Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160067300> ?p ?o ?g. }
- W2160067300 endingPage "3458" @default.
- W2160067300 startingPage "3445" @default.
- W2160067300 abstract "Application of hydrodynamic mild shear stress to adherent Dictyostelium discoideum vegetative cells triggers active actin cytoskeleton remodeling resulting in net cell movement along the flow. The average cell speed is strongly stimulated by external calcium (Ca2+, K50%=22 microM), but the directionality of the movement is almost unaffected. This calcium concentration is ten times higher than the one promoting cell adhesion to glass surfaces (K50%=2 microM). Addition of the calcium chelator EGTA or the Ca2+-channel blocker gadolinium (Gd3+) transiently stops cell movement. Monitoring the evolution of cell-surface contact area with time reveals that calcium stimulates cell speed by increasing the amplitude of both protrusion and retraction events at the cell edge, but not the frequency. As a consequence, with saturating external calcium concentrations, cells are sensitive to very low shear forces (20 pN; sigma=0.1 Pa). Moreover, a null-mutant lacking the unique Gbeta subunit does not respond to external Ca2+ changes (K50%>1000 microM), although the directionality of the movement is comparable with that of wild-type cells. Furthermore, cells lacking the inositol 1,4,5-trisphosphate receptor (IP3-receptor) exhibit a markedly reduced Ca2+ sensitivity. Thus, calcium release from internal stores and calcium entry through the plasma membrane modulate cell speed in response to shear stress." @default.
- W2160067300 created "2016-06-24" @default.
- W2160067300 creator A5005601279 @default.
- W2160067300 creator A5030631651 @default.
- W2160067300 creator A5042868602 @default.
- W2160067300 creator A5053608225 @default.
- W2160067300 creator A5065947624 @default.
- W2160067300 creator A5066166065 @default.
- W2160067300 creator A5069744472 @default.
- W2160067300 creator A5071879346 @default.
- W2160067300 creator A5091366547 @default.
- W2160067300 date "2005-08-01" @default.
- W2160067300 modified "2023-10-14" @default.
- W2160067300 title "Calcium mobilization stimulates<i>Dictyostelium discoideum</i>shear-flow-induced cell motility" @default.
- W2160067300 cites W1533463539 @default.
- W2160067300 cites W1626382598 @default.
- W2160067300 cites W188520909 @default.
- W2160067300 cites W1898695366 @default.
- W2160067300 cites W1936115228 @default.
- W2160067300 cites W1964145620 @default.
- W2160067300 cites W1979111381 @default.
- W2160067300 cites W1981562007 @default.
- W2160067300 cites W1991914681 @default.
- W2160067300 cites W1994583315 @default.
- W2160067300 cites W1996675613 @default.
- W2160067300 cites W2006912278 @default.
- W2160067300 cites W2012023810 @default.
- W2160067300 cites W2014304134 @default.
- W2160067300 cites W2023193403 @default.
- W2160067300 cites W2025882607 @default.
- W2160067300 cites W2029193532 @default.
- W2160067300 cites W2031183644 @default.
- W2160067300 cites W2035474045 @default.
- W2160067300 cites W2037865364 @default.
- W2160067300 cites W2038070469 @default.
- W2160067300 cites W2038940222 @default.
- W2160067300 cites W2058449890 @default.
- W2160067300 cites W2058861059 @default.
- W2160067300 cites W2060317499 @default.
- W2160067300 cites W2067917704 @default.
- W2160067300 cites W2070687879 @default.
- W2160067300 cites W2073326896 @default.
- W2160067300 cites W2073584744 @default.
- W2160067300 cites W2079270430 @default.
- W2160067300 cites W2079944376 @default.
- W2160067300 cites W2095892169 @default.
- W2160067300 cites W2098349937 @default.
- W2160067300 cites W2105503408 @default.
- W2160067300 cites W2112609276 @default.
- W2160067300 cites W2115928735 @default.
- W2160067300 cites W2119836020 @default.
- W2160067300 cites W2124864193 @default.
- W2160067300 cites W2125782986 @default.
- W2160067300 cites W2126496496 @default.
- W2160067300 cites W2141075109 @default.
- W2160067300 cites W2141932305 @default.
- W2160067300 cites W2150181623 @default.
- W2160067300 cites W2153056301 @default.
- W2160067300 cites W2157163814 @default.
- W2160067300 cites W2422979828 @default.
- W2160067300 cites W94487769 @default.
- W2160067300 doi "https://doi.org/10.1242/jcs.02461" @default.
- W2160067300 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16079287" @default.
- W2160067300 hasPublicationYear "2005" @default.
- W2160067300 type Work @default.
- W2160067300 sameAs 2160067300 @default.
- W2160067300 citedByCount "45" @default.
- W2160067300 countsByYear W21600673002012 @default.
- W2160067300 countsByYear W21600673002013 @default.
- W2160067300 countsByYear W21600673002014 @default.
- W2160067300 countsByYear W21600673002015 @default.
- W2160067300 countsByYear W21600673002016 @default.
- W2160067300 countsByYear W21600673002017 @default.
- W2160067300 countsByYear W21600673002018 @default.
- W2160067300 countsByYear W21600673002021 @default.
- W2160067300 countsByYear W21600673002022 @default.
- W2160067300 countsByYear W21600673002023 @default.
- W2160067300 crossrefType "journal-article" @default.
- W2160067300 hasAuthorship W2160067300A5005601279 @default.
- W2160067300 hasAuthorship W2160067300A5030631651 @default.
- W2160067300 hasAuthorship W2160067300A5042868602 @default.
- W2160067300 hasAuthorship W2160067300A5053608225 @default.
- W2160067300 hasAuthorship W2160067300A5065947624 @default.
- W2160067300 hasAuthorship W2160067300A5066166065 @default.
- W2160067300 hasAuthorship W2160067300A5069744472 @default.
- W2160067300 hasAuthorship W2160067300A5071879346 @default.
- W2160067300 hasAuthorship W2160067300A5091366547 @default.
- W2160067300 hasBestOaLocation W21600673001 @default.
- W2160067300 hasConcept C104317684 @default.
- W2160067300 hasConcept C12554922 @default.
- W2160067300 hasConcept C142669718 @default.
- W2160067300 hasConcept C1491633281 @default.
- W2160067300 hasConcept C191897082 @default.
- W2160067300 hasConcept C192562407 @default.
- W2160067300 hasConcept C2775969001 @default.
- W2160067300 hasConcept C2777658833 @default.
- W2160067300 hasConcept C519063684 @default.
- W2160067300 hasConcept C55493867 @default.