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- W2160306862 abstract "The thermoacidophilic archaeon Sulfolobus solfataricus MT4 encodes a maltooligosyltrehalose synthase (MTS), that catalyzes an intramolecular transglycosylation process converting the glycosidic linkages at the reducing end of dextrins from α-1,4 into α-1,1. In this research the gene encoding MTS was cloned and expressed in Lactococcus lactis NZ9000 using the so-called NICE system. Growth conditions of the recombinant strain were optimized in flask experiments in relation to enzyme production. Batch experiments in 2 L-fermenters were performed on the best identified semidefined medium and 256 U L−1 of recombinant MTS were produced. Purified recombinant MTS shows its optimal activity at 70 °C and pH 5.5, prefers maltoheptaose and maltohexaose as substrates, and demonstrates minimal side hydrolytic activity." @default.
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- W2160306862 date "2008-07-02" @default.
- W2160306862 modified "2023-09-25" @default.
- W2160306862 title "Production of a thermophilic maltooligosyl-trehalose synthase in Lactococcus lactis" @default.
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- W2160306862 doi "https://doi.org/10.1007/s10295-008-0384-z" @default.
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