Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160128169> ?p ?o ?g. }
- W2160128169 endingPage "130" @default.
- W2160128169 startingPage "119" @default.
- W2160128169 abstract "Drosophila melanogaster embryos are a source for homogeneous and stable 26S proteasomes suitable for structural studies. For biochemical characterization, purified 26S proteasomes were resolved by two-dimensional (2D) gel electrophoresis and subunits composing the regulatory complex (RC) were identified by amino acid sequencing and immunoblotting, before corresponding cDNAs were sequenced. 17 subunits from Drosophila RCs were found to have homologues in the yeast and human RCs. An additional subunit, p37A, not yet described in RCs of other organisms, is a member of the ubiquitin COOH-terminal hydrolase family (UCH). Analysis of EM images of 26S proteasomes-UCH-inhibitor complexes allowed for the first time to localize one of the RC's specific functions, deubiquitylating activity. The masses of 26S proteasomes with either one or two attached RCs were determined by scanning transmission EM (STEM), yielding a mass of 894 kD for a single RC. This value is in good agreement with the summed masses of the 18 identified RC subunits (932 kD), indicating that the number of subunits is complete." @default.
- W2160128169 created "2016-06-24" @default.
- W2160128169 creator A5016116496 @default.
- W2160128169 creator A5020701613 @default.
- W2160128169 creator A5025633573 @default.
- W2160128169 creator A5029559203 @default.
- W2160128169 creator A5033550097 @default.
- W2160128169 creator A5047459030 @default.
- W2160128169 creator A5052940235 @default.
- W2160128169 creator A5060187574 @default.
- W2160128169 creator A5068160772 @default.
- W2160128169 creator A5072884258 @default.
- W2160128169 creator A5088377011 @default.
- W2160128169 date "2000-07-10" @default.
- W2160128169 modified "2023-10-12" @default.
- W2160128169 title "The Regulatory Complex of <i>Drosophila melanogaster</i> 26s Proteasomes" @default.
- W2160128169 cites W141648157 @default.
- W2160128169 cites W148769332 @default.
- W2160128169 cites W1496897161 @default.
- W2160128169 cites W1515490675 @default.
- W2160128169 cites W1518640128 @default.
- W2160128169 cites W1530611492 @default.
- W2160128169 cites W1532285380 @default.
- W2160128169 cites W1532810559 @default.
- W2160128169 cites W1566348916 @default.
- W2160128169 cites W1606746692 @default.
- W2160128169 cites W1840515050 @default.
- W2160128169 cites W1873199501 @default.
- W2160128169 cites W1954801073 @default.
- W2160128169 cites W1967074928 @default.
- W2160128169 cites W1972220674 @default.
- W2160128169 cites W1973904343 @default.
- W2160128169 cites W1978135217 @default.
- W2160128169 cites W1980534130 @default.
- W2160128169 cites W1981522273 @default.
- W2160128169 cites W1981916517 @default.
- W2160128169 cites W1982434218 @default.
- W2160128169 cites W1985728179 @default.
- W2160128169 cites W1989047270 @default.
- W2160128169 cites W1991930087 @default.
- W2160128169 cites W1992671721 @default.
- W2160128169 cites W1994185269 @default.
- W2160128169 cites W1996218556 @default.
- W2160128169 cites W1996460413 @default.
- W2160128169 cites W2003836768 @default.
- W2160128169 cites W2003849992 @default.
- W2160128169 cites W2003924932 @default.
- W2160128169 cites W2004557152 @default.
- W2160128169 cites W2006986747 @default.
- W2160128169 cites W2008744347 @default.
- W2160128169 cites W2018289835 @default.
- W2160128169 cites W2019540943 @default.
- W2160128169 cites W2024645073 @default.
- W2160128169 cites W2038751264 @default.
- W2160128169 cites W2041127974 @default.
- W2160128169 cites W2042129026 @default.
- W2160128169 cites W2053026504 @default.
- W2160128169 cites W2066284211 @default.
- W2160128169 cites W2071473164 @default.
- W2160128169 cites W2072163638 @default.
- W2160128169 cites W2078706180 @default.
- W2160128169 cites W2080063823 @default.
- W2160128169 cites W2081892455 @default.
- W2160128169 cites W2083230754 @default.
- W2160128169 cites W2083838127 @default.
- W2160128169 cites W2085474307 @default.
- W2160128169 cites W2089407625 @default.
- W2160128169 cites W2096913764 @default.
- W2160128169 cites W2097382368 @default.
- W2160128169 cites W2100837269 @default.
- W2160128169 cites W2117118394 @default.
- W2160128169 cites W2123095711 @default.
- W2160128169 cites W2123781000 @default.
- W2160128169 cites W2144593863 @default.
- W2160128169 cites W2147065485 @default.
- W2160128169 cites W2152424535 @default.
- W2160128169 cites W2155885530 @default.
- W2160128169 cites W2160927584 @default.
- W2160128169 cites W2166987747 @default.
- W2160128169 cites W2178503716 @default.
- W2160128169 cites W4211243832 @default.
- W2160128169 cites W4255738047 @default.
- W2160128169 cites W44163030 @default.
- W2160128169 cites W83512084 @default.
- W2160128169 cites W2072417633 @default.
- W2160128169 doi "https://doi.org/10.1083/jcb.150.1.119" @default.
- W2160128169 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2185576" @default.
- W2160128169 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10893261" @default.
- W2160128169 hasPublicationYear "2000" @default.
- W2160128169 type Work @default.
- W2160128169 sameAs 2160128169 @default.
- W2160128169 citedByCount "138" @default.
- W2160128169 countsByYear W21601281692012 @default.
- W2160128169 countsByYear W21601281692013 @default.
- W2160128169 countsByYear W21601281692014 @default.
- W2160128169 countsByYear W21601281692015 @default.
- W2160128169 countsByYear W21601281692016 @default.
- W2160128169 countsByYear W21601281692017 @default.