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- W2159854313 abstract "Insect genome projects and DNA sequence databases are providing unprecedented amounts of information about variation at specific nucleotides in protein- and RNA-coding genes. Single nucleotide polymorphisms (SNPs) are abundant in all insect species so far examined and are proving useful in population genetics, linkage mapping and marker-assisted selection. A number of studies has already identified SNPs associated with insecticide resistance, especially mutations conferring reduced target site sensitivity. Unfortunately, most modern, high-throughput, automated SNP detection technologies are expensive or require the use of expensive equipment and are therefore not accessible to laboratories on a limited budget or to our colleagues in developing countries. In this review, we provide a chronological and comprehensive list of all SNP methods. We emphasize and explain those techniques in which genotypes can be identified by eye or that only require agarose gel electrophoresis. We provide examples where these techniques have or are currently being applied to insects." @default.
- W2159854313 created "2016-06-24" @default.
- W2159854313 creator A5001738436 @default.
- W2159854313 creator A5019599810 @default.
- W2159854313 date "2007-08-01" @default.
- W2159854313 modified "2023-10-16" @default.
- W2159854313 title "Affordable assays for genotyping single nucleotide polymorphisms in insects" @default.
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- W2159854313 doi "https://doi.org/10.1111/j.1365-2583.2007.00736.x" @default.
- W2159854313 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17488301" @default.
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