FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160848082> ?p ?o ?g. }

• W2160848082 endingPage "5425" @default.
• W2160848082 startingPage "5417" @default.
• W2160848082 abstract "Abstract The PspGI restriction–modification system recognizes the sequence CCWGG. R.PspGI cuts DNA before the first C in the cognate sequence and M.PspGI is thought to methylate N4 of one of the cytosines in the sequence. M.PspGI enhances fluorescence of 2-aminopurine in DNA if it replaces the second C in the sequence, while R.PspGI enhances fluorescence when the fluorophore replaces ade-nine in the central base pair. This strongly suggests that the methyltransferase flips the second C in the recognition sequence, while the endonuclease flips both bases in the central base pair out of the duplex. M.PspGI is the first N4-cytosine MTase for which biochemical evidence for base flipping has been presented. It is also the first type IIP methyltransferase whose catalytic activity is strongly stimulated by divalent metal ions. However, divalent metal ions are not required for its base-flipping activity. In contrast, these ions are required for both base flipping and catalysis by the endonuclease. The two enzymes have similar temperature profiles for base flipping and optimal flipping occurs at temperatures substantially below the growth temperature of the source organism for PspGI and for the catalytic activity of endonuclease. We discuss the implications of these results for DNA binding by these enzymes and their evolutionary origin." @default.
• W2160848082 created "2016-06-24" @default.
• W2160848082 creator A5075917155 @default.
• W2160848082 creator A5080092101 @default.
• W2160848082 date "2008-08-20" @default.
• W2160848082 modified "2023-10-13" @default.
• W2160848082 title "DNA base flipping by both members of the PspGI restriction-modification system" @default.
• W2160848082 cites W1579716589 @default.
• W2160848082 cites W1867958153 @default.
• W2160848082 cites W1966800371 @default.
• W2160848082 cites W1986844235 @default.
• W2160848082 cites W1997974036 @default.
• W2160848082 cites W2004517691 @default.
• W2160848082 cites W2018344214 @default.
• W2160848082 cites W2018512856 @default.
• W2160848082 cites W2022673248 @default.
• W2160848082 cites W2034640951 @default.
• W2160848082 cites W2036536166 @default.
• W2160848082 cites W2047232029 @default.
• W2160848082 cites W2054113027 @default.
• W2160848082 cites W2055593712 @default.
• W2160848082 cites W2058743988 @default.
• W2160848082 cites W2071326813 @default.
• W2160848082 cites W2076129724 @default.
• W2160848082 cites W2077890483 @default.
• W2160848082 cites W2078329768 @default.
• W2160848082 cites W2078476027 @default.
• W2160848082 cites W2093126985 @default.
• W2160848082 cites W2101421856 @default.
• W2160848082 cites W2104587229 @default.
• W2160848082 cites W2112571173 @default.
• W2160848082 cites W2118473805 @default.
• W2160848082 cites W2119661887 @default.
• W2160848082 cites W2134326554 @default.
• W2160848082 cites W2147877654 @default.
• W2160848082 cites W2148711092 @default.
• W2160848082 cites W2154544674 @default.
• W2160848082 cites W2167240475 @default.
• W2160848082 cites W2169784712 @default.
• W2160848082 doi "https://doi.org/10.1093/nar/gkn528" @default.
• W2160848082 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2532716" @default.
• W2160848082 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18718929" @default.
• W2160848082 hasPublicationYear "2008" @default.
• W2160848082 type Work @default.
• W2160848082 sameAs 2160848082 @default.
• W2160848082 citedByCount "6" @default.
• W2160848082 countsByYear W21608480822014 @default.
• W2160848082 crossrefType "journal-article" @default.
• W2160848082 hasAuthorship W2160848082A5075917155 @default.
• W2160848082 hasAuthorship W2160848082A5080092101 @default.
• W2160848082 hasBestOaLocation W21608480821 @default.
• W2160848082 hasConcept C100594029 @default.
• W2160848082 hasConcept C121332964 @default.
• W2160848082 hasConcept C12554922 @default.
• W2160848082 hasConcept C128319531 @default.
• W2160848082 hasConcept C178790620 @default.
• W2160848082 hasConcept C181199279 @default.
• W2160848082 hasConcept C185581394 @default.
• W2160848082 hasConcept C185592680 @default.
• W2160848082 hasConcept C195116880 @default.
• W2160848082 hasConcept C2776023528 @default.
• W2160848082 hasConcept C2777028655 @default.
• W2160848082 hasConcept C2778617687 @default.
• W2160848082 hasConcept C33288867 @default.
• W2160848082 hasConcept C54355233 @default.
• W2160848082 hasConcept C552990157 @default.
• W2160848082 hasConcept C55493867 @default.
• W2160848082 hasConcept C62520636 @default.
• W2160848082 hasConcept C74884574 @default.
• W2160848082 hasConcept C86803240 @default.
• W2160848082 hasConcept C91881484 @default.
• W2160848082 hasConcept C91965660 @default.
• W2160848082 hasConceptScore W2160848082C100594029 @default.
• W2160848082 hasConceptScore W2160848082C121332964 @default.
• W2160848082 hasConceptScore W2160848082C12554922 @default.
• W2160848082 hasConceptScore W2160848082C128319531 @default.
• W2160848082 hasConceptScore W2160848082C178790620 @default.
• W2160848082 hasConceptScore W2160848082C181199279 @default.
• W2160848082 hasConceptScore W2160848082C185581394 @default.
• W2160848082 hasConceptScore W2160848082C185592680 @default.
• W2160848082 hasConceptScore W2160848082C195116880 @default.
• W2160848082 hasConceptScore W2160848082C2776023528 @default.
• W2160848082 hasConceptScore W2160848082C2777028655 @default.
• W2160848082 hasConceptScore W2160848082C2778617687 @default.
• W2160848082 hasConceptScore W2160848082C33288867 @default.
• W2160848082 hasConceptScore W2160848082C54355233 @default.
• W2160848082 hasConceptScore W2160848082C552990157 @default.
• W2160848082 hasConceptScore W2160848082C55493867 @default.
• W2160848082 hasConceptScore W2160848082C62520636 @default.
• W2160848082 hasConceptScore W2160848082C74884574 @default.
• W2160848082 hasConceptScore W2160848082C86803240 @default.
• W2160848082 hasConceptScore W2160848082C91881484 @default.
• W2160848082 hasConceptScore W2160848082C91965660 @default.
• W2160848082 hasFunder F4320332161 @default.
• W2160848082 hasIssue "16" @default.
• W2160848082 hasLocation W21608480821 @default.
• W2160848082 hasLocation W21608480822 @default.
• W2160848082 hasLocation W21608480823 @default.

• next