FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160181611> ?p ?o ?g. }

• W2160181611 endingPage "64" @default.
• W2160181611 startingPage "55" @default.
• W2160181611 abstract "Summary Yadav, R. C., A. Sharma, N. Garg & G. N. Purohit, 2008. Survival of vitrified water buffalo cumulus oocyte complexes and their subsequent development in vitro. Bulg. J. Vet. Med. , 11, No 1, 55 64. The study aimed to determine the effects of different concentrations of glycerol and ethylene glycol and ultrarapid cryopreservation (vitrification) on survival and subsequent in vitro development of bubaline cumulus oocyte complexes (COCs) in order to recognize the optimum cryoprotectant. Survival and in vitro maturation, fertilization and cleavage of buffalo COCs was evaluated subsequent to their cryopreservation by vitrification. The vitrification solution (VS) consisted of Dulbecco’s phosphate buffered saline (DPBS) supplemented with 0.5 M sucrose, 0.5% bovine serum albumin (BSA) and different molar (M) concentrations of the cryoprotectants glycerol (G) (4 M, 6 M, 8 M and 10 M), ethylene glycol (EG) (4 M, 6 M, 8 M and 10 M) and their combinations (2 M G + 2 M EG, 3 M G + 3 M EG, 4 M G + 4 M EG and 5 M G + 5 M EG). The COCs were pre-equilibrated in 50% of the VS for 35 min, then kept in VS for 1 min and loaded in pre-sterilized 0.25 mL semen straws. After 7 10 days of storage COCs were warmed (38 o C for 5 s) and evaluated for morphological damage. Morphologically normal COCs were cultured in vitro and evaluated for nuclear maturation (n=847), fertilization (n=621) and cleavage (n=1451) in two separate experiments. The survival of oocytes was 86.4% and 89.6% in experiment 1 and 2. The highest proportion of normal oocytes was seen in 6 M EG and the lowest  in 10 M G in both experiments. The in vitro maturation of oocytes at the end of experiment 1, and the in vitro fertilization and cleavage at the end of experiment 2, were significantly lower in all tested vitrification cryoprotectants compared to control. A dose-dependant increase in the proportion of oocytes matured, fertilized or cleaved was seen for both G and EG up to concentrations of 8 M. There was no specific benefit of combining G and EG on the subsequent in vitro maturation, fertilization and cleavage of oocytes. At equal concentrations EG proved to be a better cryoprotectant than G. It was concluded that there appeared to be a limit to the concentration of the cryoprotectant (8 M) beyond which the cryoprotectant exerted suboptimal effects and that there was no benefit of combining two similar cryoprotectants for cryopreservation of oocytes by vitrification." @default.
• W2160181611 created "2016-06-24" @default.
• W2160181611 creator A5029416116 @default.
• W2160181611 creator A5032596176 @default.
• W2160181611 creator A5035953110 @default.
• W2160181611 creator A5076587837 @default.
• W2160181611 date "2008-01-01" @default.
• W2160181611 modified "2023-09-26" @default.
• W2160181611 title "SURVIVAL OF VITRIFIED WATER BUFFALO CUMULUS OOCYTE COMPLEXES AND THEIR SUBSEQUENT DEVELOPMENT IN VITRO" @default.
• W2160181611 cites W1545776712 @default.
• W2160181611 cites W1899400872 @default.
• W2160181611 cites W1965413255 @default.
• W2160181611 cites W1966144272 @default.
• W2160181611 cites W1976339009 @default.
• W2160181611 cites W1982700421 @default.
• W2160181611 cites W1988288310 @default.
• W2160181611 cites W1988355952 @default.
• W2160181611 cites W1991693360 @default.
• W2160181611 cites W1995086023 @default.
• W2160181611 cites W1998610434 @default.
• W2160181611 cites W2008195023 @default.
• W2160181611 cites W2009551466 @default.
• W2160181611 cites W2016901731 @default.
• W2160181611 cites W2021062181 @default.
• W2160181611 cites W2028931781 @default.
• W2160181611 cites W2040160244 @default.
• W2160181611 cites W2042955599 @default.
