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- W2160711929 abstract "ABSTRACT Antibodies against cortisol-21-hemisuccinate conjugated to bovine serum albumin was raised in rabbits and conjugated with poly-maleic acid anhydride. The conjugate was used in a solid phase radioimmunoassay to measure the urinary excretion of unconjugated cortisol. Cortisol was extracted with methylene chloride, the extract purified by chromatography on Sephadex LH-20, and the cortisol content measured with the radioimmunoassay. The intra-assay variation of the method was 6 per cent and the interassay variation 10 per cent. In 95 normal adult persons the excretion of free cortisol was 11–70, mean 30 μg/24 h. No sex or age difference was found. In 8 children aged 7–14 years the excretion was 8–46 μg/24 h." @default.
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- W2160711929 date "1979-08-01" @default.
- W2160711929 modified "2023-10-17" @default.
- W2160711929 title "POLY-MALEIC ACID ANHYDRIDE (PMA) COUPLED CORTISOL ANTIBODY IN ROUTINE DETERMINATION OF URINARY FREE CORTISOL" @default.
- W2160711929 doi "https://doi.org/10.1530/acta.0.0910680" @default.
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