FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160040357> ?p ?o ?g. }

• W2160040357 endingPage "973" @default.
• W2160040357 startingPage "963" @default.
• W2160040357 abstract "Hydrophilic poly(ethylene glycol) diacrylate (PEGDA) hydrogel surfaces resist protein adsorption and are generally thought to be unsuitable for anchorage-dependent cells to adhere. Intriguingly, our previous findings revealed that PEGDA superporous hydrogel scaffolds (SPHs) allow anchorage of bone marrow derived human mesenchymal stem cells (hMSCs) and support their long-term survival. Therefore, we hypothesized that the physicochemical characteristics of the scaffold impart properties that could foster cellular responses. We examined if hMSCs alter their microenvironment to allow cell attachment by synthesizing their own extracellular matrix (ECM) proteins. Immunofluorescence staining revealed extensive expression of collagen type I, collagen type IV, laminin, and fibronectin within hMSC-seeded SPHs by the end of the third week. Whether cultured in serum-free or serum-supplemented medium, hMSC ECM protein gene expression patterns exhibited no substantial changes. The presence of serum proteins is required for initial anchorage of hMSCs within the SPHs but not for the hMSC survival after 24 h. In contrast to 2D expansion on tissue culture plastic (TCP), hMSCs cultured within SPHs proliferate similarly in the presence or absence of serum. To test whether hMSCs retain their undifferentiated state within the SPHs, cell-seeded constructs were cultured for 3 weeks in stem cell maintenance medium and the expression of hMSC-specific cell surface markers were evaluated by flow cytometry. CD105, CD90, CD73, and CD44 were present to a similar extent in the SPH and in 2D monolayer culture. We further demonstrated multilineage potential of hMSCs grown in the PEGDA SPHs, whereby differentiation into osteoblasts, chondrocytes, and adipocytes could be induced. The present study demonstrates the potential of hMSCs to alter the blank PEGDA environment to a milieu conducive to cell growth and multilineage differentiation by secreting adhesive ECM proteins within the porous network of the SPH scaffolds." @default.
• W2160040357 created "2016-06-24" @default.
• W2160040357 creator A5003957925 @default.
• W2160040357 creator A5016270298 @default.
• W2160040357 creator A5018808079 @default.
• W2160040357 creator A5038225154 @default.
• W2160040357 creator A5063744555 @default.
• W2160040357 creator A5083688157 @default.
• W2160040357 date "2012-03-22" @default.
• W2160040357 modified "2023-10-14" @default.
• W2160040357 title "Stem Cell-Derived Extracellular Matrix Enables Survival and Multilineage Differentiation within Superporous Hydrogels" @default.
• W2160040357 cites W1590052782 @default.
• W2160040357 cites W1590204800 @default.
• W2160040357 cites W1965953554 @default.
• W2160040357 cites W1978196155 @default.
• W2160040357 cites W1982923563 @default.
• W2160040357 cites W1983844422 @default.
• W2160040357 cites W1989380622 @default.
• W2160040357 cites W1990574061 @default.
• W2160040357 cites W1992831601 @default.
• W2160040357 cites W1996803044 @default.
• W2160040357 cites W2015856405 @default.
• W2160040357 cites W2022055461 @default.
• W2160040357 cites W2029962632 @default.
• W2160040357 cites W2031776886 @default.
• W2160040357 cites W2034004034 @default.
• W2160040357 cites W2036348189 @default.
• W2160040357 cites W2046568054 @default.
• W2160040357 cites W2051752053 @default.
• W2160040357 cites W2063697785 @default.
• W2160040357 cites W2075655507 @default.
• W2160040357 cites W2084293556 @default.
• W2160040357 cites W2090851652 @default.
• W2160040357 cites W2098756310 @default.
• W2160040357 cites W2102488850 @default.
• W2160040357 cites W2107649335 @default.
• W2160040357 cites W2114570899 @default.
• W2160040357 cites W2116500923 @default.
• W2160040357 cites W2120876378 @default.
• W2160040357 cites W2127109571 @default.
• W2160040357 cites W2131717731 @default.
• W2160040357 cites W2137022580 @default.
• W2160040357 cites W2142966824 @default.
• W2160040357 cites W2145139438 @default.
• W2160040357 cites W2159695799 @default.
• W2160040357 cites W2159999899 @default.
• W2160040357 doi "https://doi.org/10.1021/bm300332w" @default.
• W2160040357 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3322260" @default.
• W2160040357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22404228" @default.
• W2160040357 hasPublicationYear "2012" @default.
• W2160040357 type Work @default.
• W2160040357 sameAs 2160040357 @default.
• W2160040357 citedByCount "47" @default.
• W2160040357 countsByYear W21600403572012 @default.
• W2160040357 countsByYear W21600403572013 @default.
• W2160040357 countsByYear W21600403572014 @default.
• W2160040357 countsByYear W21600403572015 @default.
• W2160040357 countsByYear W21600403572016 @default.
• W2160040357 countsByYear W21600403572017 @default.
• W2160040357 countsByYear W21600403572018 @default.
• W2160040357 countsByYear W21600403572019 @default.
• W2160040357 countsByYear W21600403572020 @default.
• W2160040357 countsByYear W21600403572021 @default.
• W2160040357 countsByYear W21600403572022 @default.
• W2160040357 crossrefType "journal-article" @default.
• W2160040357 hasAuthorship W2160040357A5003957925 @default.
• W2160040357 hasAuthorship W2160040357A5016270298 @default.
• W2160040357 hasAuthorship W2160040357A5018808079 @default.
• W2160040357 hasAuthorship W2160040357A5038225154 @default.
• W2160040357 hasAuthorship W2160040357A5063744555 @default.
• W2160040357 hasAuthorship W2160040357A5083688157 @default.
• W2160040357 hasBestOaLocation W21600403572 @default.
• W2160040357 hasConcept C108586683 @default.
• W2160040357 hasConcept C116716535 @default.
• W2160040357 hasConcept C12554922 @default.
• W2160040357 hasConcept C136229726 @default.
• W2160040357 hasConcept C1491633281 @default.
• W2160040357 hasConcept C185592680 @default.
• W2160040357 hasConcept C188027245 @default.
• W2160040357 hasConcept C189165786 @default.
• W2160040357 hasConcept C198826908 @default.
• W2160040357 hasConcept C2779814568 @default.
• W2160040357 hasConcept C2780932548 @default.
• W2160040357 hasConcept C35496256 @default.
• W2160040357 hasConcept C49892992 @default.
• W2160040357 hasConcept C54355233 @default.
• W2160040357 hasConcept C55493867 @default.
• W2160040357 hasConcept C71924100 @default.
• W2160040357 hasConcept C81885089 @default.
• W2160040357 hasConcept C86492073 @default.
• W2160040357 hasConcept C86803240 @default.
• W2160040357 hasConcept C95444343 @default.
• W2160040357 hasConceptScore W2160040357C108586683 @default.
• W2160040357 hasConceptScore W2160040357C116716535 @default.
• W2160040357 hasConceptScore W2160040357C12554922 @default.
• W2160040357 hasConceptScore W2160040357C136229726 @default.
• W2160040357 hasConceptScore W2160040357C1491633281 @default.
• W2160040357 hasConceptScore W2160040357C185592680 @default.

• next