Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160833360> ?p ?o ?g. }
- W2160833360 endingPage "214" @default.
- W2160833360 startingPage "203" @default.
- W2160833360 abstract "Abstract This investigation focuses on transcription factor involvement in blood–brain barrier (BBB) endothelial cell‐induced alterations under conditions of hypoxia and post‐hypoxia/reoxygenation (H/R), using established in vivo / ex vivo and in vitro BBB models. Protein/DNA array analyses revealed a correlation in key transcription factor activation during hypoxia and H/R, including NFκB and hypoxia‐inducible factor (HIF)1. Electrophoretic mobility shift assays confirmed NFκB and HIF1 binding activity ex vivo and in vitro , under conditions of hypoxia and H/R. Hypoxia‐ and H/R‐treated BBB endothelium showed increased HIF1α protein expression in both cytoplasmic and nuclear fractions, in ex vivo and in vitro models. Co‐immunoprecipitation of HIF1α and HIF1β was shown in the nuclear fraction under conditions of hypoxia and H/R in both models. Hypoxia‐ and H/R‐treated BBB endothelium showed increased expression of NFκB‐p65 protein in both cytoplasmic and nuclear fractions. Co‐immunoprecipitation of NFκB‐p65 with NFκB‐p50 was shown in the nuclear fraction under conditions of hypoxia and H/R in the ex vivo model, and after H/R in the in vitro model. These data offer novel avenues in which to alter and/or investigate BBB activity across model systems and to further our understanding of upstream regulators during hypoxia and H/R." @default.
- W2160833360 created "2016-06-24" @default.
- W2160833360 creator A5037975807 @default.
- W2160833360 creator A5038895323 @default.
- W2160833360 creator A5084193351 @default.
- W2160833360 creator A5085069824 @default.
- W2160833360 date "2004-12-09" @default.
- W2160833360 modified "2023-10-14" @default.
- W2160833360 title "Hypoxia-inducible factor and nuclear factor kappa-B activation in blood-brain barrier endothelium under hypoxic/reoxygenation stress" @default.
- W2160833360 cites W1488835155 @default.
- W2160833360 cites W1514513302 @default.
- W2160833360 cites W1516457342 @default.
- W2160833360 cites W1595866873 @default.
- W2160833360 cites W1679229092 @default.
- W2160833360 cites W1962468650 @default.
- W2160833360 cites W1968598354 @default.
- W2160833360 cites W1969024031 @default.
- W2160833360 cites W1969455673 @default.
- W2160833360 cites W1976037108 @default.
- W2160833360 cites W1976541189 @default.
- W2160833360 cites W1978881167 @default.
- W2160833360 cites W1980177300 @default.
- W2160833360 cites W1980654745 @default.
- W2160833360 cites W1980932394 @default.
- W2160833360 cites W1984371193 @default.
- W2160833360 cites W1992116006 @default.
- W2160833360 cites W1993243592 @default.
- W2160833360 cites W1995414864 @default.
- W2160833360 cites W1996537616 @default.
- W2160833360 cites W1997971145 @default.
- W2160833360 cites W1998237791 @default.
- W2160833360 cites W2000040414 @default.
- W2160833360 cites W2000624133 @default.
- W2160833360 cites W2000976163 @default.
- W2160833360 cites W2003194398 @default.
- W2160833360 cites W2009050336 @default.
- W2160833360 cites W2010057452 @default.
- W2160833360 cites W2014171022 @default.
- W2160833360 cites W2024206845 @default.
- W2160833360 cites W2025284114 @default.
- W2160833360 cites W2029606032 @default.
- W2160833360 cites W2031344267 @default.
- W2160833360 cites W2035211789 @default.
- W2160833360 cites W2040895935 @default.
- W2160833360 cites W2049746210 @default.
- W2160833360 cites W2054321620 @default.
- W2160833360 cites W2057652942 @default.
- W2160833360 cites W2071018175 @default.
- W2160833360 cites W2073292880 @default.
- W2160833360 cites W2075054245 @default.
- W2160833360 cites W2077541372 @default.
- W2160833360 cites W2080722863 @default.
- W2160833360 cites W2083913805 @default.
- W2160833360 cites W2087899702 @default.
- W2160833360 cites W2096890135 @default.
- W2160833360 cites W2100516941 @default.
- W2160833360 cites W2100837885 @default.
- W2160833360 cites W2101957440 @default.
- W2160833360 cites W2107440856 @default.
- W2160833360 cites W2107914421 @default.
- W2160833360 cites W2110149504 @default.
- W2160833360 cites W2112390351 @default.
- W2160833360 cites W2117842006 @default.
- W2160833360 cites W2121212243 @default.
- W2160833360 cites W2134542375 @default.
- W2160833360 cites W2135836641 @default.
- W2160833360 cites W2136495845 @default.
- W2160833360 cites W2142786915 @default.
- W2160833360 cites W2163411441 @default.
- W2160833360 cites W2165523086 @default.
- W2160833360 cites W2169265493 @default.
- W2160833360 cites W2171295750 @default.
- W2160833360 cites W2171610971 @default.
- W2160833360 cites W2179556324 @default.
- W2160833360 cites W2188765569 @default.
- W2160833360 cites W2262391931 @default.
- W2160833360 cites W2296856157 @default.
- W2160833360 cites W2326639001 @default.
- W2160833360 cites W2999065750 @default.
- W2160833360 cites W4241897771 @default.
- W2160833360 cites W4379365691 @default.
- W2160833360 doi "https://doi.org/10.1111/j.1471-4159.2004.02871.x" @default.
- W2160833360 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15606909" @default.
- W2160833360 hasPublicationYear "2004" @default.
- W2160833360 type Work @default.
- W2160833360 sameAs 2160833360 @default.
- W2160833360 citedByCount "52" @default.
- W2160833360 countsByYear W21608333602012 @default.
- W2160833360 countsByYear W21608333602013 @default.
- W2160833360 countsByYear W21608333602014 @default.
- W2160833360 countsByYear W21608333602015 @default.
- W2160833360 countsByYear W21608333602016 @default.
- W2160833360 countsByYear W21608333602018 @default.
- W2160833360 countsByYear W21608333602022 @default.
- W2160833360 countsByYear W21608333602023 @default.
- W2160833360 crossrefType "journal-article" @default.
- W2160833360 hasAuthorship W2160833360A5037975807 @default.
- W2160833360 hasAuthorship W2160833360A5038895323 @default.