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- W2159963105 abstract "Summary - High performance capillary electrophoresis (CE) is a family of related microanalytical preparative techniques for the rapid separation of proteins, peptides oligonucleotides, and oligosaccharides. This article describes the use of CE as a tool to differentiate between representative populations of pathotypes of the potato cyst nematode species Globodera rostochiensis and G. pallida. Reproducible polypeptide profiles obtained by sodium dodecyl sulphate (SDS)-capillary gel electrophoresis displayed sorne peptides characteristic of G. rostochiensis and others characteristic of G. pallida. Polypeptide profues of each of the pathotypes of G. roswchiensis Ro l, R02, R03, R04, RoS, and G. pallida Pa l, Pa2IPa3 were obtained. Profiles of Australian isolates from Victoria were of the Ro 1 type and this pathotype classification was also determined by standard differential cultivar tests. The CE technique outlined is a reliable, reproducible technique which could aid with the differentiation of these pathotypes with minimum sample preparation. «~ Elsevier - ORSTOM" @default.
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- W2159963105 title "Discrimination of European and Australian Globodera rostochiensis and G. pallida pathotypes by high performance capillary electrophoresis." @default.
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