FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160669067> ?p ?o ?g. }

• W2160669067 endingPage "791" @default.
• W2160669067 startingPage "785" @default.
• W2160669067 abstract "Embryos produced by in-vitro fertilization (IVF) may produce less platelet-activating factor (PAF) than is optimal for development. It was previously shown that supplementation of culture media with PAF results in a significant increase in pregnancy rate. Human embryos are often cultured in media supplemented with serum containing the enzyme PAF:acetylhydrolase (PAF:AH; EC 3.1.1.47), which hydrolyses PAF to its inactive form, lyso-PAF. Thus, effective supplementation of media with PAF requires inactivation of this enzyme. In this study we examine the efficacy of the methods of PAF:AH deactivation used for PAF supplementation of IVF culture medium. When the effectiveness of a commonly used acid treatment protocol (pH 3.0 at room temperature for 5 min) was examined, it was found that it was not completely effective for the majority of sera. When synthetic PAF was added to 18 serum samples which had been acid treated, five had 90-100% of the original PAF remaining after 24 h (showing that the acid treatment was effective), eight had from 10-90% of the original PAF remaining after 24 h, and five samples had 0-10%. The extent to which PAF:AH was susceptible to deactivation was not associated with the activity in the serum prior to treatment, the serum oestradiol concentration, or the cause of infertility. The period of acidification and the incubation temperature were assessed to develop a new acid-treatment protocol (20 min acid treatment at 37 degrees C) which was able to deactivate PAF:AH effectively in all sera (53/53) examined. A trial was performed to assess the effect of acid treatment of serum for 5 min at room temperature compared with the new protocol (20 min at 37 degrees C) on IVF outcome, following PAF supplementation of IVF culture medium. Oocyte recovery, fertilization and embryo development rates were equivalent for both groups and approximately equal numbers of embryos were transferred or cryopreserved. Pregnancy rates were not significantly different (14.6 versus 20.0%) for the two treatments, with a trend towards a higher pregnancy rate with the new acid-treatment protocol. The results show that this new procedure for acid treatment of serum in combination with PAF supplementation does not have detrimental effects on embryos and their pregnancy outcome and is therefore suitable for use in IVF." @default.
• W2160669067 created "2016-06-24" @default.
• W2160669067 creator A5035955986 @default.
• W2160669067 creator A5068450777 @default.
• W2160669067 date "1997-04-01" @default.
• W2160669067 modified "2023-09-26" @default.
• W2160669067 title "Optimization of a method for deactivation of platelet-activating factor:acetylhydrolase in serum for use in in-vitro fertilization culture media" @default.
• W2160669067 cites W1574747042 @default.
• W2160669067 cites W1902314260 @default.
• W2160669067 cites W2006206565 @default.
• W2160669067 cites W2022527601 @default.
• W2160669067 cites W2023656054 @default.
• W2160669067 cites W2034105269 @default.
• W2160669067 cites W2044000386 @default.
• W2160669067 cites W2046895444 @default.
• W2160669067 cites W2088009524 @default.
• W2160669067 cites W2107098688 @default.
• W2160669067 cites W2152311269 @default.
• W2160669067 cites W2290095822 @default.
• W2160669067 cites W2411317977 @default.
• W2160669067 doi "https://doi.org/10.1093/humrep/12.4.785" @default.
• W2160669067 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9159443" @default.
• W2160669067 hasPublicationYear "1997" @default.
• W2160669067 type Work @default.
• W2160669067 sameAs 2160669067 @default.
• W2160669067 citedByCount "5" @default.
• W2160669067 countsByYear W21606690672013 @default.
• W2160669067 countsByYear W21606690672015 @default.
• W2160669067 crossrefType "journal-article" @default.
• W2160669067 hasAuthorship W2160669067A5035955986 @default.
• W2160669067 hasAuthorship W2160669067A5068450777 @default.
• W2160669067 hasBestOaLocation W21606690671 @default.
• W2160669067 hasConcept C134018914 @default.
• W2160669067 hasConcept C143701427 @default.
• W2160669067 hasConcept C16685009 @default.
• W2160669067 hasConcept C181199279 @default.
• W2160669067 hasConcept C185592680 @default.
• W2160669067 hasConcept C196843134 @default.
• W2160669067 hasConcept C202751555 @default.
• W2160669067 hasConcept C25642318 @default.
• W2160669067 hasConcept C2779020240 @default.
• W2160669067 hasConcept C512716672 @default.
• W2160669067 hasConcept C55493867 @default.
• W2160669067 hasConcept C6557445 @default.
• W2160669067 hasConcept C71924100 @default.
• W2160669067 hasConcept C86803240 @default.
• W2160669067 hasConcept C88972607 @default.
• W2160669067 hasConcept C95444343 @default.
• W2160669067 hasConceptScore W2160669067C134018914 @default.
• W2160669067 hasConceptScore W2160669067C143701427 @default.
• W2160669067 hasConceptScore W2160669067C16685009 @default.
• W2160669067 hasConceptScore W2160669067C181199279 @default.
• W2160669067 hasConceptScore W2160669067C185592680 @default.
• W2160669067 hasConceptScore W2160669067C196843134 @default.
• W2160669067 hasConceptScore W2160669067C202751555 @default.
• W2160669067 hasConceptScore W2160669067C25642318 @default.
• W2160669067 hasConceptScore W2160669067C2779020240 @default.
• W2160669067 hasConceptScore W2160669067C512716672 @default.
• W2160669067 hasConceptScore W2160669067C55493867 @default.
• W2160669067 hasConceptScore W2160669067C6557445 @default.
• W2160669067 hasConceptScore W2160669067C71924100 @default.
• W2160669067 hasConceptScore W2160669067C86803240 @default.
• W2160669067 hasConceptScore W2160669067C88972607 @default.
• W2160669067 hasConceptScore W2160669067C95444343 @default.
• W2160669067 hasIssue "4" @default.
• W2160669067 hasLocation W21606690671 @default.
• W2160669067 hasLocation W21606690672 @default.
• W2160669067 hasOpenAccess W2160669067 @default.
• W2160669067 hasPrimaryLocation W21606690671 @default.
• W2160669067 hasRelatedWork W1122705539 @default.
• W2160669067 hasRelatedWork W1972339110 @default.
• W2160669067 hasRelatedWork W1977589326 @default.
• W2160669067 hasRelatedWork W1986227046 @default.
• W2160669067 hasRelatedWork W2057202558 @default.
• W2160669067 hasRelatedWork W2160669067 @default.
• W2160669067 hasRelatedWork W2406869037 @default.
• W2160669067 hasRelatedWork W2952557449 @default.
• W2160669067 hasRelatedWork W4240174789 @default.
• W2160669067 hasRelatedWork W2612324791 @default.
• W2160669067 hasVolume "12" @default.
• W2160669067 isParatext "false" @default.
• W2160669067 isRetracted "false" @default.
• W2160669067 magId "2160669067" @default.
• W2160669067 workType "article" @default.