FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2159940897> ?p ?o ?g. }

• W2159940897 endingPage "125" @default.
• W2159940897 startingPage "113" @default.
• W2159940897 abstract "The aim of the present study was to investigate the effects of activin-A and follistatin on in vitro primordial and primary follicle development in goats. To study primordial follicle development (experiment 1), pieces of ovarian cortex were cultured in vitro for 5 days in minimal essential medium (MEM) supplemented with activin-A (0, 10 or 100 ng/ml), follistatin (0, 10 or 100 ng/ml) or combinations of the two. After culture, the numbers of primordial follicles and more advanced follicle stages were calculated and compared with those in non-cultured tissue. Protein and mRNA expression of activin-A, follistatin, Kit ligand (KL), growth differentiation factor-9 (GDF-9) and bone morphogenetic protein-15 (BMP-15) in non-cultured and cultured follicles were studied by immunohistochemistry and PCR. To evaluate primary follicle growth (experiment 2), freshly isolated follicles were cultured for 6 days in MEM plus 100 ng/ml activin-A, 100 ng/ml follistatin or 100 ng/ml activin-A plus 200 ng/ml follistatin. Morphology, follicle and oocyte diameters in cultured tissue and isolated follicles before and after culture were assessed. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) reactions were performed to study DNA fragmentation in follicles. In experiment 1, it was found that goat primordial follicles were activated to develop into more advanced stages, i.e. intermediate and primary follicles, during in vitro culture, but neither activin-A nor follistatin affected the number of primordial follicles that entered the growth phase. Activin-A treatment enhanced the number of morphologically normal follicles and stimulated their growth during cortical tissue culture. The effects were, however, not counteracted by follistatin. The follicles in cultured goat tissue maintained their expression of proteins and mRNA for activin-A, follistatin, KL, GDF-9 and BMP-15. Fewer than 30% of the atretic follicles in cultured cortical tissue had TUNEL-positive (oocyte or granulosa) cells. Activin-A did not affect the occurrence of TUNEL-positive cells in follicles within cortical tissue. In experiment 2, addition of activin-A to cultured isolated primary follicles significantly stimulated their growth, the effect being counteracted by follistatin. Absence of such a neutralizing effect of follistatin in the cultures with ovarian cortical tissue can be due to lower dose of follistatin used and incomplete blockage of activin in these experiments. In contrast to cortical enclosed atretic follicles, all atretic follicles that had arisen in cultures with isolated primary follicles had TUNEL-positive cells, which points to differences between isolated and ovarian tissue-enclosed follicles with regard to the followed pathways leading to their degeneration. In summary, this in vitro study has demonstrated that cultured goat primordial follicles are activated to grow and develop into intermediate and primary follicles. During in vitro culture, the follicles maintain their ability to express activin-A, follistatin, KL, GDF-9 and BMP-15. The in vitro growth and survival of activated follicles enclosed in cortical tissue and the in vitro growth of isolated primary follicles are stimulated by activin-A." @default.
• W2159940897 created "2016-06-24" @default.
• W2159940897 creator A5004601434 @default.
• W2159940897 creator A5024125034 @default.
• W2159940897 creator A5035996044 @default.
• W2159940897 creator A5036049868 @default.
• W2159940897 creator A5043333015 @default.
• W2159940897 creator A5052710548 @default.
• W2159940897 creator A5060605435 @default.
• W2159940897 date "2006-04-01" @default.
• W2159940897 modified "2023-10-16" @default.
• W2159940897 title "The activin-follistatin system and in vitro early follicle development in goats" @default.
• W2159940897 cites W1501791679 @default.
• W2159940897 cites W1735059796 @default.
• W2159940897 cites W1923939500 @default.
• W2159940897 cites W1963625504 @default.
• W2159940897 cites W1965369413 @default.
• W2159940897 cites W1968446974 @default.
• W2159940897 cites W1974757434 @default.
• W2159940897 cites W1982616308 @default.
• W2159940897 cites W1992201536 @default.
• W2159940897 cites W1993932453 @default.
• W2159940897 cites W1999100254 @default.
