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- W2160604128 abstract "Synthesized gene of human thymosin alpha 1 (Talpha1) was inserted into pET-28a, pET-9c, pThioHis B, pGEX-2T or pBV222 and then inductively expressed in strains of Escherichia coli. Among the five expression systems, the BL21/pET-28a system provides the highest expression level of fusion protein in a soluble form, which is up to 70% of total expressed bacterial proteins as visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The resulting fusion protein purified through nickel affinity chromatography accounts for 2.53% of the wet bacterial pellet weight and reaches 94.5% purity by SDS-PAGE. These results indicate the potential of this expression system for high-throughput production of recombinant Talpha1." @default.
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- W2160604128 date "2005-02-01" @default.
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- W2160604128 title "Overexpression of Soluble Human Thymosin Alpha 1 in <italic>Escherichia coli</italic>" @default.
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- W2160604128 doi "https://doi.org/10.1093/abbs/37.2.147" @default.
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