Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159801277> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2159801277 endingPage "182" @default.
- W2159801277 startingPage "174" @default.
- W2159801277 abstract "Most cases of Escherichia coli K1 meningitis arise as a result of haematogenous spread, however there is a limited understanding of the mechanisms by which circulating E. coli K1 cross the blood-brain barrier. We have previously shown that environmental growth conditions both positively and negatively influence the capabilities of E. coli K1 to invade brain microvascular endothelial cells (BMEC), for example growth in media supplemented with 50% newborn bovine serum (NBS) increased BMEC invasion, whereas growth in media supplemented with 0.2 M NaCl repressed invasion in vitro and in vivo. In this study, differential fluorescence induction (DFI) was used to identify E. coli K1 genes involved in this differentially expressed invasion phenotype. E. coli K1 promoter libraries were constructed and screened for gfp expression in a manner analogous to the above growth conditions. Twenty-four clones were isolated that showed fluorescence induction when grown under the invasion-enhancing condition (i.e. NBS). Four of these clones also demonstrated repression or no induction of fluorescence when grown under the invasion-repressing condition (i.e. 0.2 M NaCl). One such clone, containing a ygdP promoter and an open reading frame (ORF), showed significant homology to Bartonella bacilliformis IalA (invasion associated locus). Among the other NBS-inducing loci, finPtraJ was identified as well as several clones with no homology to other known genes. When ygdP, finPtraJ and several of the unique loci were disrupted in E. coli K1, there was a significant decrease in human BMEC (HBMEC) invasion. RNA transcript analysis determined that these newly identified invasion loci were differentially regulated at the transcriptional level. This is the first demonstration of using DFI to identify E. coli K1 genes contributing to HBMEC invasion." @default.
- W2159801277 created "2016-06-24" @default.
- W2159801277 creator A5010667884 @default.
- W2159801277 creator A5016165244 @default.
- W2159801277 creator A5064480406 @default.
- W2159801277 date "2000-04-01" @default.
- W2159801277 modified "2023-10-11" @default.
- W2159801277 title "Identification of Escherichia coli K1 genes contributing to human brain microvascular endothelial cell invasion by differential fluorescence induction" @default.
- W2159801277 cites W1885560386 @default.
- W2159801277 cites W1892528326 @default.
- W2159801277 cites W1956745113 @default.
- W2159801277 cites W1964324020 @default.
- W2159801277 cites W1979649993 @default.
- W2159801277 cites W2027229485 @default.
- W2159801277 cites W2078508999 @default.
- W2159801277 cites W2099686694 @default.
- W2159801277 cites W2105143050 @default.
- W2159801277 cites W2111092197 @default.
- W2159801277 cites W2111980950 @default.
- W2159801277 cites W2128380342 @default.
- W2159801277 cites W2133552199 @default.
- W2159801277 cites W2134153749 @default.
- W2159801277 cites W2144373247 @default.
- W2159801277 cites W2152362181 @default.
- W2159801277 cites W2171498110 @default.
- W2159801277 cites W2413264397 @default.
- W2159801277 cites W4233808990 @default.
- W2159801277 cites W4239199313 @default.
- W2159801277 doi "https://doi.org/10.1046/j.1365-2958.2000.01840.x" @default.
- W2159801277 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10760174" @default.
- W2159801277 hasPublicationYear "2000" @default.
- W2159801277 type Work @default.
- W2159801277 sameAs 2159801277 @default.
- W2159801277 citedByCount "93" @default.
- W2159801277 countsByYear W21598012772012 @default.
- W2159801277 countsByYear W21598012772013 @default.
- W2159801277 countsByYear W21598012772014 @default.
- W2159801277 countsByYear W21598012772015 @default.
- W2159801277 countsByYear W21598012772016 @default.
- W2159801277 countsByYear W21598012772017 @default.
- W2159801277 countsByYear W21598012772018 @default.
- W2159801277 countsByYear W21598012772019 @default.
- W2159801277 countsByYear W21598012772020 @default.
- W2159801277 countsByYear W21598012772022 @default.
- W2159801277 countsByYear W21598012772023 @default.
- W2159801277 crossrefType "journal-article" @default.
- W2159801277 hasAuthorship W2159801277A5010667884 @default.
- W2159801277 hasAuthorship W2159801277A5016165244 @default.
- W2159801277 hasAuthorship W2159801277A5064480406 @default.
- W2159801277 hasBestOaLocation W21598012771 @default.
- W2159801277 hasConcept C104317684 @default.
- W2159801277 hasConcept C153911025 @default.
- W2159801277 hasConcept C167625842 @default.
- W2159801277 hasConcept C47289529 @default.
- W2159801277 hasConcept C54355233 @default.
- W2159801277 hasConcept C547475151 @default.
- W2159801277 hasConcept C81089528 @default.
- W2159801277 hasConcept C86803240 @default.
- W2159801277 hasConcept C89423630 @default.
- W2159801277 hasConceptScore W2159801277C104317684 @default.
- W2159801277 hasConceptScore W2159801277C153911025 @default.
- W2159801277 hasConceptScore W2159801277C167625842 @default.
- W2159801277 hasConceptScore W2159801277C47289529 @default.
- W2159801277 hasConceptScore W2159801277C54355233 @default.
- W2159801277 hasConceptScore W2159801277C547475151 @default.
- W2159801277 hasConceptScore W2159801277C81089528 @default.
- W2159801277 hasConceptScore W2159801277C86803240 @default.
- W2159801277 hasConceptScore W2159801277C89423630 @default.
- W2159801277 hasIssue "1" @default.
- W2159801277 hasLocation W21598012771 @default.
- W2159801277 hasLocation W21598012772 @default.
- W2159801277 hasOpenAccess W2159801277 @default.
- W2159801277 hasPrimaryLocation W21598012771 @default.
- W2159801277 hasRelatedWork W1948731625 @default.
- W2159801277 hasRelatedWork W1987905608 @default.
- W2159801277 hasRelatedWork W2003630654 @default.
- W2159801277 hasRelatedWork W2027674732 @default.
- W2159801277 hasRelatedWork W2045313050 @default.
- W2159801277 hasRelatedWork W2076127294 @default.
- W2159801277 hasRelatedWork W2216804349 @default.
- W2159801277 hasRelatedWork W2361865054 @default.
- W2159801277 hasRelatedWork W2498907773 @default.
- W2159801277 hasRelatedWork W1800235398 @default.
- W2159801277 hasVolume "36" @default.
- W2159801277 isParatext "false" @default.
- W2159801277 isRetracted "false" @default.
- W2159801277 magId "2159801277" @default.
- W2159801277 workType "article" @default.