• W2159940897 cites W2016002306 @default.
• W2159940897 cites W2018831893 @default.
• W2159940897 cites W2018853148 @default.
• W2159940897 cites W2021534970 @default.
• W2159940897 cites W2036246831 @default.
• W2159940897 cites W2040525542 @default.
• W2159940897 cites W2042292319 @default.
• W2159940897 cites W2043215244 @default.
• W2159940897 cites W2050874941 @default.
• W2159940897 cites W2055984060 @default.
• W2159940897 cites W2061758394 @default.
• W2159940897 cites W2062024691 @default.
• W2159940897 cites W2079355671 @default.
• W2159940897 cites W2080926290 @default.
• W2159940897 cites W2086949490 @default.
• W2159940897 cites W2110932329 @default.
• W2159940897 cites W2112542261 @default.
• W2159940897 cites W2113746705 @default.
• W2159940897 cites W2121853087 @default.
• W2159940897 cites W2127910408 @default.
• W2159940897 cites W2131593655 @default.
• W2159940897 cites W2135104064 @default.
• W2159940897 cites W2140491489 @default.
• W2159940897 cites W2141777619 @default.
• W2159940897 cites W2142250901 @default.
• W2159940897 cites W2142478448 @default.
• W2159940897 cites W2143932116 @default.
• W2159940897 cites W2148839199 @default.
• W2159940897 cites W2150845522 @default.
• W2159940897 cites W2154957337 @default.
• W2159940897 cites W2155637594 @default.
• W2159940897 cites W2157658339 @default.
• W2159940897 cites W2161105368 @default.
• W2159940897 doi "https://doi.org/10.1677/joe.1.06487" @default.
• W2159940897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16614386" @default.
• W2159940897 hasPublicationYear "2006" @default.
• W2159940897 type Work @default.
• W2159940897 sameAs 2159940897 @default.
• W2159940897 citedByCount "54" @default.
• W2159940897 countsByYear W21599408972012 @default.
• W2159940897 countsByYear W21599408972013 @default.
• W2159940897 countsByYear W21599408972014 @default.
• W2159940897 countsByYear W21599408972015 @default.
• W2159940897 countsByYear W21599408972016 @default.
• W2159940897 countsByYear W21599408972017 @default.
• W2159940897 countsByYear W21599408972018 @default.
• W2159940897 countsByYear W21599408972019 @default.
• W2159940897 countsByYear W21599408972020 @default.
• W2159940897 countsByYear W21599408972021 @default.
• W2159940897 countsByYear W21599408972022 @default.
• W2159940897 countsByYear W21599408972023 @default.
• W2159940897 crossrefType "journal-article" @default.
• W2159940897 hasAuthorship W2159940897A5004601434 @default.
• W2159940897 hasAuthorship W2159940897A5024125034 @default.
• W2159940897 hasAuthorship W2159940897A5035996044 @default.
• W2159940897 hasAuthorship W2159940897A5036049868 @default.
• W2159940897 hasAuthorship W2159940897A5043333015 @default.
• W2159940897 hasAuthorship W2159940897A5052710548 @default.
• W2159940897 hasAuthorship W2159940897A5060605435 @default.
• W2159940897 hasBestOaLocation W21599408971 @default.
• W2159940897 hasConcept C102570824 @default.
• W2159940897 hasConcept C126322002 @default.
• W2159940897 hasConcept C134018914 @default.
• W2159940897 hasConcept C16685009 @default.
• W2159940897 hasConcept C196843134 @default.
• W2159940897 hasConcept C202751555 @default.
• W2159940897 hasConcept C2777216303 @default.
• W2159940897 hasConcept C2778324911 @default.
• W2159940897 hasConcept C2778831791 @default.
• W2159940897 hasConcept C2780536345 @default.
• W2159940897 hasConcept C5512455 @default.
• W2159940897 hasConcept C55493867 @default.
• W2159940897 hasConcept C71924100 @default.
• W2159940897 hasConcept C86803240 @default.
• W2159940897 hasConcept C87073359 @default.

• next