Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160052005> ?p ?o ?g. }
- W2160052005 endingPage "49643" @default.
- W2160052005 startingPage "49633" @default.
- W2160052005 abstract "The mechanism by which the neural cell adhesion molecule, N-CAM, mediates homophilic interactions between cells has been variously attributed to an isologous interaction of the third immunoglobulin (Ig) domain, to reciprocal binding of the two N-terminal Ig domains, or to reciprocal interactions of all five Ig domains. Here, we have used a panel of recombinant proteins in a bead binding assay, as well as transfected and primary cells, to clarify the molecular mechanism of N-CAM homophilic binding. The entire extracellular region of N-CAM mediated bead aggregation in a concentration- and temperature-dependent manner. Interactions of the N-terminal Ig domains, Ig1 and Ig2, were essential for bead binding, based on deletion and mutation experiments and on antibody inhibition studies. These findings were largely in accord with aggregation experiments using transfected L cells or primary chick brain cells. Additionally, maximal binding was dependent on the integrity of the intramolecular domain-domain interactions throughout the extracellular region. We propose that these interactions maintain the relative orientation of each domain in an optimal configuration for binding. Our results suggest that the role of Ig3 in homophilic binding is largely structural. Several Ig3-specific reagents failed to affect N-CAM binding on beads or on cells, while an inhibitory effect of an Ig3-specific monoclonal antibody is probably due to perturbations at the Ig2-Ig3 boundary. Thus, it appears that reciprocal interactions between Ig1 and Ig2 are necessary and sufficient for N-CAM homophilic binding, but that maximal binding requires the quaternary structure of the extracellular region defined by intramolecular domain-domain interactions." @default.
- W2160052005 created "2016-06-24" @default.
- W2160052005 creator A5022425980 @default.
- W2160052005 creator A5030901529 @default.
- W2160052005 creator A5035814482 @default.
- W2160052005 creator A5055826476 @default.
- W2160052005 creator A5068690410 @default.
- W2160052005 date "2004-11-01" @default.
- W2160052005 modified "2023-09-27" @default.
- W2160052005 title "Neural Cell Adhesion Molecule (N-CAM) Homophilic Binding Mediated by the Two N-terminal Ig Domains Is Influenced by Intramolecular Domain-Domain Interactions" @default.
- W2160052005 cites W1516340190 @default.
- W2160052005 cites W1599428638 @default.
- W2160052005 cites W1599958769 @default.
- W2160052005 cites W166833621 @default.
- W2160052005 cites W1969765223 @default.
- W2160052005 cites W1971829608 @default.
- W2160052005 cites W1973109495 @default.
- W2160052005 cites W1981141697 @default.
- W2160052005 cites W1984509666 @default.
- W2160052005 cites W2009259931 @default.
- W2160052005 cites W2023165966 @default.
- W2160052005 cites W2026638655 @default.
- W2160052005 cites W2035500342 @default.
- W2160052005 cites W2037466167 @default.
- W2160052005 cites W2045213966 @default.
- W2160052005 cites W2048076819 @default.
- W2160052005 cites W2051429491 @default.
- W2160052005 cites W2053247155 @default.
- W2160052005 cites W2057329300 @default.
- W2160052005 cites W2061176200 @default.
- W2160052005 cites W2063559096 @default.
- W2160052005 cites W2067495948 @default.
- W2160052005 cites W2067707319 @default.
- W2160052005 cites W2068495101 @default.
- W2160052005 cites W2069911462 @default.
- W2160052005 cites W2070141228 @default.
- W2160052005 cites W2075574902 @default.
- W2160052005 cites W2081029926 @default.
- W2160052005 cites W2082591764 @default.
- W2160052005 cites W2092017901 @default.
- W2160052005 cites W2092498617 @default.
- W2160052005 cites W2097983665 @default.
- W2160052005 cites W2123541453 @default.
- W2160052005 cites W2141699093 @default.
- W2160052005 cites W2155895966 @default.
- W2160052005 cites W2165074047 @default.
- W2160052005 cites W2165278371 @default.
- W2160052005 cites W2166701612 @default.
- W2160052005 cites W2177077947 @default.
- W2160052005 cites W81558796 @default.
- W2160052005 doi "https://doi.org/10.1074/jbc.m409159200" @default.
- W2160052005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15381695" @default.
- W2160052005 hasPublicationYear "2004" @default.
- W2160052005 type Work @default.
- W2160052005 sameAs 2160052005 @default.
- W2160052005 citedByCount "15" @default.
- W2160052005 countsByYear W21600520052012 @default.
- W2160052005 countsByYear W21600520052013 @default.
- W2160052005 countsByYear W21600520052021 @default.
- W2160052005 crossrefType "journal-article" @default.
- W2160052005 hasAuthorship W2160052005A5022425980 @default.
- W2160052005 hasAuthorship W2160052005A5030901529 @default.
- W2160052005 hasAuthorship W2160052005A5035814482 @default.
- W2160052005 hasAuthorship W2160052005A5055826476 @default.
- W2160052005 hasAuthorship W2160052005A5068690410 @default.
- W2160052005 hasBestOaLocation W21600520051 @default.
- W2160052005 hasConcept C104317684 @default.
- W2160052005 hasConcept C107824862 @default.
- W2160052005 hasConcept C12554922 @default.
- W2160052005 hasConcept C1491633281 @default.
- W2160052005 hasConcept C159654299 @default.
- W2160052005 hasConcept C185592680 @default.
- W2160052005 hasConcept C25166345 @default.
- W2160052005 hasConcept C28406088 @default.
- W2160052005 hasConcept C51639874 @default.
- W2160052005 hasConcept C54009773 @default.
- W2160052005 hasConcept C54355233 @default.
- W2160052005 hasConcept C55493867 @default.
- W2160052005 hasConcept C85789140 @default.
- W2160052005 hasConcept C86803240 @default.
- W2160052005 hasConcept C95444343 @default.
- W2160052005 hasConceptScore W2160052005C104317684 @default.
- W2160052005 hasConceptScore W2160052005C107824862 @default.
- W2160052005 hasConceptScore W2160052005C12554922 @default.
- W2160052005 hasConceptScore W2160052005C1491633281 @default.
- W2160052005 hasConceptScore W2160052005C159654299 @default.
- W2160052005 hasConceptScore W2160052005C185592680 @default.
- W2160052005 hasConceptScore W2160052005C25166345 @default.
- W2160052005 hasConceptScore W2160052005C28406088 @default.
- W2160052005 hasConceptScore W2160052005C51639874 @default.
- W2160052005 hasConceptScore W2160052005C54009773 @default.
- W2160052005 hasConceptScore W2160052005C54355233 @default.
- W2160052005 hasConceptScore W2160052005C55493867 @default.
- W2160052005 hasConceptScore W2160052005C85789140 @default.
- W2160052005 hasConceptScore W2160052005C86803240 @default.
- W2160052005 hasConceptScore W2160052005C95444343 @default.
- W2160052005 hasIssue "48" @default.
- W2160052005 hasLocation W21600520051 @default.