Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160330200> ?p ?o ?g. }
- W2160330200 endingPage "79" @default.
- W2160330200 startingPage "69" @default.
- W2160330200 abstract "Recently we reported the presence of bacteria within follicular fluid. Previous studies have reported that DNA fragmentation in human spermatozoa after in vivo or in vitro incubation with bacteria results in early embryo demise and a reduced rate of ongoing pregnancy, but the effect of bacteria on oocytes is unknown. This study examined the DNA within mouse oocytes after 12 hours' incubation within human follicular fluids (n=5), which were collected from women undergoing in vitro fertilization (IVF) treatment. Each follicular fluid sample was cultured to detect the presence of bacteria. Terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) was used to label DNA fragmentation in ovulated, non-fertilized mouse oocytes following in vitro incubation in human follicular fluid. The bacteria Streptococcus anginosus and Peptoniphilus spp., Lactobacillus gasseri (low-dose), L. gasseri (high-dose), Enterococcus faecalis, or Propionibacterium acnes were detected within the follicular fluids. The most severe DNA fragmentation was observed in oocytes incubated in the follicular fluids containing P. acnes or L. gasseri (high-dose). No DNA fragmentation was observed in the mouse oocytes incubated in the follicular fluid containing low-dose L. gasseri or E. faecalis. Low human oocyte fertilization rates (<29%) were associated with extensive fragmentation in mouse oocytes (80-100%). Bacteria colonizing human follicular fluid in vivo may cause DNA fragmentation in mouse oocytes following 12h of in vitro incubation. Follicular fluid bacteria may result in poor quality oocytes and/or embryos, leading to poor IVF outcomes." @default.
- W2160330200 created "2016-06-24" @default.
- W2160330200 creator A5018576672 @default.
- W2160330200 creator A5038409288 @default.
- W2160330200 creator A5046413422 @default.
- W2160330200 creator A5063776396 @default.
- W2160330200 creator A5069576740 @default.
- W2160330200 creator A5072950358 @default.
- W2160330200 creator A5079803621 @default.
- W2160330200 date "2013-09-01" @default.
- W2160330200 modified "2023-10-10" @default.
- W2160330200 title "TUNEL analysis of DNA fragmentation in mouse unfertilized oocytes: The effect of microorganisms within human follicular fluid collected during IVF cycles" @default.
- W2160330200 cites W143757948 @default.
- W2160330200 cites W1965397129 @default.
- W2160330200 cites W1971842547 @default.
- W2160330200 cites W1974424681 @default.
- W2160330200 cites W1976299761 @default.
- W2160330200 cites W1985934519 @default.
- W2160330200 cites W1986659373 @default.
- W2160330200 cites W1988980234 @default.
- W2160330200 cites W1991754956 @default.
- W2160330200 cites W1991760369 @default.
- W2160330200 cites W1992915927 @default.
- W2160330200 cites W1993158891 @default.
- W2160330200 cites W1997800433 @default.
- W2160330200 cites W2000261392 @default.
- W2160330200 cites W2005111721 @default.
- W2160330200 cites W2014976628 @default.
- W2160330200 cites W2022270574 @default.
- W2160330200 cites W2029261081 @default.
- W2160330200 cites W2032704919 @default.
- W2160330200 cites W2047912510 @default.
- W2160330200 cites W2049693640 @default.
- W2160330200 cites W2060395795 @default.
- W2160330200 cites W2073651068 @default.
- W2160330200 cites W2075479850 @default.
- W2160330200 cites W2078446580 @default.
- W2160330200 cites W2081379553 @default.
- W2160330200 cites W2083055659 @default.
- W2160330200 cites W2086145231 @default.
- W2160330200 cites W2094904331 @default.
- W2160330200 cites W2107580100 @default.
- W2160330200 cites W2122215451 @default.
- W2160330200 cites W2125462620 @default.
- W2160330200 cites W2125585441 @default.
- W2160330200 cites W2128090435 @default.
- W2160330200 cites W2128769733 @default.
- W2160330200 cites W2142026735 @default.
- W2160330200 cites W2142929121 @default.
- W2160330200 cites W2144457833 @default.
- W2160330200 cites W2145224687 @default.
- W2160330200 cites W2153256987 @default.
- W2160330200 cites W2158518415 @default.
- W2160330200 cites W2160272767 @default.
- W2160330200 cites W2164843552 @default.
- W2160330200 cites W2167120509 @default.
- W2160330200 cites W2168774785 @default.
- W2160330200 cites W2172104309 @default.
- W2160330200 cites W2297703860 @default.
- W2160330200 cites W2337937543 @default.
- W2160330200 cites W4241342955 @default.
- W2160330200 doi "https://doi.org/10.1016/j.jri.2013.07.004" @default.
- W2160330200 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23972717" @default.
- W2160330200 hasPublicationYear "2013" @default.
- W2160330200 type Work @default.
- W2160330200 sameAs 2160330200 @default.
- W2160330200 citedByCount "12" @default.
- W2160330200 countsByYear W21603302002015 @default.
- W2160330200 countsByYear W21603302002016 @default.
- W2160330200 countsByYear W21603302002017 @default.
- W2160330200 countsByYear W21603302002020 @default.
- W2160330200 countsByYear W21603302002022 @default.
- W2160330200 countsByYear W21603302002023 @default.
- W2160330200 crossrefType "journal-article" @default.
- W2160330200 hasAuthorship W2160330200A5018576672 @default.
- W2160330200 hasAuthorship W2160330200A5038409288 @default.
- W2160330200 hasAuthorship W2160330200A5046413422 @default.
- W2160330200 hasAuthorship W2160330200A5063776396 @default.
- W2160330200 hasAuthorship W2160330200A5069576740 @default.
- W2160330200 hasAuthorship W2160330200A5072950358 @default.
- W2160330200 hasAuthorship W2160330200A5079803621 @default.
- W2160330200 hasConcept C134018914 @default.
- W2160330200 hasConcept C153911025 @default.
- W2160330200 hasConcept C16685009 @default.
- W2160330200 hasConcept C187785154 @default.
- W2160330200 hasConcept C18903297 @default.
- W2160330200 hasConcept C190283241 @default.
- W2160330200 hasConcept C191015642 @default.
- W2160330200 hasConcept C196843134 @default.
- W2160330200 hasConcept C2776188440 @default.
- W2160330200 hasConcept C2776690073 @default.
- W2160330200 hasConcept C31573885 @default.
- W2160330200 hasConcept C43759708 @default.
- W2160330200 hasConcept C54355233 @default.
- W2160330200 hasConcept C55493867 @default.
- W2160330200 hasConcept C71924100 @default.
- W2160330200 hasConcept C86803240 @default.
- W2160330200 hasConcept C89423630 @default.