• W2160181611 cites W2044159648 @default.
• W2160181611 cites W2046836003 @default.
• W2160181611 cites W2051478911 @default.
• W2160181611 cites W2055624916 @default.
• W2160181611 cites W2069329287 @default.
• W2160181611 cites W2112077845 @default.
• W2160181611 cites W2112392835 @default.
• W2160181611 cites W2118707841 @default.
• W2160181611 cites W2128572589 @default.
• W2160181611 cites W2131391649 @default.
• W2160181611 cites W2155255557 @default.
• W2160181611 cites W2157480432 @default.
• W2160181611 cites W2163927849 @default.
• W2160181611 cites W2230872914 @default.
• W2160181611 cites W2280988113 @default.
• W2160181611 cites W2319558413 @default.
• W2160181611 cites W2341936148 @default.
• W2160181611 hasPublicationYear "2008" @default.
• W2160181611 type Work @default.
• W2160181611 sameAs 2160181611 @default.
• W2160181611 citedByCount "7" @default.
• W2160181611 countsByYear W21601816112012 @default.
• W2160181611 countsByYear W21601816112014 @default.
• W2160181611 countsByYear W21601816112015 @default.
• W2160181611 crossrefType "journal-article" @default.
• W2160181611 hasAuthorship W2160181611A5029416116 @default.
• W2160181611 hasAuthorship W2160181611A5032596176 @default.
• W2160181611 hasAuthorship W2160181611A5035953110 @default.
• W2160181611 hasAuthorship W2160181611A5076587837 @default.
• W2160181611 hasConcept C104317684 @default.
• W2160181611 hasConcept C105702510 @default.
• W2160181611 hasConcept C140793950 @default.
• W2160181611 hasConcept C16685009 @default.
• W2160181611 hasConcept C178790620 @default.
• W2160181611 hasConcept C179933525 @default.
• W2160181611 hasConcept C185592680 @default.
• W2160181611 hasConcept C196843134 @default.
• W2160181611 hasConcept C2775966511 @default.
• W2160181611 hasConcept C2776690073 @default.
• W2160181611 hasConcept C2777516009 @default.
• W2160181611 hasConcept C2778022349 @default.
• W2160181611 hasConcept C2778177303 @default.
• W2160181611 hasConcept C2780881558 @default.
• W2160181611 hasConcept C505870484 @default.
• W2160181611 hasConcept C512716672 @default.
• W2160181611 hasConcept C55493867 @default.
• W2160181611 hasConcept C68528507 @default.
• W2160181611 hasConcept C71924100 @default.
• W2160181611 hasConcept C86803240 @default.
• W2160181611 hasConcept C87073359 @default.
• W2160181611 hasConcept C88972607 @default.
• W2160181611 hasConceptScore W2160181611C104317684 @default.
• W2160181611 hasConceptScore W2160181611C105702510 @default.
• W2160181611 hasConceptScore W2160181611C140793950 @default.
• W2160181611 hasConceptScore W2160181611C16685009 @default.
• W2160181611 hasConceptScore W2160181611C178790620 @default.
• W2160181611 hasConceptScore W2160181611C179933525 @default.
• W2160181611 hasConceptScore W2160181611C185592680 @default.
• W2160181611 hasConceptScore W2160181611C196843134 @default.
• W2160181611 hasConceptScore W2160181611C2775966511 @default.
• W2160181611 hasConceptScore W2160181611C2776690073 @default.
• W2160181611 hasConceptScore W2160181611C2777516009 @default.
• W2160181611 hasConceptScore W2160181611C2778022349 @default.
• W2160181611 hasConceptScore W2160181611C2778177303 @default.
• W2160181611 hasConceptScore W2160181611C2780881558 @default.
• W2160181611 hasConceptScore W2160181611C505870484 @default.
• W2160181611 hasConceptScore W2160181611C512716672 @default.
• W2160181611 hasConceptScore W2160181611C55493867 @default.
• W2160181611 hasConceptScore W2160181611C68528507 @default.
• W2160181611 hasConceptScore W2160181611C71924100 @default.
• W2160181611 hasConceptScore W2160181611C86803240 @default.

• next