FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2161036637> ?p ?o ?g. }

• W2161036637 abstract "Abstract Background This study aimed to establish a suitable in vitro system for investigating effects of respiratory pathogens and toxins on lung tissue bioenergetics (cellular respiration and ATP content) and caspase activity. Wistar rats and C57Bl/6 mice were anesthetized by sevoflurane inhalation. Lung fragments were then collected and incubated at 37°C in a continuously gassed (with 95% O 2 :5% CO 2 ) Minimal Essential Medium (MEM) or Krebs-Henseleit buffer. Phosphorescence O 2 analyzer that measured dissolved O 2 concentration as a function of time was used to monitor the rate of cellular mitochondrial O 2 consumption. Cellular ATP content was measured using the luciferin/luciferase system. The caspase-3 substrate N -acetyl-asp-glu-val-asp-7-amino-4-methylcoumarin (Ac-DEVD-AMC) was used to monitor intracellular caspase activity; cleaved AMC moieties (reflecting caspase activity) were separated on HPLC and detected by fluorescence. Lung histology and immunostaining with anti-cleaved caspase-3 antibody were also performed. Results For Wistar rats, the values of k c and ATP for 0 < t ≤ 7 h (mean ± SD) were 0.15 ± 0.02 μM O 2 min -1 mg -1 (n = 18, coefficient of variation, Cv = 13%) and 131 ± 69 pmol mg -1 (n = 16, Cv = 53%), respectively. The AMC peak areas remained relatively small despite a ~5-fold rise over 6 h. Good tissue preservation was evident despite time-dependent increases in apoptotic cells. Lung tissue bioenergetics, caspase activity and structure were deleterious in unoxygenated or intermittently oxygenated solutions. Incubating lung tissue in O 2 depleted MEM for 30 min or anesthesia by urethane had no effect on lung bioenergetics, but produced higher caspase activity. Conclusions Lung tissue bioenergetics and structure could be maintained in vitro in oxygenated buffer for several hours and, thus, used as biomarkers for investigating respiratory pathogens or toxins." @default.
• W2161036637 created "2016-06-24" @default.
• W2161036637 creator A5003071786 @default.
• W2161036637 creator A5016680398 @default.
• W2161036637 creator A5021945180 @default.
• W2161036637 creator A5022433901 @default.
• W2161036637 creator A5028539861 @default.
• W2161036637 creator A5037640842 @default.
• W2161036637 creator A5037980313 @default.
• W2161036637 creator A5062448221 @default.
• W2161036637 creator A5072145550 @default.
• W2161036637 creator A5084131807 @default.
• W2161036637 date "2013-01-12" @default.
• W2161036637 modified "2023-10-09" @default.
• W2161036637 title "Lung tissue bioenergetics and caspase activity in rodents" @default.
• W2161036637 cites W1988530973 @default.
• W2161036637 cites W1990098732 @default.
• W2161036637 cites W1992145913 @default.
• W2161036637 cites W1998101020 @default.
• W2161036637 cites W2001582473 @default.
• W2161036637 cites W2035875131 @default.
• W2161036637 cites W2042012186 @default.
• W2161036637 cites W2061490621 @default.
• W2161036637 cites W2076224822 @default.
• W2161036637 cites W2089817405 @default.
• W2161036637 cites W2105665764 @default.
• W2161036637 cites W2115231900 @default.
• W2161036637 cites W2128274032 @default.
• W2161036637 cites W2162435972 @default.
• W2161036637 cites W2166142530 @default.
• W2161036637 cites W2313353429 @default.
• W2161036637 cites W2050939021 @default.
• W2161036637 doi "https://doi.org/10.1186/1756-0500-6-12" @default.
• W2161036637 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3557141" @default.
• W2161036637 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23311890" @default.
• W2161036637 hasPublicationYear "2013" @default.
• W2161036637 type Work @default.
• W2161036637 sameAs 2161036637 @default.
