FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2160959909> ?p ?o ?g. }

• W2160959909 endingPage "2096" @default.
• W2160959909 startingPage "2089" @default.
• W2160959909 abstract "The outer membrane protein M35 of Moraxella catarrhalis is an antigenically conserved porin. Knocking out M35 significantly increases the MICs of aminopenicillins. The aim of this study was to determine the biological mechanism of this potentially new antimicrobial resistance mechanism of M. catarrhalis and the behaviour of M35 in general stress situations. PCR using m35-specific primers was used to detect the m35 gene in clinical isolates. The m35 mRNA expression of strains 300, O35E and 415 after exposure to amoxicillin and different stress conditions was measured by real-time PCR and normalized in relation to their 16S rRNA expression. The expression of M35 protein was analysed by SDS-PAGE and western blotting. Screening of 52 middle ear isolates resulted in positive PCR products for all tested strains. The analysis of m35 mRNA expression after amoxicillin treatment showed 24%–85% down-regulation compared with the respective amoxicillin-free controls in all three strains tested. Also, analysis of protein concentrations revealed lower M35 expression after growth with amoxicillin. Investigation of M35 during general stress responses showed down-regulation of the porin with growth at 26°C and 42°C, under hyperosmolar stress and under iron restriction. The reduced expression of M35 after aminopenicillin exposure indicates a novel resistance mechanism against aminopenicillins in M. catarrhalis, which may be relevant in vivo. The differences in expression after different stress treatments demonstrate that M35 is involved in general stress responses." @default.
• W2160959909 created "2016-06-24" @default.
• W2160959909 creator A5002287757 @default.
• W2160959909 creator A5032091440 @default.
• W2160959909 creator A5040522972 @default.
• W2160959909 creator A5082417498 @default.
• W2160959909 date "2010-08-27" @default.
• W2160959909 modified "2023-10-18" @default.
• W2160959909 title "Down-regulation of porin M35 in Moraxella catarrhalis by aminopenicillins and environmental factors and its potential contribution to the mechanism of resistance to aminopenicillins" @default.
• W2160959909 cites W149137647 @default.
• W2160959909 cites W1937785817 @default.
• W2160959909 cites W1964980618 @default.
• W2160959909 cites W1965080600 @default.
• W2160959909 cites W1972074942 @default.
• W2160959909 cites W1981267913 @default.
• W2160959909 cites W1989452094 @default.
• W2160959909 cites W1993088565 @default.
• W2160959909 cites W1996764065 @default.
• W2160959909 cites W1998455639 @default.
• W2160959909 cites W1999646223 @default.
• W2160959909 cites W1999690404 @default.
• W2160959909 cites W2009620784 @default.
• W2160959909 cites W2011026301 @default.
• W2160959909 cites W2020181900 @default.
• W2160959909 cites W2020617498 @default.
• W2160959909 cites W2024287671 @default.
• W2160959909 cites W2030309481 @default.
• W2160959909 cites W2035079765 @default.
• W2160959909 cites W2040323664 @default.
• W2160959909 cites W2043312832 @default.
• W2160959909 cites W2046217126 @default.
• W2160959909 cites W2051038130 @default.
• W2160959909 cites W2059608310 @default.
• W2160959909 cites W2059966590 @default.
• W2160959909 cites W2068420419 @default.
• W2160959909 cites W2093807898 @default.
• W2160959909 cites W2096559541 @default.
• W2160959909 cites W2097457871 @default.
• W2160959909 cites W2098621843 @default.
• W2160959909 cites W2102516009 @default.
• W2160959909 cites W2104065934 @default.
• W2160959909 cites W2104086208 @default.
• W2160959909 cites W2104250070 @default.
• W2160959909 cites W2107068916 @default.
• W2160959909 cites W2107329388 @default.
• W2160959909 cites W2107828423 @default.
• W2160959909 cites W2107945331 @default.
• W2160959909 cites W2111979917 @default.
• W2160959909 cites W2116195438 @default.
• W2160959909 cites W2117470119 @default.
• W2160959909 cites W2117567786 @default.
• W2160959909 cites W2119587675 @default.
• W2160959909 cites W2119615529 @default.
• W2160959909 cites W2120061656 @default.
• W2160959909 cites W2120318687 @default.
• W2160959909 cites W2122453558 @default.
• W2160959909 cites W2126161994 @default.
• W2160959909 cites W2126886411 @default.
• W2160959909 cites W2129558427 @default.
• W2160959909 cites W2132417094 @default.
• W2160959909 cites W2133503297 @default.
• W2160959909 cites W2134943351 @default.
• W2160959909 cites W2144670882 @default.
• W2160959909 cites W2150670612 @default.
• W2160959909 cites W2154981049 @default.
• W2160959909 cites W2155097212 @default.
• W2160959909 cites W2158719591 @default.
• W2160959909 cites W2159861657 @default.
• W2160959909 cites W2160001322 @default.
• W2160959909 cites W2162244438 @default.
• W2160959909 cites W2162322404 @default.
• W2160959909 cites W2163017291 @default.
• W2160959909 cites W2164243574 @default.
• W2160959909 cites W2164785244 @default.
• W2160959909 cites W2166221061 @default.
• W2160959909 cites W2167982330 @default.
• W2160959909 cites W2330072357 @default.
• W2160959909 cites W4297572977 @default.
• W2160959909 doi "https://doi.org/10.1093/jac/dkq312" @default.
• W2160959909 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2941675" @default.
• W2160959909 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20801781" @default.
• W2160959909 hasPublicationYear "2010" @default.
• W2160959909 type Work @default.
• W2160959909 sameAs 2160959909 @default.
• W2160959909 citedByCount "19" @default.
• W2160959909 countsByYear W21609599092012 @default.
• W2160959909 countsByYear W21609599092013 @default.
• W2160959909 countsByYear W21609599092014 @default.
• W2160959909 countsByYear W21609599092015 @default.
• W2160959909 countsByYear W21609599092017 @default.
• W2160959909 countsByYear W21609599092021 @default.
• W2160959909 countsByYear W21609599092022 @default.
• W2160959909 countsByYear W21609599092023 @default.
• W2160959909 crossrefType "journal-article" @default.
• W2160959909 hasAuthorship W2160959909A5002287757 @default.
• W2160959909 hasAuthorship W2160959909A5032091440 @default.
• W2160959909 hasAuthorship W2160959909A5040522972 @default.
• W2160959909 hasAuthorship W2160959909A5082417498 @default.

• next