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- W2159800474 abstract "<h3>Abstract</h3> The formation of differential cell lineages in the mammalian blastocyst from the totipotent zygote is crucial for implantation and the success of the whole pregnancy. The first lineage segregation generates the polarised trophectoderm (TE) tissue, which forms the placenta, and the apolar inner cell mass (ICM), which mainly gives rise to all foetal tissues and also the yolk sac<sup>1–3</sup>. The mechanism underlying this cell fate segregation has been extensively studied in the mouse embryo<sup>4,5</sup>. However, when and how it takes place in the human embryo remains unclear. Here, using time-lapse imaging and 325 surplus human embryos, we provide a detailed characterisation of morphological events and transcription factor expression and localisation to understand how they lead to the first lineage segregation in human embryogenesis. We show that the first lineage segregation of the human embryo is triggered by cell polarisation that occurs at the 8-cell stage in two sequential steps. In the first step, F-actin becomes apically polarised concomitantly with embryo compaction. In the second step, the Par complex becomes polarised to form the apical cellular domain. Mechanistically, we show that activation of Phospholipase C (PLC) triggers actin polarisation and is therefore essential for apical domain formation, as is the case in mouse embryos<sup>6</sup>. Finally, we show that, in contrast to the mouse embryo, the key extra-embryonic determinant GATA3<sup>7,8</sup> is expressed not only in extra-embryonic lineage precursors upon blastocyst formation. However, the cell polarity machinery enhances the expression and nuclear accumulation of GATA3. In summary, our results demonstrate for the first time that cell polarisation reinforces the first lineage segregation in the human embryo." @default.
- W2159800474 created "2016-06-24" @default.
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- W2159800474 date "1979-08-01" @default.
- W2159800474 modified "2023-09-29" @default.
- W2159800474 title "Winter vomiting disease caused by calicivirus." @default.
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- W2159800474 doi "https://doi.org/10.1136/jcp.32.8.786" @default.
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