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• W2159958497 abstract "An interaction study was performed with mycorrhizal plants of the grapevine rootstock Richter 110 (Vitis berlandieri Planch × Vitis rupestris Scheele) and the root pathogenic fungus Armillaria mellea (Vahl:Fr.) P. Kumm using an autotrophic in vitro culture system. Micropropagated plantlets were transferred to Petri plates with MSR medium lacking sugar and vitamins. Inocula of Glomus intraradices (BEG 72) and of Armillaria mellea obtained from a root organ culture and from a mycelium colony grown in malt agar respectively, were added to the plates according to each treatment: non-inoculated, inoculated with G. intraradices, inoculated with A. mellea, and dual-inoculated plants. There were ten replicates per treatment. Fourteen weeks later, the pathogen's mycelium occupied most of the surface/volume of the plate and had produced rhizomorphs. In dual inoculated plates, A. mellea's growth was not affected by the presence of G. intraradices, but the latter produced a lower number of spores and its extraradical phase showed granulation, vacuolation and tip swelling. The pathogen induced necrosis and growth decrease in the root. Glomus intraradices alleviated these symptoms and there were no differences in root biomass between non-inoculated plants and plants inoculated with both fungi. There were no symptoms of the disease in shoots and G. intraradices stimulated shoot growth both, although mycorrhizal colonization was lower when A. mellea was present. No direct antagonism or antibiosis against the pathogen was observed, thus the protective effect exerted by the symbiotic fungus in grapevines must be indirect, mediated through the host plant physiology." @default.
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• W2159958497 date "2010-07-01" @default.
• W2159958497 modified "2023-10-18" @default.
• W2159958497 title "In vitro interaction studies between Glomus intraradices and Armillaria mellea in vines" @default.
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• W2159958497 doi "https://doi.org/10.5424/sjar/201008s1-1223" @default.
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