Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159800348> ?p ?o ?g. }
- W2159800348 endingPage "1329" @default.
- W2159800348 startingPage "1323" @default.
- W2159800348 abstract "We have characterized the determinants of methotrexate (MTX) responsiveness in eight patient-derived cell lines of small-cell lung cancer (SCLC). Clonogenic survival was correlated with factors known to affect sensitivity to drug. NCI-H209 and NCI-H128 were most drug sensitive, with drug concentrations required to inhibit clonogenic survival by 50% with less than 0.1 microM MTX. Six cell lines (NCI-H187, NCI-H345, NCI-H60, NCI-H524, NCI-H146, and NCI-N417D) were relatively drug resistant. In all cell lines studied, higher molecular weight MTX-polyglutamates (MTX-PGs) with 3-5 glutamyl moieties (MTX-Glu3 through MTX-Glu5) were selectively retained. Relative resistance to low (1.0 microM) drug concentrations appeared to be largely due to decreased intracellular metabolism of MTX. Five of the six resistant lines were able to synthesize polyglutamates at higher (10 microM) drug concentrations, although one resistant cell line (NCI-N417D) did not synthesize higher molecular weight MTX-PGs, even after exposure to 10 microM drug. Two cell lines with resistance to 10 microM MTX (NCI-H146 and NCI-H524) synthesized and retained higher molecular weight MTX-PGs in excess of binding capacity after exposure to 10 microM drug. However, the specific activity of thymidylate synthase in these cell lines was low. MTX sensitivity in patient-derived cell lines of SCLC requires the ability of cells to accumulate and retain intracellular drug in the form of polyglutamate metabolites in excess of dihydrofolate reductase, as well as a high basal level of consumption of reduced folates in the synthesis of thymidylate." @default.
- W2159800348 created "2016-06-24" @default.
- W2159800348 creator A5010199108 @default.
- W2159800348 creator A5014060948 @default.
- W2159800348 creator A5016346099 @default.
- W2159800348 creator A5067138875 @default.
- W2159800348 creator A5072276939 @default.
- W2159800348 creator A5080163482 @default.
- W2159800348 creator A5089927948 @default.
- W2159800348 date "1985-10-01" @default.
- W2159800348 modified "2023-10-18" @default.
- W2159800348 title "Determinants of the sensitivity of human small-cell lung cancer cell lines to methotrexate." @default.
- W2159800348 cites W1489298252 @default.
- W2159800348 cites W1494464160 @default.
- W2159800348 cites W1501146921 @default.
- W2159800348 cites W1507212424 @default.
- W2159800348 cites W1509635893 @default.
- W2159800348 cites W1539851476 @default.
- W2159800348 cites W1540832344 @default.
- W2159800348 cites W1540897124 @default.
- W2159800348 cites W1543817477 @default.
- W2159800348 cites W1559420331 @default.
- W2159800348 cites W1560690111 @default.
- W2159800348 cites W1572081719 @default.
- W2159800348 cites W1605180802 @default.
- W2159800348 cites W1775749144 @default.
- W2159800348 cites W1921208916 @default.
- W2159800348 cites W195608166 @default.
- W2159800348 cites W1958376989 @default.
- W2159800348 cites W1978512571 @default.
- W2159800348 cites W1983449164 @default.
- W2159800348 cites W1992673437 @default.
- W2159800348 cites W2003098361 @default.
- W2159800348 cites W2008582329 @default.
- W2159800348 cites W2036544276 @default.
- W2159800348 cites W2042378550 @default.
- W2159800348 cites W2051760741 @default.
- W2159800348 cites W2073018439 @default.
- W2159800348 cites W2073107168 @default.
- W2159800348 cites W2091018813 @default.
- W2159800348 cites W2097495404 @default.
- W2159800348 cites W2106625719 @default.
- W2159800348 cites W2107241699 @default.
- W2159800348 cites W2178402621 @default.
- W2159800348 cites W2241733037 @default.
- W2159800348 cites W2256754836 @default.
- W2159800348 cites W2320302422 @default.
- W2159800348 cites W2339354081 @default.
- W2159800348 cites W2346994988 @default.
- W2159800348 cites W2407270222 @default.
- W2159800348 cites W2413211457 @default.
- W2159800348 cites W2418104355 @default.
- W2159800348 cites W2418513986 @default.
- W2159800348 cites W2441915155 @default.
- W2159800348 cites W4253186968 @default.
- W2159800348 doi "https://doi.org/10.1172/jci112106" @default.
- W2159800348 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/424067" @default.
- W2159800348 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2414316" @default.
- W2159800348 hasPublicationYear "1985" @default.
- W2159800348 type Work @default.
- W2159800348 sameAs 2159800348 @default.
- W2159800348 citedByCount "42" @default.
- W2159800348 countsByYear W21598003482014 @default.
- W2159800348 countsByYear W21598003482015 @default.
- W2159800348 countsByYear W21598003482017 @default.
- W2159800348 countsByYear W21598003482022 @default.
- W2159800348 crossrefType "journal-article" @default.
- W2159800348 hasAuthorship W2159800348A5010199108 @default.
- W2159800348 hasAuthorship W2159800348A5014060948 @default.
- W2159800348 hasAuthorship W2159800348A5016346099 @default.
- W2159800348 hasAuthorship W2159800348A5067138875 @default.
- W2159800348 hasAuthorship W2159800348A5072276939 @default.
- W2159800348 hasAuthorship W2159800348A5080163482 @default.
- W2159800348 hasAuthorship W2159800348A5089927948 @default.
- W2159800348 hasBestOaLocation W21598003481 @default.
- W2159800348 hasConcept C117262875 @default.
- W2159800348 hasConcept C121608353 @default.
- W2159800348 hasConcept C123321153 @default.
- W2159800348 hasConcept C126322002 @default.
- W2159800348 hasConcept C1491633281 @default.
- W2159800348 hasConcept C185592680 @default.
- W2159800348 hasConcept C203014093 @default.
- W2159800348 hasConcept C2778397455 @default.
- W2159800348 hasConcept C2780035454 @default.
- W2159800348 hasConcept C2780456651 @default.
- W2159800348 hasConcept C2781059491 @default.
- W2159800348 hasConcept C2781320022 @default.
- W2159800348 hasConcept C2781382166 @default.
- W2159800348 hasConcept C502942594 @default.
- W2159800348 hasConcept C54355233 @default.
- W2159800348 hasConcept C55493867 @default.
- W2159800348 hasConcept C71924100 @default.
- W2159800348 hasConcept C79879829 @default.
- W2159800348 hasConcept C81885089 @default.
- W2159800348 hasConcept C86803240 @default.
- W2159800348 hasConcept C98274493 @default.
- W2159800348 hasConceptScore W2159800348C117262875 @default.
- W2159800348 hasConceptScore W2159800348C121608353 @default.