FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160945723> ?p ?o ?g. }

• W2160945723 endingPage "127" @default.
• W2160945723 startingPage "119" @default.
• W2160945723 abstract "In plants, some enzymes of the cytochrome P450 (CYP) superfamily are thought to organize into transient dynamic metabolons to optimize the biosynthesis of bioactive natural products. Metabolon formation may facilitate efficient turnover of labile and toxic intermediates and prevent undesired metabolic cross talk. Two CYPs, CYP79A1 and CYP71E1 involved in the synthesis of dhurrin, were used to assess the possibility to use amphipols (APols) to trap these membrane-bound enzymes in a soluble form in a detergent-free environment. APol surfactants are short polymers composed of a hydrophilic backbone randomly grafted with hydrophobic side chains. An optimal ratio of 1:2 w/w of protein to APol (A8-35) was required for trapping the single transmembrane helices of CYP79A1, CYP71E1, and the electron partner cytochrome P450 oxidoreductase (POR). CYP79A1 and POR retained their individual activity upon A8-35 trapping, whereas a direct interaction between CYP79A1 and POR was hampered, probably due to electrostatic repulsion caused by the negatively charged APol molecules. Upon substitution of POR with NADPH-ferredoxin oxidoreductase and ferredoxin as an electron donor system, the CYPs were shown to be catalytically active. The use of APol surfactants in functional and structural studies of membrane proteins is discussed." @default.
• W2160945723 created "2016-06-24" @default.
• W2160945723 creator A5022237765 @default.
• W2160945723 creator A5030456314 @default.
• W2160945723 creator A5052347289 @default.
• W2160945723 date "2013-01-01" @default.
• W2160945723 modified "2023-10-17" @default.
• W2160945723 title "Amphipol trapping of a functional CYP system" @default.
• W2160945723 cites W1515404266 @default.
• W2160945723 cites W1535871014 @default.
• W2160945723 cites W1554826669 @default.
• W2160945723 cites W1566398256 @default.
• W2160945723 cites W1581549271 @default.
• W2160945723 cites W1588794217 @default.
• W2160945723 cites W1599093857 @default.
• W2160945723 cites W1675570769 @default.
• W2160945723 cites W1895822307 @default.
• W2160945723 cites W1903270425 @default.
• W2160945723 cites W1964469970 @default.
• W2160945723 cites W1971754176 @default.
• W2160945723 cites W1973287828 @default.
• W2160945723 cites W1988348294 @default.
• W2160945723 cites W1988867650 @default.
• W2160945723 cites W1993597465 @default.
• W2160945723 cites W1995855220 @default.
• W2160945723 cites W2001804573 @default.
• W2160945723 cites W2004899462 @default.
• W2160945723 cites W2005574282 @default.
• W2160945723 cites W2007253437 @default.
• W2160945723 cites W2008587056 @default.
• W2160945723 cites W2012580167 @default.
• W2160945723 cites W2018951949 @default.
• W2160945723 cites W2021608380 @default.
• W2160945723 cites W2024609981 @default.
• W2160945723 cites W2026952659 @default.
• W2160945723 cites W2028161167 @default.
• W2160945723 cites W2036324499 @default.
• W2160945723 cites W2037147213 @default.
• W2160945723 cites W2041301497 @default.
• W2160945723 cites W2046499121 @default.
• W2160945723 cites W2049285711 @default.
• W2160945723 cites W2051269343 @default.
• W2160945723 cites W2059936336 @default.
• W2160945723 cites W2060661709 @default.
• W2160945723 cites W2063868052 @default.
• W2160945723 cites W2064931754 @default.
• W2160945723 cites W2065439398 @default.
• W2160945723 cites W2078906576 @default.
• W2160945723 cites W2078925572 @default.
• W2160945723 cites W2082547742 @default.
• W2160945723 cites W2089117176 @default.
• W2160945723 cites W2091068249 @default.
• W2160945723 cites W2092141789 @default.
• W2160945723 cites W2092943003 @default.
• W2160945723 cites W2109196395 @default.
• W2160945723 cites W2114135515 @default.
• W2160945723 cites W2125858923 @default.
• W2160945723 cites W2138696283 @default.
• W2160945723 cites W2171621506 @default.
• W2160945723 cites W2328286101 @default.
• W2160945723 cites W2397472725 @default.
• W2160945723 cites W4248412728 @default.
• W2160945723 doi "https://doi.org/10.1002/bab.1092" @default.
• W2160945723 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23586999" @default.
• W2160945723 hasPublicationYear "2013" @default.
• W2160945723 type Work @default.
• W2160945723 sameAs 2160945723 @default.
• W2160945723 citedByCount "12" @default.
• W2160945723 countsByYear W21609457232014 @default.
• W2160945723 countsByYear W21609457232016 @default.
• W2160945723 countsByYear W21609457232017 @default.
• W2160945723 countsByYear W21609457232018 @default.
• W2160945723 countsByYear W21609457232019 @default.
• W2160945723 countsByYear W21609457232020 @default.
• W2160945723 countsByYear W21609457232021 @default.
• W2160945723 crossrefType "journal-article" @default.
• W2160945723 hasAuthorship W2160945723A5022237765 @default.
• W2160945723 hasAuthorship W2160945723A5030456314 @default.
• W2160945723 hasAuthorship W2160945723A5052347289 @default.
• W2160945723 hasConcept C12554922 @default.
• W2160945723 hasConcept C134621786 @default.
• W2160945723 hasConcept C144647389 @default.
• W2160945723 hasConcept C161624437 @default.
• W2160945723 hasConcept C170493617 @default.
• W2160945723 hasConcept C181199279 @default.
• W2160945723 hasConcept C185592680 @default.
• W2160945723 hasConcept C24530287 @default.
• W2160945723 hasConcept C24840226 @default.
• W2160945723 hasConcept C2779268744 @default.
• W2160945723 hasConcept C2780768313 @default.
• W2160945723 hasConcept C41625074 @default.
• W2160945723 hasConcept C526171541 @default.
• W2160945723 hasConcept C55493867 @default.
• W2160945723 hasConcept C71240020 @default.
• W2160945723 hasConcept C86803240 @default.
• W2160945723 hasConceptScore W2160945723C12554922 @default.
• W2160945723 hasConceptScore W2160945723C134621786 @default.
• W2160945723 hasConceptScore W2160945723C144647389 @default.

• next