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• W2159889514 abstract "Increasing evidence demonstrates that miRNAs are involved in the dysregulation of tumor initiating cells (TICs) in various tumors. Due to a lack of definitive markers, cell sorting is not an ideal separation method for lung adenocarcinoma initiating cells. In this study, we combined paclitaxel with serum-free medium cultivation (inverse-induction) to enrich TICs from A549 cells, marked by CD133/CD326, defined features of stemness. We next investigated aberrant microRNAs in this subpopulation compared to normal cells with miRNA microarray and found that 50 miRNAs exhibited a greater than 2-fold change in expression. As further validation, 10 miRNAs were chosen to perform quantitative RT-PCR on the A549 cell line and primary samples. The results suggest that aberrant expression of miRNAs such as miR-29ab, miR-183, miR-17-5p and miR-127-3P may play an important role in regulating the bio-behavior of TICs." @default.
• W2159889514 created "2016-06-24" @default.
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• W2159889514 date "2012-02-15" @default.
• W2159889514 modified "2023-10-08" @default.
• W2159889514 title "Aberrant microRNAs expression in CD133+/CD326+ human lung adenocarcinoma initiating cells from A549" @default.
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• W2159889514 doi "https://doi.org/10.1007/s10059-012-2252-y" @default.
• W2159889514 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3887701" @default.
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