Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159630078> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2159630078 endingPage "498" @default.
- W2159630078 startingPage "493" @default.
- W2159630078 abstract "Cryptosporidiosis is an important zoonosis caused by the Cryptosporidium species. To develop a PCR diagnostic kit for molecular detection and differential diagnosis of Cryptosporidium spp., a portion of ITS-1 sequence of Cryptosporidium. andersoni was chosen as the target DNA for designing the species-specific primers (ZR QF/ZR). The kit components were determined after the PCR amplification conditions were serially optimized. A series of tests were conducted in the specificity, sensitivity, stability, reproducibility, and stored period of the kit, respectively. The results showed that only C. andersoni were amplified specific band of about 500 bp, while Cryptosporidium. parvum, Cryptosporidium. baileyi, Eimeria sp of dairy cow, Toxoplasma gondii, Eimeria sp of pig, Ascaris suum, Cyclospora sp, and E. coli could not be amplified. 254 oocysts of C. andersoni was the lowest number that could be detected using the kit. The kit worked well after being stored at room temperature, 4 and −20°C for nine months. Fecal specimens, which were collected from a total of 243 calves on four different dairy farms in Guangdong Province, China, and one dairy farm in Henan Province, China, were examined using the kit; the positive rate of the kit was 2–13% higher than that of the routine methods. The results indicated that the kit can detect fecal samples faster, more sensitively, and conveniently, and can provide a useful tool for the identification and differentiation of C. andersoni from the other Cryptosporidium species; it also has implications for further studies on molecular epidemiology and differential diagnostics of cryptosporidiosis in animals." @default.
- W2159630078 created "2016-06-24" @default.
- W2159630078 creator A5031741004 @default.
- W2159630078 creator A5042210496 @default.
- W2159630078 creator A5061739686 @default.
- W2159630078 creator A5085428650 @default.
- W2159630078 date "2007-04-01" @default.
- W2159630078 modified "2023-09-26" @default.
- W2159630078 title "Development of a PCR Diagnostic Kit for Cryptosporidium andersoni in Dairy Cow" @default.
- W2159630078 cites W1540180694 @default.
- W2159630078 cites W2000666928 @default.
- W2159630078 cites W2000997095 @default.
- W2159630078 cites W2019402469 @default.
- W2159630078 cites W2030332655 @default.
- W2159630078 cites W2049276002 @default.
- W2159630078 cites W2067475113 @default.
- W2159630078 cites W2080060869 @default.
- W2159630078 cites W2093702487 @default.
- W2159630078 cites W2109576956 @default.
- W2159630078 cites W2122441393 @default.
- W2159630078 cites W2126983680 @default.
- W2159630078 cites W2142878327 @default.
- W2159630078 cites W2155485769 @default.
- W2159630078 cites W2162799953 @default.
- W2159630078 cites W2332810979 @default.
- W2159630078 cites W2999507306 @default.
- W2159630078 doi "https://doi.org/10.1016/s1671-2927(07)60074-1" @default.
- W2159630078 hasPublicationYear "2007" @default.
- W2159630078 type Work @default.
- W2159630078 sameAs 2159630078 @default.
- W2159630078 citedByCount "1" @default.
- W2159630078 countsByYear W21596300782015 @default.
- W2159630078 crossrefType "journal-article" @default.
- W2159630078 hasAuthorship W2159630078A5031741004 @default.
- W2159630078 hasAuthorship W2159630078A5042210496 @default.
- W2159630078 hasAuthorship W2159630078A5061739686 @default.
- W2159630078 hasAuthorship W2159630078A5085428650 @default.
- W2159630078 hasConcept C104317684 @default.
- W2159630078 hasConcept C140793950 @default.
- W2159630078 hasConcept C165901193 @default.
- W2159630078 hasConcept C2776977481 @default.
- W2159630078 hasConcept C2777795018 @default.
- W2159630078 hasConcept C2777976947 @default.
- W2159630078 hasConcept C2778877831 @default.
- W2159630078 hasConcept C2779845534 @default.
- W2159630078 hasConcept C42972112 @default.
- W2159630078 hasConcept C49105822 @default.
- W2159630078 hasConcept C54355233 @default.
- W2159630078 hasConcept C61716771 @default.
- W2159630078 hasConcept C71924100 @default.
- W2159630078 hasConcept C86803240 @default.
- W2159630078 hasConcept C89423630 @default.
- W2159630078 hasConcept C90856448 @default.
- W2159630078 hasConceptScore W2159630078C104317684 @default.
- W2159630078 hasConceptScore W2159630078C140793950 @default.
- W2159630078 hasConceptScore W2159630078C165901193 @default.
- W2159630078 hasConceptScore W2159630078C2776977481 @default.
- W2159630078 hasConceptScore W2159630078C2777795018 @default.
- W2159630078 hasConceptScore W2159630078C2777976947 @default.
- W2159630078 hasConceptScore W2159630078C2778877831 @default.
- W2159630078 hasConceptScore W2159630078C2779845534 @default.
- W2159630078 hasConceptScore W2159630078C42972112 @default.
- W2159630078 hasConceptScore W2159630078C49105822 @default.
- W2159630078 hasConceptScore W2159630078C54355233 @default.
- W2159630078 hasConceptScore W2159630078C61716771 @default.
- W2159630078 hasConceptScore W2159630078C71924100 @default.
- W2159630078 hasConceptScore W2159630078C86803240 @default.
- W2159630078 hasConceptScore W2159630078C89423630 @default.
- W2159630078 hasConceptScore W2159630078C90856448 @default.
- W2159630078 hasIssue "4" @default.
- W2159630078 hasLocation W21596300781 @default.
- W2159630078 hasOpenAccess W2159630078 @default.
- W2159630078 hasPrimaryLocation W21596300781 @default.
- W2159630078 hasRelatedWork W2075847904 @default.
- W2159630078 hasRelatedWork W2084257432 @default.
- W2159630078 hasRelatedWork W2088917112 @default.
- W2159630078 hasRelatedWork W2094748879 @default.
- W2159630078 hasRelatedWork W2111538377 @default.
- W2159630078 hasRelatedWork W2154310774 @default.
- W2159630078 hasRelatedWork W2357802184 @default.
- W2159630078 hasRelatedWork W3158056715 @default.
- W2159630078 hasRelatedWork W337470490 @default.
- W2159630078 hasRelatedWork W2416780450 @default.
- W2159630078 hasVolume "6" @default.
- W2159630078 isParatext "false" @default.
- W2159630078 isRetracted "false" @default.
- W2159630078 magId "2159630078" @default.
- W2159630078 workType "article" @default.