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• W2160565208 abstract "Anthranilate synthase (EC 4.1.3.27) has been purified from cell cultures of Catharanthus roseus by poly(ethylene glycol) precipitation/fractionation and subsequent separation by anion exchange on Q-Sepharose, Orange A dye chromatography, Mono Q anion-exchange chromatography and Superose 6 gel filtration. By analogy to anthranilate synthases from other sources it does look like the enzyme is a tetramer composed of two large and two small subunits, with molecular mass 67 and 25.5±0.5 kDa, respectively. The molecular mass determined by gel filtration was 143±5 kDa. The enzyme had a pI of 5.1 determined by chromatofocusing. The pH optimum was between pH 7.5 and pH 8.3, but the type of buffer used affected the results. The enzyme could utilize NH4+ as ammonium donor instead of glutamine. The enzyme showed normal Michaelis-Menten kinetics with respect to the substrates l-glutamine and chorismate, and the cofactor Mg2+, Km values for l-glutamine was determined to be 0.37±0.05 mM, for chorismate 67±3 μM, and for MgCl2 0.26±0.03 mM respectively. Anthranilate synthase was inhibited by l-tryptophan, tryptamine and d-tryptophan (with l-tryptophan being the best inhibitor). The enzyme was allosterically regulated showing positive cooperativity of chorismate binding at higher concentrations of tryptophan. For a tryptophan concentration of 20 μM the Hill coefficient was determined to be 2. The tryptophan binding sites showed positive cooperativity for higher concentrations of chorismate. The purified enzyme did not contain anthranilate-5-phosphoribosylpyrophosphate phosphoribosyltransferase activity and is thus not of the same type as the well characterized Salmonella typhimurium anthranilate synthase/phosphoribosyl pyrophosphate transferase bifunctional type." @default.
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• W2160565208 creator A5022333147 @default.
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• W2160565208 date "1993-03-01" @default.
• W2160565208 modified "2023-10-16" @default.
• W2160565208 title "Purification and characterization of anthranilate synthase from Catharanthus roseus" @default.
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• W2160565208 doi "https://doi.org/10.1111/j.1432-1033.1993.tb17679.x" @default.
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