966510 @default.
• W2159569892 hasConceptScore W2159569892C98108389 @default.
• W2159569892 hasIssue "3" @default.
• W2159569892 hasLocation W21595698921 @default.
• W2159569892 hasLocation W21595698922 @default.
• W2159569892 hasLocation W21595698923 @default.
• W2159569892 hasLocation W21595698924 @default.
• W2159569892 hasOpenAccess W2159569892 @default.
• W2159569892 hasPrimaryLocation W21595698921 @default.
• W2159569892 hasRelatedWork W1873396772 @default.
• W2159569892 hasRelatedWork W1981288489 @default.
• W2159569892 hasRelatedWork W1987237527 @default.
• W2159569892 hasRelatedWork W2007377687 @default.
• W2159569892 hasRelatedWork W2028803759 @default.
• W2159569892 hasRelatedWork W2088452936 @default.
• W2159569892 hasRelatedWork W2152416923 @default.
• W2159569892 hasRelatedWork W2159569892 @default.
• W2159569892 hasRelatedWork W2787259960 @default.
• W2159569892 hasRelatedWork W4306316640 @default.
• W2159569892 hasVolume "182" @default.

• next