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• W2160443407 endingPage "2830" @default.
• W2160443407 startingPage "2823" @default.
• W2160443407 abstract "ABSTRACT During respiratory glucose dissimilation, eukaryotes produce cytosolic NADH via glycolysis. This NADH has to be reoxidized outside the mitochondria, because the mitochondrial inner membrane is impermeable to NADH. In Saccharomyces cerevisiae , this may involve external NADH dehydrogenases (Nde1p or Nde2p) and/or a glycerol-3-phosphate shuttle consisting of soluble (Gpd1p or Gpd2p) and membrane-bound (Gut2p) glycerol-3-phosphate dehydrogenases. This study addresses the physiological relevance of these mechanisms and the possible involvement of alternative routes for mitochondrial oxidation of cytosolic NADH. Aerobic, glucose-limited chemostat cultures of a gut2Δ mutant exhibited fully respiratory growth at low specific growth rates. Alcoholic fermentation set in at the same specific growth rate as in wild-type cultures (0.3 h −1 ). Apparently, the glycerol-3-phosphate shuttle is not essential for respiratory glucose dissimilation. An nde1Δ nde2Δ mutant already produced glycerol at specific growth rates of 0.10 h −1 and above, indicating a requirement for external NADH dehydrogenase to sustain fully respiratory growth. An nde1Δ nde2Δ gut2Δ mutant produced even larger amounts of glycerol at specific growth rates ranging from 0.05 to 0.15 h −1 . Apparently, even at a low glycolytic flux, alternative mechanisms could not fully replace the external NADH dehydrogenases and glycerol-3-phosphate shuttle. However, at low dilution rates, the nde1Δ nde2Δ gut2Δ mutant did not produce ethanol. Since glycerol production could not account for all glycolytic NADH, another NADH-oxidizing system has to be present. Two alternative mechanisms for reoxidizing cytosolic NADH are discussed: (i) cytosolic production of ethanol followed by its intramitochondrial oxidation and (ii) a redox shuttle linking cytosolic NADH oxidation to the internal NADH dehydrogenase." @default.
• W2160443407 created "2016-06-24" @default.
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• W2160443407 date "2000-05-15" @default.
• W2160443407 modified "2023-10-16" @default.
• W2160443407 title "In Vivo Analysis of the Mechanisms for Oxidation of Cytosolic NADH by <i>Saccharomyces cerevisiae</i> Mitochondria" @default.
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• W2160443407 doi "https://doi.org/10.1128/jb.182.10.2823-2830.2000" @default.
• W2160443407 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/101991" @default.
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