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• W2159849893 abstract "To provide a rapid and sensitive method for detecting NoV GI and NoV GII in water and to evaluate the use of the murine norovirus (MNV-1) as a process control.The method is based on viral concentration by filtration on electropositive filters and direct lysis of adsorbed viruses from filters before RNA extraction and RT-qPCR amplification. An one-step multiplex RT-qPCR assay was developed for the simultaneous detection of NoV GI, NoV GII and MNV-1. Then, water samples were artificially contaminated to determine mean virus recoveries and method sensitivity. The method showed a higher sensitivity for detecting NoV GII (10(3) genome copies per 0·5 l) than for NoV GI (10(4) genome copies per 0·5 l) in the presence of MNV-1 regardless of the type of water. The data also showed that MNV-1 is a robust option as process control.The method described provides a valuable tool for the monitoring of potential public health risks associated with NoV contamination in drinkable water.Given the increasing evidence for NoV involvement in food outbreaks, the one-step multiplex RT-qPCR assay we used in this study would be a very useful tool to investigate NoV contamination in other food products." @default.
• W2159849893 created "2016-06-24" @default.
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• W2159849893 date "2013-10-16" @default.
• W2159849893 modified "2023-10-02" @default.
• W2159849893 title "Multiplex real-time RT-qPCR for the detection of Norovirus in bottled and tap water using murine norovirus as a process control" @default.
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• W2159849893 doi "https://doi.org/10.1111/jam.12345" @default.
• W2159849893 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24102819" @default.
• W2159849893 hasPublicationYear "2013" @default.
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