• W2161036637 citedByCount "3" @default.
• W2161036637 countsByYear W21610366372013 @default.
• W2161036637 countsByYear W21610366372014 @default.
• W2161036637 countsByYear W21610366372015 @default.
• W2161036637 crossrefType "journal-article" @default.
• W2161036637 hasAuthorship W2161036637A5003071786 @default.
• W2161036637 hasAuthorship W2161036637A5016680398 @default.
• W2161036637 hasAuthorship W2161036637A5021945180 @default.
• W2161036637 hasAuthorship W2161036637A5022433901 @default.
• W2161036637 hasAuthorship W2161036637A5028539861 @default.
• W2161036637 hasAuthorship W2161036637A5037640842 @default.
• W2161036637 hasAuthorship W2161036637A5037980313 @default.
• W2161036637 hasAuthorship W2161036637A5062448221 @default.
• W2161036637 hasAuthorship W2161036637A5072145550 @default.
• W2161036637 hasAuthorship W2161036637A5084131807 @default.
• W2161036637 hasBestOaLocation W21610366371 @default.
• W2161036637 hasConcept C100206155 @default.
• W2161036637 hasConcept C12519072 @default.
• W2161036637 hasConcept C126322002 @default.
• W2161036637 hasConcept C153911025 @default.
• W2161036637 hasConcept C185592680 @default.
• W2161036637 hasConcept C190283241 @default.
• W2161036637 hasConcept C204232928 @default.
• W2161036637 hasConcept C2775998712 @default.
• W2161036637 hasConcept C2777714996 @default.
• W2161036637 hasConcept C28859421 @default.
• W2161036637 hasConcept C31573885 @default.
• W2161036637 hasConcept C4224716 @default.
• W2161036637 hasConcept C55493867 @default.
• W2161036637 hasConcept C71924100 @default.
• W2161036637 hasConcept C86803240 @default.
• W2161036637 hasConcept C98424977 @default.
• W2161036637 hasConceptScore W2161036637C100206155 @default.
• W2161036637 hasConceptScore W2161036637C12519072 @default.
• W2161036637 hasConceptScore W2161036637C126322002 @default.
• W2161036637 hasConceptScore W2161036637C153911025 @default.
• W2161036637 hasConceptScore W2161036637C185592680 @default.
• W2161036637 hasConceptScore W2161036637C190283241 @default.
• W2161036637 hasConceptScore W2161036637C204232928 @default.
• W2161036637 hasConceptScore W2161036637C2775998712 @default.
• W2161036637 hasConceptScore W2161036637C2777714996 @default.
• W2161036637 hasConceptScore W2161036637C28859421 @default.
• W2161036637 hasConceptScore W2161036637C31573885 @default.
• W2161036637 hasConceptScore W2161036637C4224716 @default.
• W2161036637 hasConceptScore W2161036637C55493867 @default.
• W2161036637 hasConceptScore W2161036637C71924100 @default.
• W2161036637 hasConceptScore W2161036637C86803240 @default.
• W2161036637 hasConceptScore W2161036637C98424977 @default.
• W2161036637 hasIssue "1" @default.
• W2161036637 hasLocation W21610366371 @default.
• W2161036637 hasLocation W21610366372 @default.
• W2161036637 hasLocation W21610366373 @default.
• W2161036637 hasLocation W21610366374 @default.
• W2161036637 hasOpenAccess W2161036637 @default.
• W2161036637 hasPrimaryLocation W21610366371 @default.
• W2161036637 hasRelatedWork W1977139249 @default.
• W2161036637 hasRelatedWork W2000225649 @default.
• W2161036637 hasRelatedWork W2024533066 @default.
• W2161036637 hasRelatedWork W2355416043 @default.
• W2161036637 hasRelatedWork W2359087903 @default.
• W2161036637 hasRelatedWork W2419558278 @default.
• W2161036637 hasRelatedWork W2487472729 @default.

• next