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• W2160202281 startingPage "1043" @default.
• W2160202281 abstract "This paper reports the design, fabrication and characterization of a cell stretching device based on the side stretching approach. Numerical simulation using finite element method provides a guideline for optimizing the geometry and maximizing the output strain of the stretched membrane. An unique PDMS-based micro fabrication process was developed for obtaining high parallelization, well controlled membrane thickness and an ultra-thin bottom layer that is crucial for the use with confocal microscopes. The stretching experiments are fully automated with both device actuation and image acquisition. A programmable pneumatic control system was built for simultaneous driving of 24 stretching arrays. The actuation signals are synchronized with the image acquisition system to obtain time-lapse recording of cells grown on the stretched membrane. Experimental results verified the characteristics predicted by the simulation. A platform with 15 stretching units was integrated on a standard 24 mm × 50 mm glass slide. Each unit can achieve a maximum strain of more than 60 %. The platform was tested for cell growth under cyclic stretching. The preliminary results show that the device is compatible with all standard microscopes." @default.
• W2160202281 created "2016-06-24" @default.
• W2160202281 creator A5012833011 @default.
• W2160202281 creator A5049097498 @default.
• W2160202281 date "2013-07-19" @default.
• W2160202281 modified "2023-10-10" @default.
• W2160202281 title "A polymeric cell stretching device for real-time imaging with optical microscopy" @default.
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• W2160202281 doi "https://doi.org/10.1007/s10544-013-9796-2" @default.
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• W2160202281 hasPublicationYear "2013" @default.
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